Article
Biotechnology & Applied Microbiology
Lei Sun, Yingguo Bai, Xiu Zhang, Cheng Zhou, Jie Zhang, Xiaoyun Su, Huiying Luo, Bin Yao, Yuan Wang, Tao Tu
Summary: Three recombinant GAD genes from Bacillus strains were identified and successfully expressed heterologously in Escherichia coli. Using the engineered E. coli strain as a whole-cell biocatalyst, the biosynthesis of GABA from L-Glu was achieved, showing potential for industrial production of GABA.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Fei Zhu, Sheng Hu, Weirui Zhao, Lehe Mei
Summary: This study presents a novel method using immobilized glutamate decarboxylase (GAD) for GABA synthesis. The immobilized GAD showed increased stability and improved substrate affinity, making it suitable for continuous-scale GABA biosynthesis.
Article
Chemistry, Applied
Yu Sun, Dayi Ji, Haile Ma, Xiumin Chen
Summary: This study aimed to uncover the mechanism of GABA enrichment in coffee leaves under ultrasound treatment. The results showed that ultrasound increased the activity of enzymes related to GABA synthesis and changed the microstructure of coffee leaves, accelerating the migration of glutamate into cells. Metabolomics analysis identified various metabolic pathways involved in GABA increase.
Article
Biotechnology & Applied Microbiology
Sion Ham, Hyun Jin Kim, Nara Shin, Jeong Hyeon Hwang, Suk Jin Oh, Jun Young Park, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: This study demonstrates the efficient and economical production of GABA by immobilization and continuous production in a small-scale reactor. By optimizing the conditions, over 95% conversion of 600 mM monosodium glutamate to GABA can be achieved within 3 hours, and the immobilized cells can be reused for up to 15 times. Furthermore, a continuous production system operating in a 14 mL scale reactor produced 165 g of GABA after 96 hours of continuous operation, after optimizing the buffer concentration, substrate concentration, and flow rate.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Food Science & Technology
Zhou Chen, Run Wang, Yanyin Song, Aijin Ma, Siting Li, Yingmin Jia
Summary: Gamma-aminobutyric acid (GABA) is a potent bioactive compound with many anti-hypertensive and anti-depressant activities. A novel pyridoxal-5-phosphate monohydrate-dependent GAD (LcGAD10s) was cloned from Latilactobacillus curvatus and expressed in E. coli. LcGAD10s exhibited high stability and efficiency in converting GABA.
Article
Agronomy
Xiao Liu, Hao Ma, Jing Liu, Donghe Liu, Chunlei Wang
Summary: Watercore is a physiological disorder in Rosaceae fruits that causes hypoxia stress and fruit decay. The key enzyme PpGAD2 plays an important role in GABA synthesis, and exogenous GABA can inhibit the content of ethanol, MDA, and H2O2. PpGAD2 is significantly induced in watercore fruit, and its activity is affected by PpWRKY53 and several CML genes.
Article
Biotechnology & Applied Microbiology
Hyo Jung Lim, Dong-Hyun Jung, Eui-Sang Cho, Myung-Ji Seo
Summary: This study reports the first Gad from Lc.garvieae species and provides important enzymatic information related to GABA biosynthesis in the Lactococcus genus.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chengzhen Yao, Feng Shi, Xiaoyuan Wang
Summary: A high GABA-producing strain of Corynebacterium glutamicum was constructed by chromosomal editing, without the need for antibiotics or inducers. The strategy of enhancing l-glutamic acid synthesis and optimizing the copy numbers of GABA-related genes resulted in increased GABA production.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Mohammad Reza Edalatian Dovom, Mohammad Bagher Habibi Najafi, Paria Rahnama Vosough, Neda Norouzi, Seyyed Javad Ebadi Nezhad, Baltasar Mayo
Summary: A total of 50 lactic acid bacteria isolates from Iranian traditional dairy products were screened for GABA production. Eight isolates showed strong GAD activity and two Lactococcus lactis strains (311 and 491) were the strongest GABA producers. Optimal conditions for GABA production by these strains were determined.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Wenli Liu, Huamin Li, Long Liu, Kanghee Ko, Incheol Kim
Summary: This study screened seven strains of LAB producing GABA from kimchi, with F109-MD3 showing the highest GABA-producing ability. The strains, especially F109-MD3, were able to produce GABA effectively even in high concentrations of MSG, and the GAD activity exhibited a broad pH range and higher optimal temperature.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Su Jeong Park, Dong Hyun Kim, Hye Jee Kang, Minhye Shin, Soo-Yeon Yang, Jungwoo Yang, Young Hoon Jung
Summary: In this study, a synthetic medium was used to efficiently produce high titer of GABA using Lactobacillus plantarum. The optimized medium composition resulted in the highest GABA production compared to previous studies. The use of yeast extract as a secondary substrate source also contributed to achieving a yield exceeding the theoretical maximum.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Plant Sciences
Qinling Wei, Keqin Xie, Hongfei Wang, Xingfeng Shao, Yingying Wei, Yi Chen, Shu Jiang, Mengze Cao, Jisuan Chen, Feng Xu
Summary: The study investigated the effect of fructose on the content and metabolic pathway of gamma-aminobutyric acid (GABA) in broccoli sprouts. The results showed that fructose treatment increased the fresh weight, GABA, and glutamate contents in sprouts and promoted the activity of glutamic acid decarboxylase (GAD) and the expressions of BoGAD1 and BoGAD2. Fructose treatment also inhibited the stem length of broccoli sprouts and enhanced abscisic acid (ABA) production. Additionally, fructose treatment stimulated the Ca2+, CaM contents, and BoCaM2 expression, as well as increased inositol trisphosphate (IP3) content and activated phosphatidylinositol-specific phospholipase C (PI-PLC) activity and BoPLC2 expression, contributing to Ca2+ influx into the cells.
Article
Microbiology
Divakar Dahiya, Jemima V. Manuel, Poonam Singh Nigam
Summary: This article summarizes the research on synthesizing GABA through biological processes, identifying lactic acid bacteria and certain fungi as effective producers. These strains require specific growth conditions and common supplements including monosodium glutamate and pyridoxal phosphate, as well as maintaining an acidic pH.
Article
Neurosciences
Hideki Miwa, Ken Kobayashi, Shinobu Hirai, Mitsuhiko Yamada, Masahiko Watanabe, Haruo Okado, Yuchio Yanagawa
Summary: The study reveals the critical role of GABA in establishing connections between basket cells and Purkinje cells in the cerebellum, and its association with motor coordination in mice.
Article
Biotechnology & Applied Microbiology
Lovely Mahawar, Aparna Pandey, Kesava Priyan Ramasamy, Sakshi Pandey, Sheo Mohan Prasad
Summary: This article provides an overview of the research progress on the synthesis and metabolic pathways of GABA in cyanobacteria, as well as the feasibility of enhancing GABA production and extraction methods by modifying external conditions and gene expression. In addition, it introduces the commercial applications of GABA in various industries.
JOURNAL OF APPLIED PHYCOLOGY
(2022)