Article
Pharmacology & Pharmacy
Jose Antonio Lebron, Pilar Lopez-Cornejo, Elena Garcia-Dionisio, Pablo Huertas, Margarita Garcia-Calderon, Maria Luisa Moya, Francisco Jose Ostos, Manuel Lopez-Lopez
Summary: The tendency of surfactants to self-aggregate plays a key role in determining their efficiency for compacting nucleic acids, with 12NBr showing the most effectiveness as a DNA compacting agent. Lipoplexes of DOPE/12NBr/pEGFP-C1 were prepared and characterized, with investigations into their cytotoxicity and transfection efficiency.
Article
Pharmacology & Pharmacy
Camille Ayad, Pierre Libeau, Celine Lacroix-Gimon, Catherine Ladaviere, Bernard Verrier
Summary: The approval of two mRNA vaccines as urgent prophylactic treatments against Covid-19 made them a realistic alternative to conventional vaccination methods. The development of an innovative vector called LipoParticles (LP) demonstrated significant advantages in cellular transfection.
Article
Pharmacology & Pharmacy
Zeynep Burcu Akkus-Dagdeviren, Sema Arisoy, Julian David Friedl, Andrea Fuerst, Ahmad Saleh, Andreas Bernkop-Schnuerch
Summary: The aim of this study was to develop enzyme-activated charge-reversal lipid nanoparticles (LNPs) as novel gene delivery systems. The researchers successfully anchored a transfection promoter on the surfaces of LNPs to achieve high encapsulation and efficient gene delivery. The experiment results showed that the enzyme-triggered charge reversal of LNPs resulted in significantly increased transfection efficiency in human alveolar epithelial cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Nanoscience & Nanotechnology
Erica Quagliarini, Junbiao Wang, Serena Renzi, Lishan Cui, Luca Digiacomo, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Heinz Amenitsch, Laura Masuelli, Roberto Bei, Daniela Pozzi, Augusto Amici, Francesco Cardarelli, Cristina Marchini, Giulio Caracciolo
Summary: Lipid nanoparticles (LNPs) have shown promise as effective gene delivery vehicles, with a specific formulation (LNP15) demonstrating successful transfection and potential application in cancer vaccines.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Jennifer Lien, Tawni Bull, Richard W. Michelmore, Ting Guo
Summary: Fluorescence titration using magnetic nanoparticles (FTMN) is a rapid, inexpensive, and simple method for quantifying the amount of fluorophore-intercalated plasmid DNA on DNA attractive nanoparticles. By confirming the binding of propidium iodide (PI)-intercalated DNA to polyethylenimine (PEI)-coated monodisperse iron oxide magnetic nanoparticles (PEI-MNs), the FTMN method provides a way to determine the amount of DNA on MNs, the dissociation constant, and binding energy. This method is validated by utilizing quantitative polymerase chain reaction to measure the amount of free PI/DNA in solution.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Shuang Wu, Meiyan Liu, Xiang Hu, Chengxi He, Chunyan Zhao, Shuanglin Xiang, Youlin Zeng
Summary: The chemical and physical structure of cationic liposomes significantly affect their gene transfection efficiency; the design of novel cationic liposomes is guided by the structure-function relationship investigation; the study shows that different lipid structures play a crucial role in gene transfection efficiency.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Review
Pharmacology & Pharmacy
Sarwar Beg, Waleed H. Almalki, Fahmida Khatoon, Khalid S. Alharbi, Saad Alghamdi, Md Habban Akhter, Habibullah Khalilullah, Abdullah A. Baothman, Abdul Hafeez, Mahfoozur Rahman, Sohail Akhter, Hani Choudhry
Summary: Cancer vaccines, utilizing nucleic acid derivatives like plasmid DNA, small interfering RNA, and mRNA, can be personalized for patients, while nanomedicines have shown to be efficient drug carriers for delivering cancer vaccines. This review provides an overview of cancer vaccines literature, from their inception to current developments.
