Article
Agriculture, Dairy & Animal Science
Dingshuang Chen, Yaqiu Lin, Nan Zhao, Yong Wang, Yanyan Li
Summary: In this study, it was discovered that Hoxa5 promotes adipogenesis and inhibits proliferation in goat subcutaneous preadipocyte cells. This provides a theoretical basis for understanding the regulation of goat adipocyte differentiation and proliferation mediated by Hoxa5.
Article
Agriculture, Dairy & Animal Science
Weiguo Cui, Qian Zhang, Hanqiong Wang, Xiaohan Zhang, Ming Tian, Di Liu, Xiuqin Yang
Summary: This study reveals the role of HOXC8 in fat accumulation by showing its association with fat contents in muscles and its promotion of preadipocyte proliferation and differentiation. Mechanisms underlying HOXC8's regulation of preadipocyte proliferation and differentiation are identified, highlighting the IL-17 signaling pathway as an important mediator of fat accumulation regulated by HOXC8.
Review
Medical Laboratory Technology
Huiqing Liu, Xingqi Meng, Jinyuan Wang, Yi Wei, Jinru Tang, Xiaocan Lei, Weiguo He, Suyun Li
Summary: PCOS is an endocrinemetabolic disease affecting reproductive aged women, potentially leading to various complications. Recent studies have identified a protein called SAA that may be associated with PCOS, serving as a potential inflammatory biomarker.
CLINICA CHIMICA ACTA
(2021)
Article
Medicine, Research & Experimental
Bahareldin Ali Abdalla, Xiaolan Chen, Kan Li, Jie Chen, Zhenhua Yi, Xiquan Zhang, Zhenhui Li, Qinghua Nie
Summary: The study demonstrated that FoxO6 controls preadipocyte proliferation, apoptosis, and early adipogenesis in chicken. It regulates cell-cycle related markers and targets CCNG2 during preadipocyte proliferation. FoxO6 also induces preadipocyte apoptosis and suppresses key regulators of early adipogenesis. These findings provide new insights for further research on obesity-related mechanisms.
Article
Biochemistry & Molecular Biology
Dipanjan Banerjee, Debarun Patra, Archana Sinha, Soumyajit Roy, Rajat Pant, Raktim Sarmah, Rajdeep Dutta, Sarada Kanta Bhagabati, Kulbhushan Tikoo, Durba Pal, Suman Dasgupta
Summary: Research suggests that the interaction between macrophages and adipocytes in obese individuals can lead to insulin resistance. This study found that a lipid-induced compound called CyPA significantly impairs adipocyte functions and insulin sensitivity. Inhibition of CyPA reduces adipose tissue inflammation and restores adipocyte function, improving insulin sensitivity. Mechanistically, the interaction between CyPA and its receptor CD147 activates a signaling pathway that promotes adipocyte inflammation and inhibits adipocyte differentiation. Inhibiting CyPA or CD147 can restore adipose tissue function and insulin sensitivity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Environmental Sciences
Yong Pu, Elvis Ticiani, Anita A. Waye, Kunzhe Dong, Huanmin Zhang, Almudena Veiga-Lopez
Summary: Gestational exposure to BPA can accelerate adipogenic differentiation in female ovine fetal preadipocytes and alter pathways associated with extracellular matrix components.
Article
Cell Biology
Min Chen, Soochi Kim, Liang Li, Sourav Chattopadhyay, Thomas A. Rando, Brian J. Feldman
Summary: Through single-cell RNA sequencing, a population of CD31- CD45- cells with a specific expression profile is discovered in the stromal vascular fraction of adipose tissue. This population is placed on an epistatic pathway upstream of preadipocytes and functional properties with broad implications, including physiological mechanisms regulating adipogenesis, are revealed.
Article
Biochemistry & Molecular Biology
L. M. Kotze-Horstmann, D. T. Bedada, R. Johnson, L. Mabasa, H. Sadie-Van Gijsen
Summary: The study evaluated the effects of a green Rooibos extract on improving metabolic dysregulation caused by obesity. The extract had an impact on the associations at the systemic, adipose tissue, and cellular levels.
