期刊
INTERNATIONAL JOURNAL OF OBESITY
卷 37, 期 11, 页码 1490-1498出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ijo.2013.9
关键词
Adiponectin; mRNA stability; transcription; TNF-alpha; NF-kappa B; PPAR gamma
资金
- NIH [P30DK072476, P20 GM103528]
- National Institute of Health research projects [DK085495, DK068036]
OBJECTIVE: Obesity is associated with an increase in various pro-inflammatory and anti-inflammatory cytokines, but the interplay of these cytokines is incompletely understood. We conducted experiments to test a broader hypothesis that a dynamic interplay of pro-inflammatory and anti-inflammatory cytokines controls lipid storage in adipocytes. DESIGN: Three experiments were designed to test the overall hypothesis that proinflammatory cytokine (for example, tumor necrosis factor-alpha (TNF-alpha) inhibits anti-inflammatory cytokine (for example, adiponectin) activity in an attempt to limit excess lipid accumulation in adipocytes. RESULTS: Experiment one showed that in pro-inflammatory animal models (ap2-P65, ob/ob and high-fat diet-induced obese mice), the increase in TNF-alpha expression was associated with a decrease in adiponectin expression. Experiment two showed that in 3T3-L1 adipocytes, TNF-alpha significantly reduced lipid accumulation and glucose uptake induced by adiponectin, and increased lipolysis. Experiment three showed that in 3T3-L1 adipocytes, TNF-alpha reduced mRNA and protein expression of adiponectin. Adiponectin gene transcription and mRNA stability were both reduced by TNF-alpha. The expression of peroxisome proliferator-activated receptor gamma, an activator of adiponectin gene promoter, was reduced by TNF-alpha. The inhibitory activity of TNF-a was blocked by the chemical inhibitors of NF-kappa B and super suppressor I kappa B alpha. CONCLUSIONS: TNF-alpha opposes the action of adiponectin in the regulation of lipid metabolism, and inhibits adiponectin expression at transcriptional and post-transcriptional levels. The results suggest that pro-inflammatory cytokine inhibit anti-inflammatory cytokine in adipocytes to reduce lipid storage. This suggests a potential role of anti-inflammatory cytokines in the control of adipose tissue expansion.
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