Article
Multidisciplinary Sciences
Bryan E. Fowler, Lindsey J. Macpherson
Summary: Advances in genetically encoded calcium indicators (GECIs) have revolutionized in vivo functional imaging by monitoring the responses of individual cells to various stimuli in real time. Imaging the taste-evoked responses of geniculate ganglion neurons with GCaMP has provided important information about their tuning profiles and detecting taste miswiring phenotypes. This technique is a powerful tool for monitoring the activity of peripheral gustatory neurons and complements traditional methods like whole-nerve chorda tympani recordings or taste behavior assays.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemical Research Methods
Xinyang Li, Guoxun Zhang, Jiamin Wu, Yuanlong Zhang, Zhifeng Zhao, Xing Lin, Hui Qiao, Hao Xie, Haoqian Wang, Lu Fang, Qionghai Dai
Summary: DeepCAD is a self-supervised deep-learning approach for denoising calcium imaging data that enhances spatiotemporal performance, improves signal-to-noise ratio, and facilitates neuron extraction and spike inference.
Article
Biology
Jormay Lim, Hsiao-Hsin Tai, Wei-Hao Liao, Ya-Cherng Chu, Chen-Ming Hao, Yueh-Chun Huang, Cheng-Han Lee, Shao-Shien Lin, Sherry Hsu, Ya-Chih Chien, Dar-Ming Lai, Wen-Shiang Chen, Chih-Cheng Chen, Jaw-Lin Wang
Summary: Accumulating evidence suggests that transcranial low-intensity ultrasound could be a non-invasive neural modulation tool for treating brain diseases. Low-intensity ultrasound can activate neurons in the mouse brain and induce adult neurogenesis in specific regions. ASIC1a is identified as the molecular determinant involved in the mechano-signaling of low-intensity ultrasound for neural activation in the mouse brain.
Article
Neurosciences
Han -Sol Lee, Jin-Hee Han
Summary: The hippocampus is essential for retrieving contextual memories, and the activation of a specific group of neurons in the dorsal CA1 is necessary for memory retrieval. Individual neurons and synchronous activity patterns in the dCA1 neuronal network play a critical role in representing contextual memory recall.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemical Research Methods
Laila Ananda Blomer, Luiza Filipis, Marco Canepari
Summary: The low-affinity Ca2+ indicator Cal-520FF has been found to offer superior signal-to-noise ratio and optimal characteristics for recording fast Ca2+ signals and measuring the kinetics of Ca2+ currents, making it the optimal commercial indicator for ultrafast Ca2+ imaging applications.
JOURNAL OF FLUORESCENCE
(2021)
Article
Chemistry, Multidisciplinary
Xiao Wei, Haoqi Li, Hao Li, Zewen Zuo, Fengqi Song, Xi Kang, Manzhou Zhu
Summary: Metal nanoclusters can be transformed into new nanoclusters with customized structures and enhanced performance using efficient strategies. However, tracking the nanocluster transformations has been challenging. In this study, a slice visualization approach was used to monitor the transformation of Au1Ag24(SR)(18) to Au1Ag30(SR)(20) nanoclusters. The mechanism of nanocluster structure growth was mapped and intermediate products were observed with atomic precision. This approach not only provides a platform for studying structure-property correlations but also offers a means to obtain clear information on nanocluster structure evolution.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Neurosciences
Tamas Papp, Zsuzsanna Ferenczi, Bernadette Szilagyi, Matyas Petro, Angelika Varga, Eva Kokai, Ervin Berenyi, Gabor Olah, Gabor Halmos, Peter Szucs, Zoltan Meszar
Summary: The regulation of neuronal differentiation and synaptogenesis requires precise coordination of intrinsic and extrinsic factors. While ultrasound is known to acutely alter neuronal migration and activity, its chronic effect on neuronal behavior or morphology is not well understood. This study investigated the neurobiological effects of high-frequency ultrasound on cortical pyramidal cell morphology in a transgenic mouse model, finding that low-intensity ultrasound stimulation led to increased dendritic branching and activation of certain signaling pathways.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Endocrinology & Metabolism
Sandra Postic, Marko Gosak, Wen-Hao Tsai, Johannes Pfabe, Srdjan Sarikas, Andraz Stozer, Dean Korosak, Shi-Bing Yang, Marjan Slak Rupnik
Summary: Extracellular pH has an impact on the activation and collective activity of pancreatic beta cells. Lowered pH can activate a subset of beta cells and enhance their average activity and insulin release, even though it reduces functional connectivity and increases functional segregation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Optics
Meng Li, Liheng Bian, Jun Zhang
Summary: This study reports a multi-slice coded coherent diffraction imaging (CDI) technique that enables high-fidelity reconstruction of thick objects. It overcomes the limitations of traditional coded CDI when dealing with thick objects.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Da Guo, Aysen Gurkan Ozer, Marylka Yoe Uusisaari
