Review
Biochemistry & Molecular Biology
Elena Adinolfi, Elena De Marchi, Marianna Grignolo, Bartosz Szymczak, Anna Pegoraro
Summary: The article provides an overview of the role of P2X7 purinergic receptor in the tumor microenvironment, including its activities in cancer proliferation, dissemination, and its interaction with immune and endothelial cells. The article also analyzes the release of microvesicles and exosomes, the content within them, and the impact of P2X7 activation on metastatic spread and niche conditioning. Furthermore, the article explores the emerging role of P2X7 in the adenosinergic axis and its influence on antitumor therapy responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Ping Li, Jie Wang, Mengqiu Gao, Jue Wang, Yi Ma, Yueqing Gu
Summary: Extracellular vesicles (EVs) have emerged as a promising tumor biomarker, offering benefits for cancer diagnosis, but the practicality of EV assays remains challenging due to various factors. A membrane-based biosensor has been developed for precise and sensitive EV identification, integrating EV capture and detection based on EV membrane features, showing great potential for clinical prostate cancer diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Review
Cell Biology
Zhenzhen Mo, Jia Yang Alex Cheong, Lirong Xiang, Minh T. N. Le, Andrew Grimson, Daniel Xin Zhang
Summary: Metastasis, the dissemination of primary tumor cells, is a major cause of cancer-related deaths. Metastatic organotropism, the specific distribution pattern of distant metastasis, is influenced by various factors. Extracellular vesicles (EVs) play a crucial role in organ-specific metastasis, carrying bioactive cargo that can modulate the pre-metastatic niche and influencing diagnostic and prognostic values. Counteracting EV functions could be a novel therapeutic strategy to target metastasis. More research is needed to understand the mechanisms of EVs in organ-specific metastasis for clinical translation.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Milene C. Vallejo, Soumyadeep Sarkar, Emily C. Elliott, Hayden R. Henry, Samantha M. Powell, Ivo Diaz Ludovico, Youngki You, Fei Huang, Samuel H. Payne, Sasanka Ramanadham, Emily K. Sims, Thomas O. Metz, Raghavendra G. Mirmira, Ernesto S. Nakayasu
Summary: Extracellular vesicles (EVs) have important roles in cell-to-cell communication and biomarker studies. In this study, a proteomics meta-analysis was performed to refine the composition of plasma EVs by separating EV proteins and contaminants into different clusters. The refined EV protein list obtained from this study provides a valuable resource for mechanistic and biomarker studies.
Review
Oncology
Antonia Schubert, Michael Boutros
Summary: Extracellular vesicles (EVs) have emerged as potential diagnostic and prognostic markers for cancer therapy, yet much remains unknown about their exact mechanisms of signaling specificity and cargo transfer. Several signaling cascades utilize EVs for signaling in tumor-stroma interaction, including key pathways such as Wnt and TGF-beta, which are tightly associated with tumor progression and metastasis. Multiple mechanisms of how EVs interplay with signaling cascades to mediate complex processes have been described, shedding light on the potential therapeutic applications and gaps in knowledge.
MOLECULAR ONCOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Jagannath Mondal, Shameer Pillarisetti, Vijayabhaskarreddy Junnuthula, Monochura Saha, Seung Rim Hwang, In-kyu Park, Yong-kyu Lee
Summary: Exosomes are natural nanocarriers secreted by cells, which can be modified to load additional cargo for expanded biomedical applications. They have the potential to overcome the limitations of conventional nanoparticle-based techniques in targeted therapy.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Nanoscience & Nanotechnology
Sijun Pan, Yan Zhang, Auginia Natalia, Carine Z. J. Lim, Nicholas R. Y. Ho, Balram Chowbay, Tze Ping Loh, John K. C. Tam, Huilin Shao
Summary: The platform developed in this study leverages circulating extracellular vesicles for activity-based assessment of tumour-specific drug-target interactions and can be used for clinical cancer treatment monitoring.
