Article
Biochemistry & Molecular Biology
Michal Marcinkowski, Tomas Pilzys, Damian Garbicz, Jan Piwowarski, Kaja Przygonska, Maria Winiewska-Szajewska, Karolina Ferenc, Oleksandr Skorobogatov, Jaroslaw Poznanski, Elzbieta Grzesiuk
Summary: FTO is an N-6-methyladenosine demethylase which can remove methyl groups from nucleic acids, forming complexes with other proteins. Research indicates an interaction between FTO and calmodulin (CaM), influencing calcium-binding loops in CaM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anastasiia Bohush, Wieslawa Lesniak, Serge Weis, Anna Filipek
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the formation of Lewy bodies and loss of dopaminergic neurons. Changes in intracellular Ca2+ levels can have serious consequences on cells, with calmodulin playing a crucial role in regulating neuronal plasticity. Therefore, alterations in the activity of calmodulin and its binding proteins may be linked to the development and progression of PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Ji Cao, Jingjing Zhang, Jianan Qian, Xue Wang, Wei Zhang, Xiangfan Chen
Summary: This study investigated the effect of RIPK3-regulated CaMKII on necroptosis in heart failure and its mechanism. The results showed that depletion of RIPK3 alleviated cardiac dysfunction, CaMKII activation, and necroptosis. These findings provide valuable insights into the pathogenesis and treatment strategy of heart failure.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Carolyn Nicole Brown, Nicole L. Rumian, Jonathan E. Tullis, Steven J. Coultrap, K. Ulrich Bayer
Summary: A beta shares similarity with the CaMKII regulatory domain, but does not directly affect CaMKII function. The impairment of CaMKII induced by A beta at synapses is mediated by indirect signaling events.
Article
Multidisciplinary Sciences
Premchand Rajeev, Nivedita Singh, Adel Kechkar, Cory Butler, Narendrakumar Ramanan, Jean-Baptiste Sibarita, Mini Jose, Deepak Nair
Summary: Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. The molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Additionally, the nanoscale molecular signatures of these signaling islands and phase transitions are altered in response to changes in cytosolic Ca2+, showing differential sensitivities to Ca2+ bound Calmodulin. SAP97/hDLG forms nanosized molecular domains in both heterologous cells and hippocampal pyramidal neurons, with molecular signatures and exchange kinetics being modulated by the availability of alternatively spliced isoforms.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Tuan A. Nguyen, Henry L. Puhl, Kirk Hines, Daniel J. Liput, Steven S. Vogel
Summary: The regulation and modulation of synaptic functions rely on a coordinated choreography of protein conformational changes and interactions, which are influenced by intracellular calcium dynamics. By using time-lapse Forster resonance energy transfer, we can study the dynamics of both conformational changes and protein-protein interactions simultaneously under physiological conditions.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Tzu-Kang Lin, Chi-Feng Hung, Jing-Ru Weng, Ting-Yang Hsieh, Su-Jane Wang
Summary: This study found that Kaempferol 3-rhamnoside (KR) can inhibit 4-aminopyridine (4-AP)-evoked glutamate release. The inhibitory effect of KR is linked to the omission of external Ca2+ or the depletion of glutamate in synaptic vesicles, as well as the suppression of P/Q-type Ca2+ channel activity. Additionally, KR reduces the increase in Ca2+ concentration and the phosphorylation of CaMKII and its substrate synapsin I.
Article
Psychology, Biological
Ben Turley, Adam P. Swiercz, Laxmi Iyer, Paul J. Marvar
Summary: A customizable software program was developed for Ponemah to assess the internal physiological state of animals in real-time, providing valuable insights into mechanisms of neuropsychiatric diseases.
PHYSIOLOGY & BEHAVIOR
(2021)
Article
Biochemistry & Molecular Biology
Hamish Stevens-Bullmore, Don Kulasiri, Sandhya Samarasinghe
Summary: This paper mathematically models the activation of CaM by Ca2+ and the binding of CaM to downstream proteins. The study found that under high stimulation, CaM redistributes from low-affinity to high-affinity binding targets and represses activation of opposing CaM-binding proteins. The model also discovered that CaMKII can repress cAMP production by repressing CaM-regulated proteins. Under low levels of Ca2+ stimulation, CaM signaling is unstable and unlikely to induce synaptic depression alone.
Article
Biology
Lise M. Sjogaard-Frich, Andreas Prestel, Emilie S. Pedersen, Marc Severin, Kristian Kolby Kristensen, Johan G. Olsen, Birthe B. Kragelund, Stine Falsig Pedersen
Summary: Through various biophysical techniques, this study reveals the complex interaction mechanism between CaM and NHE1, identifying multiple states and structures of the NHE1:CaM complex that can be modulated by stoichiometries, calcium concentrations, and phosphorylation. Experimental results suggest that CaM may enhance NHE1 regulation and indicate that this structural diversity is relevant to other CaM complexes as well.
Article
Biochemistry & Molecular Biology
Andrew C. Hedman, Zhigang Li, Laetitia Gorisse, Swetha Parvathaneni, Chase J. Morgan, David B. Sacks
Summary: AMP-activated protein kinase (AMPK) is crucial in maintaining cellular energy homeostasis through regulating catabolic and anabolic pathways, with IQGAP1 identified as a novel regulator of AMPK signaling. Lack of IQGAP1 leads to impaired AMPK activation and metabolic dysregulation, highlighting the importance of the functional interplay between IQGAP1 and AMPK.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Pamela Gaitan-Gonzalez, Rommel Sanchez-Hernandez, Jose-Antonio Arias-Montano, Angelica Rueda
Summary: Metabolic syndrome is a pre-diabetic state characterized by insulin resistance, visceral fat accumulation, and dyslipidemias, which increase the risk for developing cardiovascular disease. Increased synergistic tone in metabolic syndrome may contribute to the onset of pre-diabetic cardiomyopathy. The alterations in protein kinase A and Ca2+/calmodulin-dependent protein kinase II play important roles in functional responses mediated by beta(1)-adrenoceptors in pre-diabetes.
WORLD JOURNAL OF DIABETES
(2021)
Article
Biochemistry & Molecular Biology
Shuhang Dai, Elisa Venturini, Saveg Yadav, Xiaoxuan Lin, Dylan Clapp, Martin Steckiewicz, Angela M. Gocher-Demske, Arthur M. Edelman, D. Grahame Hardie
Summary: This study reveals the important role of CaMKK2 in mediating EGFR signaling in cancer cells, with regulation mechanisms involving both calcium-dependent and calcium-independent pathways. Additionally, CaMKK2 regulates transcription of key genes, impacting cell survival and proliferation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Cell Biology
Shayahati Bieerkehazhi, Ying Fan, Savannah J. West, Ritika Tewari, Junsuk Ko, Tingting Mills, Darren Boehning, Askar M. Akimzhanov
Summary: The study demonstrates the importance of the enzyme DHHC21 in T cell receptor signal transduction and protein acylation. DHHC21 is critical for the activation of CD4(+) T cells and plays a role in the differentiation of effector T helper lineages.
JOURNAL OF CELL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Hiroshi Tokumitsu, Hiroyuki Sakagami
Summary: CaMKK acts as the activating kinase for multiple downstream kinases, playing important roles in various Ca2+-dependent pathways as well as pathophysiological pathways. Its molecular mechanism and pharmacological inhibitors are areas of focus in research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)