DRUG DISCOVERY TODAY
(2021)
Article
Chemistry, Physical
Dipanjan Mukherjee, Md Nur Hasan, Ria Ghosh, Gourab Ghosh, Arpan Bera, Sujanthi Easwara Prasad, Ankita Hiwale, Praveen K. Vemula, Ranjan Das, Samir Kumar Pal
Summary: Complexes of cationic liposomes with DNA have shown promise as nonviral vectors for gene therapy. This study examines the kinetics of liposome/DNA complex formation and reveals two different kinetic processes using novel cationic lipids. The observed difference in kinetics is likely due to variations in the lipid/DNA complex structures.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Medicine, Research & Experimental
Claudia Del Toro Runzer, Shivesh Anand, Carlos Mota, Lorenzo Moroni, Christian Plank, Martijn van Griensven, Elizabeth R. Balmayor
Summary: Nucleic acids have great potential for gene therapy, with plasmid DNA as the first therapeutic nucleic acid and mRNA as a safer and more affordable option. This study investigated the uptake mechanisms and efficiencies of genetic material by cells, focusing on different nucleic acids, delivery vectors, and human primary cells. The study found that caveolae-mediated uptake was the main route for gene delivery, pDNA had higher expression levels in fast-dividing fibroblasts, cmRNA was responsible for high protein production in slow-dividing osteoblasts, and in mesenchymal stem cells, the combination of vector and nucleic acid was more relevant. Protein expression was higher when cells were seeded on 3D scaffolds.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Engineering, Biomedical
Haimei Hu, Han Wang, Shuanghong Liang, Xiaoling Li, Dan Wang
Summary: Poly(amido-amine) (PAMAM) modified by hyaluronic acid (HA) showed reduced cytotoxicity and smaller particle size, with improved DNA delivering capacity in cancer cells line. The transfection efficiency of the modified polymers approached that of unmodified polyplexes, making them potential candidates for targeted gene delivery.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Nanoscience & Nanotechnology
Sandeep B. Shelar, Anusree Dey, Santosh L. Gawali, Saravanan Dhinakaran, Kanhu C. Barick, Manidipa Basu, Sheetal Uppal, Puthusserickal A. Hassan
Summary: Cationic liposomes formed in mixtures of lecithin and cationic amphiphiles can efficiently transfect plasmid DNA and siRNA into cells. By modulating the ratio of DDAB to phospholipid, the size of the vesicles can be adjusted, allowing for the formation of lipoplexes in cells and enhancing gene transfection efficiency.
ACS APPLIED BIO MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Vera Vysochinskaya, Sergey Shishlyannikov, Yana Zabrodskaya, Elena Shmendel, Sergey Klotchenko, Olga Dobrovolskaya, Nina Gavrilova, Darya Makarova, Marina Plotnikova, Ekaterina Elpaeva, Andrey Gorshkov, Dmitry Moshkoff, Mikhail Maslov, Andrey Vasin
Summary: The design of cationic liposomes composed of 2X3 and DOPE can greatly enhance mRNA delivery for mRNA-based therapies. The study investigated the effect of 2X3-DOPE composition and lipid-to-mRNA ratio (N/P) on mRNA transfection. It was found that lipoplex-mediated transfection efficiency relies on both liposome composition and N/P ratio, with liposomes 2X3-DOPE (1:3) showing superior delivery compared to Lipofectamine MessengerMax.
Article
Chemistry, Multidisciplinary
Daniel Hachim, Juzhi Zhao, Jash Bhankharia, Raquel Nunez-Toldra, Liliana Brito, Hyejeong Seong, Michele Becce, Liliang Ouyang, Christopher L. Grigsby, Stuart G. Higgins, Cesare M. Terracciano, Molly M. Stevens
Summary: Non-viral vectors provide safe and versatile options for nucleic acid delivery. In this study, nanoneedles were synergistically combined with nanofilms to achieve enhanced transfection efficiency compared to polyplexes in suspension. The results also showed that polysaccharide-based polyanions enhanced transfection efficiency, contrary to previous suggestions that only hyaluronic acid is effective. Additionally, the interaction with the CD44 receptor was found to not play a major role in the enhanced transfection.