Article
Toxicology
Elvis Ticiani, Yong Pu, Madison White, Victoria Adomshick, Almudena Veiga-Lopez
Summary: Butyltins and phenyltins, widely used organometallic chemicals, have been found to stimulate adipogenic differentiation. Among them, tributyltin (TBT) is the most potent inducer, followed by triphenyltin (TPT) and dibutyltin (DBT). However, TPT and DBT can weaken the adipogenic effect of TBT. These findings indicate the antagonistic effects among organotins and the involvement of PPAR gamma signaling in their adipogenic outcomes.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Oncology
Manisha du Plessis, Tanja Andrea Davis, Daniel Wilhelm Olivier, Willem Johan Simon de Villiers, Anna-Mart Engelbrecht
Summary: This study aimed to investigate the role of SAA in the regulation of signaling pathways and autophagy in breast cancer. The results showed that SAA overexpression activated the ERK signaling pathway and downregulated the expression of the PI3K pathway protein. SAA knockout inhibited Akt activity. Furthermore, SAA overexpression downregulated autophagy and increased the expression of SQSTM1/p62 in MCF7 cells, while SAA knockout induced autophagy. SAA overexpression increased cell viability and the expression of proliferation marker. SAA knockout altered the inflammatory profile and promoted resistance to apoptosis and necrosis through the regulation of autophagy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Zhongchang Hu, Jian Wu, Lihong Qin, Haiguo Jin, Yang Lv, Ran Zhang, Cheng Xiao, Yang Cao, Yumin Zhao
Summary: ALDH1A1 plays a significant regulatory role in the differentiation of preadipocyte cells in Yan Yellow Cattle. Modulation of ALDH1A1 gene expression can affect acetaldehyde oxidation and adipocyte development, with changes in lipid accumulation and triglyceride production observed in mature adipocytes. The protein expression levels of ALDH1A1 and adipogenic factors corresponded to the mRNA levels, indicating an important role of ALDH1A1 in fat cell growth and development.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Briana K. Clifford, Nadia M. L. Amorim, Nadeem O. Kaakoush, Lykke Boysen, Nicodemus Tedla, David Goldstein, Edna C. Hardeman, David Simar
Summary: This study aimed to investigate the effects of irradiation and diet on the gut microbiota and metabolic or immune functions. The results showed a compounding negative impact of irradiation and a high-fat diet on the metabolic and immune profiles of adipose tissue, along with altered microbiota. These findings are important for the diagnosis and prevention of metabolic complications in cancer survivors exposed to radiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Benjarat Janson, Janjira Prasomthong, Wachirawadee Malakul, Tantip Boonsong, Sakara Tunsophon
Summary: This study demonstrated that roselle extract can reduce body weight, food intake, lipid profiles, inflammatory cytokines, lipid peroxidation, serum leptin, insulin, and duodenal glucose absorption, while significantly increasing glucose uptake of adipose tissue and muscle compared to a high-fat diet group. Roselle can prevent lipid accumulation by suppressing differentiation of 3T3-L1 adipocyte through downregulating adipogenic gene expression, providing an alternative approach for preventing obesity-related insulin resistance.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Zhongchang Hu, Jian Wu, Lihong Qin, Haiguo Jin, Yang Cao, Yumin Zhao
Summary: The study revealed that the expression levels of the IGFBP7 gene are specifically regulated during the adipocyte differentiation in Yan Yellow Cattle. Overexpression and RNA interference of the IGFBP7 gene had significant effects on lipid accumulation and triglyceride production in mature adipocytes.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Camila O. de Souza, Vivian A. Paschoal, Xuenan Sun, Lavanya Vishvanath, Qianbin Zhang, Mengle Shao, Toshiharu Onodera, Shiuhwei Chen, Nolwenn Joffin, Lorena M. A. Bueno, Rana K. Gupta, Da Young Oh
Summary: This study reveals that the lack of GPR92 in islet macrophages can lead to beta cell dysfunction, disrupting glucose homeostasis. Additionally, stimulation of GPR92 inhibits islet inflammation and increases insulin secretion.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biotechnology & Applied Microbiology
Najla Adel Saleh, Michele Patricia Rode, Jelver Alexander Sierra, Adny Henrique Silva, Juliano Andreoli Miyake, Fabiola Branco Filippin-Monteiro, Tania Beatriz Creczynski-Pasa
Summary: The study used a three-dimensional co-culture model to mimic and study the tumor microenvironment of malignant melanoma. It was found that M2 macrophages induced an anti-inflammatory microenvironment in heterospheroids, fibroblast cells supported the formation of the extracellular matrix, and a higher percentage of melanoma CD271 was observed in this model. Additionally, melanoma spheroids responded differently to dacarbazine compared to 2D melanoma culture.
