期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 8, 期 -, 页码 3587-3601出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S47665
关键词
thermosensitive microgels; ophthalmic drug delivery; hyaluronic acid; cyclosporine A
资金
- National Natural Science Foundation of China [81173006]
- Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University [JKGQ201107]
- Jiangsu Natural Science Foundation of China [SBK201322347]
- Qing Lan Project
- Jiangsu Overseas Research and Training Program for University Prominent Young and Middle-aged Teachers and Presidents
For nearly a decade, thermoresponsive ophthalmic in situ gels have been recognized as an interesting and promising ocular topical delivery vehicle for lipophilic drugs. In this study, a series of thermosensitive copolymers, hyaluronic acid-g-poly(N-isopropylacrylamide) (HA-g-PNIPAAm), was synthesized, by coupling carboxylic end-capped PNIPAAm to aminated hyaluronic acid through amide bond linkages, and was used as a potential carrier for the topical ocular administration of cyclosporine A (CyA). The lower critical solution temperature of HA-g-PNIPAAm(59) in aqueous solutions was measured as 32.7 degrees C, which was not significantly affected by the polymer concentration. Moreover, HA-g-PNIPAAm(59) microgels showed a high drug loading efficiency (73.92%) and a controlled release profile that are necessary for biomedical application. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) observations showed that HA-g-PNIPAAm microgels were spherical in shape with homogeneous size. Based on the result of the eye irritation test, the HA-g-PNIPAAm microgels formulation was shown to be safe and nonirritant for rabbit eyes. In addition, HA-g-PNIPAAm microgels achieved significantly higher CyA concentration levels in rabbit corneas (1455.8 ng/g of tissue) than both castor oil formulation and commercial CyA eye drops. Therefore, these newly described thermoresponsive HA-g-PNIPAAm microgels demonstrated attractive properties to serve as pharmaceutical delivery vehicles for a variety of ophthalmic applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据