Article
Pharmacology & Pharmacy
Carla Caddeo, Morena Gabriele, Amparo Nacher, Xavier Fernandez-Busquets, Donatella Valenti, Anna Maria Fadda, Laura Pucci, Maria Manconi
Summary: The study developed Eudragit-coated liposomes for safe transportation of resveratrol and artemisinin through the gastrointestinal tract to target the intestine, exhibiting anti-tumor effects. Results showed that these liposomes could induce significant cell death in intestinal adenocarcinoma cells, thereby inhibiting intestinal tumor growth.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Chemistry, Multidisciplinary
Jeongsoo Hur, Aram J. Chung
Summary: This review provides an overview of recent advances in microfluidic and nanofluidic intracellular delivery strategies and discusses new opportunities and challenges for clinical applications. Microfluidic and nanofluidic approaches as emerging solutions have shown remarkable potential for addressing this open challenge.
Review
Pharmacology & Pharmacy
Supusson Pengnam, Samarwadee Plianwong, Boon-ek Yingyongnarongkul, Prasopchai Patrojanasophon, Praneet Opanasopit
Summary: siRNA therapy can increase the deposition of chemotherapeutic drugs at the tumor site by suppressing the expression of multidrug-resistant genes. Nonviral delivery systems such as nanovesicles can improve siRNA delivery and have shown great potential in cancer therapy.
DRUG METABOLISM AND PHARMACOKINETICS
(2022)
Article
Engineering, Biomedical
Wipada Samprasit, Praneet Opanasopit, Benchawan Chamsai
Summary: The study developed dual-drugs-loaded nanoparticles for colon-specific delivery and synergistic activity against colon cancer cells. The nanoparticles showed enhanced activity and efficiency when both drugs were loaded, indicating potential for targeted nanomedicines in colon cancer treatment.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Pharmacology & Pharmacy
Taraswi Mitra Ghosh, Teeratas Kansom, Suman Mazumder, Joshua Davis, Ahmed S. Alnaim, Shanese L. Jasper, Chu Zhang, Aedan Bird, Praneet Opanasopit, Amit K. Mitra, Robert D. Arnold
Summary: The study found that the andrographolide analog 3A.1 has anti-tumor activity against mCRPC, either alone or in combination with taxanes, showing synergistic effects. Co-treatment of 3A.1 with taxanes reduced the required dose and regulated key factors such as heat shock proteins and MAT2A to exert its effects.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
Yin Yin Myat, Tanasait Ngawhirunpat, Theerasak Rojanarata, Praneet Opanasopit, Mark Bradley, Prasopchai Patrojanasophon, Chaiyakarn Pornpitchanarong
Summary: This study synthesized PEGDA/AA nanoparticles for the delivery of Dox to colorectal cancer cells. The nanoparticles demonstrated good physical properties, drug loading and release capabilities, non-toxicity to intestinal epithelial cells, and effective killing of colorectal cancer cells. These nanoparticles show potential as a drug delivery carrier for targeted, staged-release therapy for colon cancer.
Article
Pharmacology & Pharmacy
Phuvamin Suriyaamporn, Praneet Opanasopit, Worranan Rangsimawong, Tanasait Ngawhirunpat
Summary: This study designed and developed optimal two-layered dissolving microneedles (MNs) loaded with fluconazole-microemulsions (MEs) for the potential treatment of fungal eye infection. The optimal formulation of MNs exhibited good physical properties, complete insertion, minimally invasive ocular tissue, and high stability, while showing significantly higher FLUZ permeation into the ocular tissue and providing potential antifungal activity.
Article
Pharmacology & Pharmacy
Supusson Pengnam, Purin Charoensuksai, Boon-ek Yingyongnarongkul, Rungnapha Saeeng, Hasan Uludag, Prasopchai Patrojanasophon, Praneet Opanasopit, Samarwadee Plianwong
Summary: Breast cancer is the second leading cause of cancer-related death in the US. This study developed a chitosan derivative-stabilized 3nAG nanosuspension and improved its efficacy in killing breast cancer cells by adding siRNA.
Article
Pharmacology & Pharmacy
Benchawan Chamsai, Sirima Soodvilai, Praneet Opanasopit, Wipada Samprasit
Summary: This study developed a topical film-forming spray for antiseptic application. By evaluating different polymers and solvents, optimal film-forming polymers and solvent systems were selected and their physicochemical properties were evaluated. The in vivo evaluation showed that the spray formed a clear and flexible film quickly with antibacterial activity. In vitro evaluations also demonstrated that the spray was non-toxic to cells and did not interfere with wound healing. Therefore, this spray could be a promising candidate for topical antiseptic application.