Article
Public, Environmental & Occupational Health
Okunola A. Alabi, Adny H. Silva, Michele P. Rode, Carine dal Pizzol, Angela Machado de Campos, Fabiola B. Filippin-Monteiro, Adekunle A. Bakare, Tania B. Creczynski-Pasa
Summary: Increased production and use of different types of nanoparticles have led to increased environmental release and potential detrimental effects. Co-exposure to solid lipid nanoparticles (SLNs) and superparamagnetic iron oxide nanoparticles (SPIONs) showed synergistic, antagonistic, and additive effects on cytotoxicity, with apoptosis triggered by ROS and disturbances in Delta psi M as probable mechanisms. Possible cytotoxic interaction between the two types of nanoparticles was observed.
TOXICOLOGY AND INDUSTRIAL HEALTH
(2021)
Article
Cell Biology
Bas Brouwers, Edson Mendes de Oliveira, Maria Marti-Solano, Fabiola B. F. Monteiro, Suli-Anne Laurin, Julia M. Keogh, Elana Henning, Rebecca Bounds, Carole A. Daly, Shane Houston, Vikram Ayinampudi, Natalia Wasiluk, David Clarke, Bianca Plouffe, Michel Bouvier, M. Madan Babu, I. Sadaf Farooqi, Jacek Mokrosinski
Summary: Research on melanocortin-4 receptor (MC4R) mutations associated with obesity reveals the importance of endocytosis, intracellular trafficking, and homodimerization in regulating MC4R function, supporting the development of novel therapies for weight loss.
Article
Parasitology
Maria Luiza Carneiro Buchele, Fabiola Branco Filippin-Monteiro, Barbara de Lima, Carolina de Jesus Camargo, Jelver Alexander Sierra Restrepo, Liliete Canes Souza, Tania Beatriz Creczynski-Pasa, Karin Silva Caumo
Summary: The study demonstrated that preheating Amphotericin B and combining it with a thermoreversible in situ gelling ophthalmic system showed promising results in the treatment of Acanthamoeba keratitis, with high efficacy and low cytotoxicity.
Article
Pharmacology & Pharmacy
Allisson Jhonatan Gomes Castro, Luisa Helena Cazarolli, Marisa Jadna Silva Frederico, Betina Fernanda Dambros, Francieli Kanumfre de Carvalho, Veronica Aiceles de Medeiros Pinto, Cristiane da Fonte Ramos, Fabiola Branco Filippin Monteiro, Moacir Geraldo Pizzolatti, Fatima Regina Mena Barreto Silva
Summary: THO reduces hyperglycemia by increasing serum insulin and hepatic glycogen content. The mechanism of action in adipose tissue for glucose uptake is suggested to be via GLUT4 translocation stimulation mediated by a p38-dependent mechanism.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Tessa M. Cacciottolo, Elana Henning, Julia M. Keogh, Pierre Bel Lassen, Katherine Lawler, Rebecca Bounds, Rachel Ahmed, Aliki Perdikari, Edson Mendes de Oliveira, Miriam Smith, Edmund M. Godfrey, Elspeth Johnson, Leanne Hodson, Karine Clement, Agatha A. van der Klaauw, I. Sadaf Farooqi
Summary: SRC-1 variant carriers exhibit hyperphagia in childhood, severe obesity, and clinical features of partial hormone resistance. The presence of adipose tissue fibrosis and hepatic fibrosis in young patients suggests that close monitoring for the early development of obesity-associated metabolic complications is warranted.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Medicine, General & Internal
Edson Mendes de Oliveira, Julia M. Keogh, Fleur Talbot, Elana Henning, Rachel Ahmed, Aliki Perdikari, Rebecca Bounds, Natalia Wasiluk, Vikram Ayinampudi, Ines Barroso, Jacek Mokrosinski, Deepthi Jyothish, Sharon Lim, Sanjay Gupta, Melanie Kershaw, Cristina Matei, Praveen Partha, Tabitha Randell, Antoinette McAulay, Louise C. Wilson, Tim Cheetham, Elizabeth C. Crowne, Peter Clayton, I. Sadaf Farooqi
Summary: Variants in the GNAS gene have been linked to childhood obesity, showing the importance of genetic factors in this condition. Mutations in GNAS affect GPCR signaling pathways, leading to a wide range of clinical manifestations. Early diagnosis through genetic screening and targeted treatment with melanocortin agonists may improve outcomes for children with severe obesity.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Aliki Perdikari, Tessa Cacciottolo, Elana Henning, Edson Mendes de Oliveira, Julia M. Keogh, I. Sadaf Farooqi
Summary: Obesity is a major health issue, but the structure and function of human adipose tissue have been difficult to understand due to the limitations in visualizing cellular components. In this study, we developed a protocol using staining and clearing techniques to visualize human subcutaneous white adipose tissue and successfully visualized sympathetic nerve terminals.
