期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 42, 期 4, 页码 1349-1359出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2013.1828
关键词
JB6 cells; rat lung macrophages; cytochrome c; apoptosis-inducing factor; mitochondria
类别
资金
- National Nature Science Foundation of China [81273111]
- Foundations of Innovative Research Team of Educational Commission of Zhejiang Province [T200907]
- Nature Science Foundation of Ningbo city [2012A610185]
- Ningbo Scientific Project [SZX11073]
- Scientific Innovation Team Project of Ningbo [2011B82014]
- Innovative Research Team of Ningbo [2009B21002]
- K.C. Wong Magna Fund in Ningbo University
In this study, apoptosis and related signaling induced by WC-Co nanoparticles were investigated in JB6 cells and rat lung macrophages. Electron spin resonance (ESR) and fluorescent staining indicated that both WC-Co nanoparticles and fine particles stimulated reactive oxygen species (ROS) generation. Catalase exhibited an inhibitory effect on WC-Co nanoparticle-induced ROS as well as mitochondrial membrane permeability damage. Further study indicated that WC-Co nanoparticles elicited higher cytotoxicity and apoptotic induction than fine particles. Western blot analysis showed activation of proapoptotic factors including Fas, Fas-associated protein with death domain (FADD), caspase 3, 8 and 9, BID and BAX. In addition, both cytochrome c and apoptosis-inducing factor (AIF) were upregulated and released from mitochondria to the cytoplasm. Our findings demonstrate that, on a mass basis, WC-Co nanoparticles exhibit higher cytotoxicity and apoptotic induction than fine particles. Apoptosis induced by WC-Co nanoparticles and fine particles involves both extrinsic and intrinsic apoptosis pathways.
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