期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 39, 期 3, 页码 665-672出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2011.1084
关键词
PRAME; microRNA; miR-211; malignant melanoma; cancer-testis antigen
类别
资金
- MIAST (Medical Innovation by Advanced Science and Technology) project
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- [30509013]
- [10326647]
- [16791346]
- Grants-in-Aid for Scientific Research [21791090, 22390071, 23890198] Funding Source: KAKEN
MicroRNAs (miRNAs) are small non-coding RNAs whose aberrations are involved in the initiation and progression of human cancers. To seek unique miRNAs contributing to melanoma tumorigenesis, we investigated the global miRNA expression profile of 7 melanoma cell lines and 3 primary cultures of neonatal human epidermal melanocytes (NHEMs) using the stem-loop real-time PCR method. We found 7 miRNAs that were commonly downregulated and 18 that were upregulated in all of the melanoma cell lines in comparison with the 3 primary cultures of NHEMs. We focused on one commonly downregulated miRNA (miR-211), and analyzed its relationship to the expression of preferentially expressed antigen of melanoma (PRAME) protein, which is a potential target of miR-211. We found that all melanoma cell lines exhibited marked downregulation of miR-211 and upregulation of PRAME mRNA/protein expression in comparison with NHEMs (P < 0.05). A significant inverse correlation between miR-211 and PRAME protein expression was found in melanoma cell lines and primary cultures of NHEMs (correlation coefficient of -0.733, P < 0.05). We demonstrated that overexpression of miR-211 induced a reduction of PRAME protein levels, and confirmed the target specificity between miR-211 and PRAME by luciferase reporter assay. These results suggest that downregulation of miR-211 may be partly involved in aberrant expression of the PRAME protein in melanoma cells.
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