Article
Chemistry, Multidisciplinary
Hari Kalathil Balakrishnan, Soo Min Lee, Ludovic F. Dumee, Egan H. Doeven, Richard Alexander, Dan Yuan, Rosanne M. Guijt
Summary: 3D printing is used to fabricate devices with micro-level features and through the use of nanoporous materials, porous membranes can be integrated into 3D printed devices. In this study, nanoporous membranes were fabricated using digital light projection (DLP) 3D printing and resin exchange. A functionally integrated device was then created through a simple, semi-automated manufacturing process.
Article
Chemistry, Analytical
Sena Ghayyem, Alexandre Barras, Farnoush Faridbod, Sabine Szunerits, Rabah Boukherroub
Summary: This study investigated the antimicrobial properties of PNPG-PEG NPs, which have high thermal transformation efficiency and can generate cytotoxic ROS in the NIR region. The results showed that NIR light irradiation effectively killed planktonic bacteria and destroyed bacterial biofilms. PNPG-PEG NPs are proven to be an effective nanoplatform for killing pathogenic bacteria.
Article
Infectious Diseases
Anamaria Babosan, Margaux Gaschet, Anaelle Muggeo, Thomas Jove, David Skurnik, Marie-Cecile Ploy, Christophe de Champs, Fany Reffuveille, Thomas Guillard
Summary: Bacteria within biofilms exposed to sub-MIC concentrations of antibiotics can develop antibiotic resistance. This study shows that sub-MIC levels of aminoglycosides induce the SOS response in E. coli carrying the qnrD gene, leading to genetic rearrangements and increased biofilm formation. The presence of the qnrD-plasmid promotes biofilm production and the acquisition and spread of resistance determinants for other antibiotics.
Article
Infectious Diseases
Weidong Qian, Xinchen Li, Min Yang, Chanchan Liu, Yi Kong, Yongdong Li, Ting Wang, Qian Zhang
Summary: This study investigated the association between biofilm formation and antibiotic resistance in extensively drug-resistant Escherichia coli isolates. The results showed that stronger biofilm formation was correlated with larger proportions of extensively drug-resistant isolates. These findings provide novel insights for the prevention and treatment of E. coli-related infections.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Weidong Qian, Xinchen Li, Min Yang, Chanchan Liu, Yi Kong, Yongdong Li, Ting Wang, Qian Zhang
Summary: This study evaluated the antibiotic resistance/susceptibility profiles and biofilm-forming capacities of 81 Escherichia coli isolates from pediatric individuals in China, revealing an association between biofilm formation and resistance to several antibiotics for extensively drug-resistant strains. This finding provides novel insights for the prevention and treatment against E. coli-related infections.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biotechnology & Applied Microbiology
Shiju Abraham, Yair Kaufman, Francois Perreault, Ry Young, Edo Bar-Zeev
Summary: This study used atomic force microscopy to investigate the impact of phage T4 on the nanotopography and biomechanics of biofilm-forming E. coli cells. The results revealed that anchored cells undergoing biofilm formation experience a more gradual lysis with distinct nanoscale lesions, unlike the explosive lysis seen in planktonic cells. Furthermore, envelope rigidity and cell elasticity decrease significantly following T4 infection, indicating potential changes in the nanostructure of infected cells.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Immunology
Po-Cheng Tang, Olle Eriksson, Josefin Sjogren, Nikos Fatsis-Kavalopoulos, Johan Kreuger, Dan I. Andersson
Summary: Biofilms are a crucial mode of growth for bacteria, but how antibiotic resistance emerges and is selected in biofilms is poorly understood. This study developed and validated a microfluidic chip (Brimor) for studying the dynamics of antibiotic-resistant bacteria in biofilms. The chip was found to be easy to use and a relevant model for studying the selection of antibiotic resistance in bacterial biofilms, providing valuable insights into this area of research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Elina Tukia, Isa Hallman, Maarit Penttilae, Satu Haenninen, Maria Kareskoski
Summary: Endometritis is a common cause of infertility in mares, often caused by bacteria such as E. coli and beta-haemolytic streptococci. This study aimed to detect the presence and localization of E. coli bacteria in equine endometrial biopsies using a chromogenic RNAscope method. However, no evidence of bacterial invasion was found, regardless of the inflammatory status or previous bacterial culture results. Further research is needed to investigate potential localized infections or biofilm coverings that may have led to undetected bacterial presence.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Liming Jiang, Yaxian Jiang, Wen Liu, Rui Zheng, Chenghua Li
Summary: Escherichia coli, a gram-negative bacterium, is widely distributed and poses a threat to human and animal health. This study identified two phages with good antibiofilm effects against E. coli, which can be potential candidates for phage therapy.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Jingru Zuo, Lianjie Liu, Simeng Hou, Xiaoqiang Liu, Jiang Teng, Pei Li, Xiaotong Liu
Summary: Isoorientin exhibits significant antimicrobial and antibiofilm activity against carbapenem non-sensitive E. coli, making it a promising candidate for preventing food contamination and spoilage.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Simeng Hou, Jingjing Guo, Lianjie Liu, Fang Qiu, Xiaoqiang Liu
Summary: This study found that Lagotis brachystachya extract has moderate antibacterial activity against ESBLs-producing E. coli and can inhibit and eradicate biofilms produced by these bacteria.