NATURE NANOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Andrew A. Lin, Hanfei Shen, Griffin Spychalski, Erica L. Carpenter, David Issadore
Summary: In this study, finite-element simulation and experimental characterization were used to elucidate design rules for isolating EV subpopulations from blood using TENPO chips. The utility of this approach was demonstrated by reducing device background without sacrificing target EV recovery. Comparison with gold-standard methods showed its wide application and modularity in targeting EV subpopulations from multiple disease models.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Pharmacology & Pharmacy
Hongwei Chen, Venkata Chengalvala, Hongxiang Hu, Duxin Sun
Summary: Nanomedicine refers to using nanoparticles to treat cancer, but recent research shows that cancer cells can also release nanoparticles to promote cancer metastasis, affecting the growth and survival of organisms.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biology
Wouter W. Woud, Edwin van der Pol, Erik Mul, Martin J. Hoogduijn, Carla C. Baan, Karin Boer, Ana Merino
Summary: This article presents a method to quantify and phenotype extracellular vesicles (EVs) in human plasma using imaging flow cytometry without prior isolation of EVs. The method eliminates selection bias or artifact introduced by EV isolation techniques and provides a framework for analyzing EVs in various experimental and potentially diagnostic settings.
COMMUNICATIONS BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Vivek P. Chavda, Anjali Pandya, Lalit Kumar, Nidhi Raval, Lalitkumar K. Vora, Sreeranjini Pulakkat, Vandana Patravale, Yanhong Duo, Ben Zhong Tang
Summary: This paragraph introduces the formation, structure, and function of exosomes, which play an important role in intercellular communication and are associated with health and disease. Recently, exosome-based nanotechnology has provided opportunities for the development of next-generation nanomedicines. This review summarizes the recent advances in exosome nanotechnology research, including classification, isolation and preparation, constitution, biological function, and nanobiomedical applications in disease treatment and diagnosis, and prospects for the future.
Review
Nanoscience & Nanotechnology
Nai Mu, Jie Li, Li Zeng, Juan You, Rong Li, Anquan Qin, Xueping Liu, Fang Yan, Zheng Zhou
Summary: This review provides an overview of plant-derived exosome-like nanovesicles (PELNs) and their potential implications for human health. It summarizes the roles of PELNs in respiratory, digestive, circulatory, and other diseases. The review also investigates the potential shortcomings and challenges in current research and proposes recommendations for future research directions.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Review
Oncology
Evelyn Lattmann, Mitchell P. Levesque
Summary: Melanoma, the deadliest form of skin cancer, can be diagnosed and treated using extracellular vesicles as biomarkers, which also reveal important signaling pathways for cancer progression.
Article
Multidisciplinary Sciences
V. M. Ukrainskaya, O. E. Musatova, D. V. Volkov, D. S. Osipova, D. S. Pershin, A. M. Moysenovich, E. G. Evtushenko, E. A. Kulakovskaya, E. G. Maksimov, H. Zhang, Y. P. Rubtsov, M. A. Maschan, A. V. Stepanov, A. G. Gabibov
Summary: Tumor-derived extracellular vesicles (EVs) play an active role in tumor metastasis and immunosuppression in the tumor microenvironment. These EVs can carry tumor surface molecules, such as tumor-associated antigens (TAAs) and/or checkpoint inhibitors, and potentially interact with T cells or CAR T cells. The interaction between EVs and CAR T cells can change the outcome of CAR T-based cancer immunotherapy and affect the function of CAR T cells. EVs can also serve as adjuvants and antigenic components in antitumor vaccines. Chronic exposure of CD19-CAR T cells to CD19(+)EVs leads to activation and systemic exhaustion in an antigen-specific manner, accompanied by impaired cytotoxic activity in vitro.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Roman Stukelj, Karin Schara, Apolonija Bedina-Zavec, Vid Sustar, Manca Pajnic, Ljubisa Paden, Judita Lea Krek, Veronika Kralj-Iglic, Anita Mrvar-Brecko, Rado Jansa