Summary: The inferior olive (IO) is considered crucial for motor control as the only source of climbing fibers forming one of the cerebellar input pathways. A new surgical method has been developed to obtain high-level calcium imaging data from the IO network activity in living animals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Neurosciences
Serika Yamada, Yan Wang, Hiromu Monai
Summary: Visualization and tracking of information flow in the brain's broader area can be achieved through fluorescence Ca2+ imaging, which allows for simultaneous visualization of brain cell activities. Using transgenic animals that express Ca2+-sensitive fluorescent proteins, brain activities can be observed at a larger scale for a longer time, providing practical applications for monitoring wide-field information flow and evaluating cortical function in disease models.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Neurosciences
Fabio Miazzi, Kalpana Jain, Sabine Kaltofen, Jan E. Bello, Bill S. Hansson, Dieter Wicher
Summary: This article presents different preparations to study odor information processing in the olfactory system of fruit flies, including in vivo imaging and embedding in silica hydrogel. The results show that the odor response dynamics under different conditions are qualitatively similar.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biology
Zhengchao Xu, Zhao Feng, Mengting Zhao, Qingtao Sun, Lei Deng, Xueyan Jia, Tao Jiang, Pan Luo, Wu Chen, Ayizuohere Tudi, Jing Yuan, Xiangning Li, Hui Gong, Qingming Luo, Anan Li
Summary: The study utilized viral tracing combined with imaging techniques to reveal a comprehensive whole-brain atlas of inputs and outputs of glutamatergic and GABAergic neurons in the dorsal raphe nucleus (DR) and median raphe nucleus (MR). The findings showed divergent projection patterns and differences in critical brain regions among glutamatergic and GABAergic neurons within the same raphe nucleus, shedding light on the anatomical architecture and behavioral functions of these nuclei.
Article
Neurosciences
Ivan Marchionni, Nadia Pilati, Angelo Forli, Michele Sessolo, Angelita Tottene, Daniela Pietrobon
Summary: Migraine is a complex brain disorder characterized by unilateral headache and dysfunction in multisensory information processing. This study investigates the cellular and circuit mechanisms underlying familial hemiplegic migraine (FHM) in mouse models. The findings suggest that enhanced excitatory neurotransmission and unaltered inhibitory neurotransmission may contribute to the pathogenesis of FHM.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Nazanin Doostdar, Joseph Airey, Carola Radulescu, Leire Melgosa-Ecenarro, Nawal Zabouri, Pavlina Pavlidi, Maksym Kopanitsa, Takashi Saito, Takaomi Saido, Samuel J. Barnes
Summary: The adult neocortex has the capacity to reorganize across multiple spatial scales, with plastic reorganization occurring at the level of sensory maps, neuronal assemblies, and synaptic ensembles. Using calcium imaging, the study demonstrates network reorganization in animals undergoing behavioral assessment, measures network dynamics at both stable and transient functional assemblies, and evaluates synaptic ensembles of dendritic spines in vivo. The imaging techniques can be used to measure parallel plasticity processes in healthy brains and preclinical models of disease.
Review
Neurosciences
Ai Nakashima, Naoki Ihara, Yuji Ikegaya, Haruki Takeuchi
Summary: The article summarizes the current understanding of mechanisms underlying topographic map development in the mouse olfactory system, emphasizing the role of neural activity in instructing the map refinement process.
NEUROSCIENCE RESEARCH
(2021)
Review
Neurosciences
Satoshi Hirata, Toru Betsuyaku, Kazuo Fujita, Tamami Nakano, Yuji Ikegaya
Summary: Humans, nonhuman animals, and infants all possess specific mental time travel abilities, indicating the presence of a developmental basis for fully mature mental time travel.
NEUROSCIENCE RESEARCH
(2021)
Article
Neurosciences
Satoshi Iwasaki, Yuji Ikegaya
Summary: This study demonstrates that precise activation of a specific neuron ensemble in the hippocampus is crucial for memory retrieval, while noisy activity of memory-irrelevant neurons can easily disrupt this process.
Review
Neurosciences
Tasuku Araki, Yuji Ikegaya, Ryuta Koyama
Summary: Hippocampal neurogenesis continues throughout life and is tightly controlled by environmental conditions, with microglia and astrocytes playing key roles in modulating the process. Glial cells release factors that support adult neurogenesis under normal conditions, but can also contribute to inflammation and hinder neurogenesis in diseases like Alzheimer's, depression, and epilepsy.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Behavioral Sciences
Dorita H. F. Chang, Nikolaus F. Troje, Yuji Ikegaya, Ichiro Fujita, Hiroshi Ban
Summary: The study aimed to understand the spatiotemporal characteristics of biological motion perception. Univariate response components were observed at around 200ms and 650ms post-stimulus onset, with multivariate patterns specific to form discriminated as early as 100ms. Early responses to biological motion likely originate from occipital cortex, while later responses likely come from extrastriate body areas.