Article
Environmental Sciences
Anne Mette Madsen, John Kerr White, Jeppe Lund Nielsen, Mehmet Emin Keskin, Kira Tendal, Margit Wagtberg Frederiksen
Summary: Breeding pigeons is associated with respiratory symptoms, as exposure to bacteria and endotoxin in pigeon coops may contribute to airway issues among breeders. The airborne dust in pigeon coops contained a diverse range of bacterial species, some of which could pose potential health risks.
ENVIRONMENTAL RESEARCH
(2022)
Review
Immunology
Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini
Summary: This study evaluated the correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli (UPEC) isolated from patients with urinary tract infections (UTI) globally. The results showed a significant positive correlation between biofilm formation and antibiotic resistance.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Melany Sanchez, Sara Soto
Summary: This study characterized 376 extraintestinal pathogenic E. coli strains collected from hospitals in Catalonia, Spain, between 2016 and 2017. The strains showed high resistance to several antibiotics and varied biofilm formation ability. Various virulence and antimicrobial resistance genes were detected, including the colibactin-encoding genes associated with colorectal cancer. The study also investigated the relationship between colibactin and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Divagar Muthukumar, Giorgi Shtenberg
Summary: A silver decorated porous silicon (Ag-pSi) surface-enhanced Raman scattering (SERS) platform was designed for rapid and reliable detection of Escherichia coli in various milk samples. The platform was optimized to achieve a high SERS effect and residual unreacted antibodies were evaluated using an indirect immunoassay. The platform showed high sensitivity and linear response for bacteria detection, and its selectivity and specificity were cross-validated. The applicability of the platform was confirmed in different milk samples and it showed high reliability, speed, and accuracy for on-site analysis.
Article
Biochemistry & Molecular Biology
Xiangke Duan, Yanrong Pan, Zhao Cai, Yumei Liu, Yingdan Zhang, Moxiao Liu, Yang Liu, Ke Wang, Lianhui Zhang, Liang Yang
Summary: This study demonstrated that antibiotic treatment of biofilm-related P. aeruginosa infections could induce a hyperbiofilm phenotype through rpoS mutation, possibly contributing to the failure of antimicrobial treatment in these infections. The evolved hyperbiofilm mutants outcompeted the ancestral strain within biofilms but not in planktonic cultures, and the mutation in rpoS gene increased intracellular c-di-GMP level and virulence in the hyperbiofilm variants.
CELL AND BIOSCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Bjarke H. Pedersen, Nicolas Gurdo, Helle Krogh Johansen, Soren Molin, Pablo I. Nikel, Ruggero La Rosa
Summary: A new high-throughput method for analyzing bacterial metabolic footprints has demonstrated a certain level of robustness and flexibility across different conditions, contributing to a better understanding of bacterial metabolism.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Microbiology
Angela Sandri, Janus Anders Juul Haagensen, Laura Veschetti, Helle Krogh Johansen, Soren Molin, Giovanni Malerba, Caterina Signoretto, Marzia Boaretti, Maria M. Lleo
Summary: In patients with cystic fibrosis, the main pathogen Pseudomonas aeruginosa often coexists with other microbes in the lungs, potentially affecting clinical outcomes. While some Achromobacter spp. isolates can inhibit P. aeruginosa biofilm formation, different strains of Achromobacter spp. may exhibit either competition or cohabitation with co-isolated P. aeruginosa strains during long-term co-infections. Genetic variants supporting higher virulence were found in a competitive Achromobacter sp. isolate, indicating the possible evolution of interplays during chronic infections.