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Pharmacology & Pharmacy
K. Schara, R. Stukelj, J. L. Krek, K. Lakota, S. Sodin-Semrl, A. J. M. Boulton, V. Kralj-Iglic
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Chemistry, Multidisciplinary
Roghayeh Imani, Ralf Dillert, Detlef W. Bahnemann, Meysam Pazoki, Tomaz Apih, Veno Kononenko, Neza Repar, Veronika Kralj-Iglic, Gerrit Boschloo, Damjana Drobne, Tomas Edvinsson, Ales Iglic
Editorial Material
Orthopedics
Veronika Kralj-Iglic
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
(2018)
Article
Chemistry, Multidisciplinary
Jan Gimsa, Philip Wysotzki, Sarka Perutkova, Thomas Weihe, Patrick Elter, Piotr Marszalek, Veronika Kralj-Iglic, Torsten Mueller, Ales Iglic
Review
Chemistry, Medicinal
Ekaterina Gongadze, Luka Mesarec, Veronika Kralj-Iglic, Ales Iglic
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Matej Daniel, Jitka Reznickova, Milan Handl, Ales Iglic, Veronika Kralj-Iglic
SCIENTIFIC REPORTS
(2018)
Article
Physics, Multidisciplinary
Mitja Drab, Veronika Kralj-Iglic
Article
Veterinary Sciences
Metka Simundic, Tanja Svara, Roman Stukelj, Judita L. Krek, Mitja Gombac, Veronika Kralj-Iglic, Natasa Tozon
VETERINARY AND COMPARATIVE ONCOLOGY
(2019)
Article
Multidisciplinary Sciences
Veronika Kralj-Iglic, Gabriella Pocsfalvi, Luka Mesarec, Vid Sustar, Henry Hagerstrand, Ales Iglic
Review
Biochemistry & Molecular Biology
Jeel Raval, Ekaterina Gongadze, Metka Bencina, Ita Junkar, Niharika Rawat, Luka Mesarec, Veronika Kralj-Iglic, Wojciech Gozdz, Ales Iglic
Summary: This paper theoretically explains the origin and mechanism of electrostatic interactions between lipid bilayers and charged solid surfaces from the perspective of statistical mechanics, exploring the important relationship between the two and the influence of surface nanostructures. Through mathematical models and experimental results, it describes the morphology and influencing factors of lipid vesicle adsorption on solid surfaces.
Article
Biochemistry & Molecular Biology
Ekaterina Gongadze, Luka Mesarec, Samo Kralj, Veronika Kralj-Iglic, Ales Iglic
Summary: In this modified Langevin Poisson-Boltzmann model, an analytical expression for osmotic pressure between charged surfaces was derived, considering the orientational ordering of water dipoles and the spatial dependencies of electric potentials, fields, and osmotic pressure. The predicted repulsion between different surfaces can facilitate the fission of membrane daughter vesicles and undulated membrane protrusions with connected beads.
Article
Biochemistry & Molecular Biology
Anna Romolo, Zala Jan, Apolonija Bedina Zavec, Matic Kisovec, Vesna Arrigler, Vesna Spasovski, Marjetka Podobnik, Ales Iglic, Gabriella Pocsfalvi, Ksenija Kogej, Veronika Kralj-Iglic
Summary: This study used interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (R-h) of small cellular particles (SCPs) from different sources. The results showed that ILM was a convenient method for evaluating SCPs in blood preparations, indicating its potential use in population and clinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marko Jeran, Anna Romolo, Vesna Spasovski, Matej Hocevar, Urban Novak, Roman Stukelj, Vid Sustar, Matic Kisovec, Apolonija Bedina Zavec, Ksenija Kogej, Ales Iglic, Polonca Trebse, Veronika Kralj-Iglic
Summary: Small cellular particles (SCPs) were isolated and characterized from spruce needle homogenate. They were imaged by SEM and cryo TEM, assessed for their properties by ILM and FCM, and analyzed for their content using UV-vis spectroscopy and GC-MS. The results showed that the spruce needle homogenate contained vesicles and other small particles, with SCPs having a higher number density and smaller size. The SCPs also showed a decrease in phenolic content over time and terpene content was found in the pellet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Giovanna L. Liguori, Veronika Kralj-Iglic
Summary: Cancer metastasis is responsible for 90% of cancer deaths worldwide. Current therapeutic approaches have limited efficiency, and there is a need for new strategies. Extracellular vesicles (EVs) play a crucial role in cell-cell communication and have an impact on cancer cell migration and metastasis.