Article
Pharmacology & Pharmacy
Airi Yoshimoto, Kotaro Yamashiro, Takeshi Suzuki, Yuji Ikegaya, Nobuyoshi Matsumoto
Summary: This study investigated the effects of ramelteon on sleep and neural oscillatory activities in rats, finding that ramelteon promoted NREM sleep and enhanced fast gamma power in the primary motor cortex. Compared to benzodiazepine treatment, ramelteon modulated neural oscillations in a unique way that may contribute to offline information processing during sleep.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hideyoshi Igata, Yuji Ikegaya, Takuya Sasaki
Summary: During the learning process, hippocampal cell ensembles exhibit dynamic changes in replay patterns, incorporating salient locations and reward-related contexts. The directionality of replays gradually varies, including the replay of optimized paths that have never been exploited by the animals, indicating a prioritized replay of significant experiences.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Zhiwen Zhou, Kazuki Okamoto, Junya Onodera, Toshimitsu Hiragi, Megumi Andoh, Masahito Ikawa, Kenji F. Tanaka, Yuji Ikegaya, Ryuta Koyama
Summary: The study demonstrates that increasing cAMP levels in astrocytes can induce synaptic plasticity and affect memory formation and retention, mediated through the astrocyte-neuron lactate shuttle. This provides a tool to modulate astrocytic cAMP in vivo and sheds light on the role of astrocytic cAMP in brain function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Kazuki Okamoto, Teppei Ebina, Naoki Fujii, Kuniaki Konishi, Yu Sato, Tetsuhiko Kashima, Risako Nakano, Hiroyuki Hioki, Haruki Takeuchi, Junji Yumoto, Masanori Matsuzaki, Yuji Ikegaya
Summary: The newly introduced Tb3+-doped glass material emits green fluorescence in biological experiments and is compatible with conventional research environments. Using micropipettes made of Tb3+-doped glass allows for various operations on GFP-labeled cells under real-time fluorescence microscopic control.
Article
Biochemistry & Molecular Biology
Yu Shikano, Yuji Ikegaya, Takuya Sasaki
Summary: The study found that hippocampal and striatal neurons are able to gradually change their firing rates and transiently increase firing rates at specific time points, representing successive time points on the order of minutes during the process of animals learning a time-reward relationship. These minute-encoding patterns progressively develop and undergo flexible scaling in parallel with timing behavior.
Article
Neurosciences
Satoshi Iwasaki, Takuya Sasaki, Yuji Ikegaya
Summary: Memorizing the locations of environmental cues is crucial for survival and is dependent on the hippocampus. This study revealed that beta oscillations in the hippocampal CA1 region are involved in the memory encoding of object-location associations, with stronger power correlating with better performance in memory retrieval.
Article
Engineering, Biomedical
Yusuke Watanabe, Mami Okada, Yuji Ikegaya
Summary: The study introduces an automatic method for detecting hippocampal ripples, effectively distinguishing ripples from myoelectric noise and detecting ripples accurately even when animals are moving. The use of deep learning techniques achieved high accuracy and performance evaluation metrics.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Yutaka Hoshi, Koji Shibasaki, Philippe Gailly, Yuji Ikegaya, Ryuta Koyama
Summary: Social stress-induced hyperthermia activates TRPV4 in NSCs, impairing hippocampal neurogenesis. Specific knockout of TRPV4 in NSCs or removal of microglia alleviates the impairment of hippocampal neurogenesis.
Article
Multidisciplinary Sciences
Asako Noguchi, Roman Huszar, Shota Morikawa, Gyorgy Buzsaki, Yuji Ikegaya
Summary: The activation of inhibitory neurons prior to hippocampal sharp-wave ripples regulates the order of spikes, which is crucial for memory storage.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Asako Noguchi, Kotaro Yamashiro, Nobuyoshi Matsumoto, Yuji Ikegaya
Summary: A deep neural network model can predict theta oscillations in the mouse hippocampal CA1 area based on membrane potentials of as few as three pyramidal cells. Theta oscillations are characteristic local field potentials (LFPs) in the hippocampus that occur during spatial navigation, exploratory sniffing, and rapid eye movement sleep. This study found a weak but significant correlation between the temporal evolution of theta oscillations in membrane potentials and LFP theta oscillations, and a deep neural network was able to predict LFP waveforms based on the membrane potentials of three neurons.
COMMUNICATIONS BIOLOGY
(2023)