Article
Pharmacology & Pharmacy
Kritsanaporn Tansathien, Phuvamin Suriyaamporn, Tanasait Ngawhirunpat, Praneet Opanasopit, Worranan Rangsimawong
Summary: The study investigates the potential of bioactive placenta for skin regeneration and develops dissolving microneedles loaded with bioactive extract for dermal delivery. The placental extracts contain high amounts of proteins, growth factors, and amino acids. They are non-toxic to skin cells and promote the proliferation of fibroblast cells. GP-loaded DMNs show the highest bioactivity and can serve as a novel approach for skin regeneration.
Article
Pharmacology & Pharmacy
Kritsanaporn Tansathien, Tanasait Ngawhirunpat, Worranan Rangsimawong, Prasopchai Patrojanasophon, Praneet Opanasopit, Nopparat Nuntharatanapong
Summary: This study assessed the effects of porcine placenta extract-loaded nano-transdermal systems on skin repair and hair growth promotion. The results showed that the system had various beneficial effects, including promoting cell growth and aggregation, reducing intracellular ROS and inflammatory cytokines, and inhibiting lipoxygenase activity. In a human study, the system improved skin hydration and promoted hair growth.
Article
Pharmacology & Pharmacy
Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, S. Kevin Li, Praneet Opanasopit
Summary: The present study evaluated the effect of gold nanoparticles stabilized by chitosan (CS-AuNPs) and citrate ions (Ci-AuNPs) on skin permeation of sodium fluorescein (NaFI) and rhodamine b base (RhB). CS-AuNPs showed enhanced permeation of NaFI, superior to Ci-AuNPs, and this enhancement was likely due to improved delivery through the transepidermal pathway. However, CS-AuNPs and Ci-AuNPs had no significant effect on the permeability of the lipophilic molecule RhB. CS-AuNPs also exhibited no cytotoxicity towards human skin fibroblast cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Thapakorn Charoenying, Praneet Opanasopit, Tanasait Ngawhirunpat, Theerasak Rojanarata, Prasert Akkaramongkolporn, Prasopchai Patrojanasophon
Summary: This study aimed to use a 3D printer to fabricate a tablet-shaped floating device for controlling the release and floating time of domperidone tablets. The device was designed with a PVA pathway to provide a predictable floating time, and the air chamber was intentionally detached to allow elimination from the body. The optimal formulation achieved a floating time of 24 hours and a controlled drug release rate.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Benchawan Chamsai, Praneet Opanasopit, Wipada Samprasit
Summary: This study developed fast disintegrating dosage forms for oral insulin delivery by incorporating mucoadhesive thiolated chitosan (TCS)-based nano-particles (NPs). The results showed that the CD-insulin complexes were successfully encapsulated into the mucoadhesive NPs, which were promising for use in oral insulin delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Phuvamin Suriyaamporn, Chaiyakarn Pornpitchanarong, Boonnada Pamornpathomkul, Prasopchai Patrojanasophon, Theerasak Rojanarata, Praneet Opanasopit, Tanasait Ngawhirunpat
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Supusson Pengnam, Praneet Opanasopit, Theerasak Rojanarata, Boon-ek Yingyongnarongkul, Chopaka Thongbamrer, Samarwadee Plianwong
Summary: The challenge in HER2-overexpressing breast cancer therapy is to develop an effective target therapy. This study formulated lipid-based nanoparticles for delivering siRNA into HER2-overexpressing breast cancer cells. The dual-targeted therapy using Mcl-1 siRNA nioplex and trastuzumab showed synergistic effects in inhibiting cell growth and inducing apoptosis.
Review
Pharmacology & Pharmacy
Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, S. Kevin Li, Praneet Opanasopit
Summary: Transdermal drug delivery via the skin is a promising method, and gold nanoparticles (AuNPs) have emerged as a useful tool in this area. This review provides a comprehensive summary of the properties, synthesis, and applications of AuNPs in transdermal drug delivery.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