Article
Biochemistry & Molecular Biology
Jiuxin Qu, Zhao Cai, Xiangke Duan, Han Zhang, Hang Cheng, Shuhong Han, Kaiwei Yu, Zhaofang Jiang, Yingdan Zhang, Yang Liu, Fang Bai, Yingxia Liu, Lei Liu, Liang Yang
Summary: Our study suggests that SARS-CoV-2 infection can lead to genetic adaptation of bacterial pathogens, promoting chronic infections. The adapted bacteria exhibit characteristics such as excessive biofilm formation and reduced virulence, enabling them to evade immune clearance. This highlights the importance of understanding the lung microenvironment post-virus infections and superinfection mechanism in COVID-19 patients.
CELL AND BIOSCIENCE
(2022)
Article
Chemistry, Medicinal
Jing Wang, Xi Liu, Kaiwei Yu, Moxiao Liu, Jiuxin Qu, Yingxia Liu, Zhao Cai, Ke Wang, Chao Zhuo, Liang Yang, Yingdan Zhang
Summary: The co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) in ICU patients is common but challenging to eliminate. Research suggests that antibiotic exposure reshapes the community compositions of dual-species biofilms, with different EPS structures playing roles in maintaining stability, highlighting the potential of targeting EPS structural fibers like Psi and extracellular DNA (eDNA) as a strategy for controlling polymicrobial biofilm-related infections.
ACS INFECTIOUS DISEASES
(2022)
Review
Biotechnology & Applied Microbiology
Xiaobo Liu, Bin Cao, Liang Yang, Ji-Dong Gu
Summary: Biofilm formation and biofilm-induced biodeterioration have significant impacts on the community. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a key signaling molecule in bacteria, regulating various bacterial processes. Understanding the regulatory mechanisms of c-di-GMP would benefit the control of bacterial processes and the development of anti-biofilm technologies.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Ecology
Adela M. Lujan, Steve Paterson, Elze Hesse, Lea M. Sommer, Rasmus L. Marvig, M. D. Sharma, Ellinor O. Alseth, Oana Ciofu, Andrea M. Smania, Soren Molin, Helle Krogh Johansen, Angus Buckling
Summary: Co-infecting pathogens can influence the selection of Pseudomonas aeruginosa mutators, limiting their fixation. This study is important for understanding bacterial evolution in chronic infections.
NATURE ECOLOGY & EVOLUTION
(2022)
Article
Infectious Diseases
Ruggero La Rosa, Helle Krogh Johansen, Soren Molin
Summary: Antibiotic resistance is predicted to be the biggest threat to human health by 2050, but we often overlook the difficulty in diagnosing and treating many bacterial infections. Persistent infections caused by opportunistic bacteria are becoming more successful in immunocompromised patients, even when the bacteria are susceptible to antibiotics. The mechanisms of persistence are complex and challenging to diagnose and treat.
Article
Biotechnology & Applied Microbiology
Yu-Ming Cai, Kai-Wei Yu, Ji-Hong Liu, Zhao Cai, Zun-Hao Zhou, Yang Liu, Tian-Fu Wang, Liang Yang
Summary: The c-di-GMP signaling pathways in P. aeruginosa are highly organized and play important roles in the transition between motile and sessile lifestyles. This study identified a phosphodiesterase, PipA, that regulates bacteriophage production, and uncovered a potential signaling circuit for biofilm treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Lise Goltermann, Kasper Langebjerg Andersen, Helle Krogh Johansen, Soren Molin, Ruggero La Rosa
Summary: A new mechanism of macrolide resistance caused by ribosomal protein mutations in Pseudomonas aeruginosa was investigated in this study. The results showed that macrolide antibiotics are still effective against P. aeruginosa and resistance development should be considered in patients receiving prolonged courses of macrolide treatment. Improved macrolide susceptibility testing is necessary for the detection of resistant bacteria.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Letter
Infectious Diseases
Lise Goltermann, Helle Krogh Johansen, Soren Molin, Ruggero La Rosa
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Claudia A. Colque, Andrea G. Albarracin Orio, Pablo E. Tomatis, Gina Dotta, Diego M. Moreno, Laura G. Hedemann, