期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 15, 期 5, 页码 8526-8538出版社
MDPI AG
DOI: 10.3390/ijms15058526
关键词
porcine preadipocyte; miR-199a-5p; proliferation; differentiation
资金
- National Natural Science Foundation of China [31072014]
- Major Projects for Genetically Modified Organisms Breeding [2014ZX08009-047B]
MicroRNAs (miRNAs), a class of small non-coding RNAs, have emerged as novel and potent regulators of adipogenesis. However, few miRNAs have been fully investigated in porcine adipogenesis, given the fact that pig is not only an apropos model of human obesity research, but also a staple meat source of human diet. In this study, we showed that miRNA-199a-5p is highly expressed in porcine subcutaneous fat deposits compared to several other tissue types and organs measured alongside. Overexpression of miR-199a-5p in porcine preadipocytes significantly promoted cell proliferation while attenuating the lipid deposition in porcine adipocytes. By target gene prediction and experimental validation, we demonstrated that caveolin-1 (Cav-1) may be a bona fide target of miR-199a-5p in porcine adipocytes, accounting for some of miR-199a-5p's functions. Taken together, our data established a role of miR-199a-5p in porcine preadipocyte proliferation and differentiation, which is at least partially played by downregulating Cav-1.
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