Article
Pharmacology & Pharmacy
Edson Mendes de Oliveira, Jacqueline C. Silva, Thais P. Ascar, Silvana Sandri, Alexandre F. Marchi, Silene Migliorini, Helder T. Nakaya, Ricardo A. Fock, Ana Campa
Summary: This study investigated the effects of intense and recurring endotoxemia on weight gain and associated metabolic complications. The results showed that acute endotoxemia led to increased weight gain, larger fat depots, higher levels of leptin and insulin, and impaired insulin sensitivity when mice were on a high-fat diet. Furthermore, endotoxemia caused a long-lasting increase in the expression of inflammatory markers in adipose tissue, which could connect inflammation to increased susceptibility to weight gain and impaired glucose homeostasis.
Article
Environmental Sciences
Carolina Dumke de Siqueira, Alabi Okunola Adenrele, Ana Carolina Rabello de Moraes, Fabiola Branco Filippin-Monteiro
Summary: This study aimed to determine the concentration of bisphenol A (BPA) in the colostrum of post-partum women in South Brazil. The results showed a high concentration of BPA in all breast milk samples, which might be due to the consumption of foods packaged in plastic packaging. This has significant public health implications, as the high concentration of BPA could lead to serious health problems in both the lactating mothers and the babies fed with BPA-contaminated breast milk.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Layzon A. Lemos da Silva, Louis P. Sandjo, Laura S. Assuncao, Anne N. Prigol, Carolina D. de Siqueira, Tania B. Creczynski-Pasa, Marcus T. Scotti, Luciana Scotti, Fabiola B. Filippin-Monteiro, Maique W. Biavatti
Summary: This study investigates the sensitivity of semisynthetic sesquiterpene lactone (SL) derivatives by preparing them under different pH conditions and evaluating their anti-inflammatory and antiproliferative activities. Results indicate that some of these derivatives exhibit significant cytotoxic activity and selectivity against human melanoma cells. They also demonstrate the ability to reduce the release of interleukin-6 (IL-6) and nitrite (NOx) in pre-treated macrophages. Overall, the study suggests that acidic and alkaline extraction processes can promote the formation of SLs.
Article
Biochemistry & Molecular Biology
Yang He, Bas Brouwers, Hesong Liu, Hailan Liu, Katherine Lawler, Edson Mendes de Oliveira, Dong-Kee Lee, Yongjie Yang, Aaron R. Cox, Julia M. Keogh, Elana Henning, Rebecca Bounds, Aliki Perdikari, Vikram Ayinampudi, Chunmei Wang, Meng Yu, Longlong Tu, Nan Zhang, Na Yin, Junying Han, Nikolas A. Scarcelli, Zili Yan, Kristine M. Conde, Camille Potts, Jonathan C. Bean, Mengjie Wang, Sean M. Hartig, Lan Liao, Jianming Xu, Ines Barroso, Jacek Mokrosinski, Yong Xu, I. Sadaf Farooqi
Summary: Serotonin 2C receptor (5-HT2CR) plays a role in the regulation of human appetite, weight and behavior. Loss-of-function variants in the HTR2C gene are associated with obesity and maladaptive behavior. Activation of appetite-suppressing neurons is impaired in mice carrying HTR2C variants.
Article
Genetics & Heredity
Leticia de Oliveira Souza Bratti, Bruno Fonseca Nunes, Daphany Marah Gorges, Fabiola Branco Filippin-Monteiro
Summary: In recent years, there has been significant research on understanding the pathophysiology of obesity, particularly in relation to satiety signals in the hypothalamus and hormonal signaling in orexigenic and anorexigenic neurons. Mutations in genes encoding proteins, especially peptide receptors, lead to different phenotypes. Identifying allelic variants and central genes related to obesity can provide insights into the proteins involved in these processes and aid in early laboratory diagnosis.
Article
Pharmacology & Pharmacy
Debora Mocellin, Leticia de Oliveira Souza Bratti, Adny Henrique Silva, Laura Sartori Assuncao, Iara Fabricia Kretzer, Fabiola Branco Filippin-Monteiro
Summary: This study examined the effects of serum from obese patients on gene expression in melanoma cell lines. The results showed that serum from obese patients enhanced the migration capacity and increased NRAS expression levels in melanoma cells. TNF-alpha, an obesity-related cytokine, had only a modest contribution to melanoma cell migration capacity.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)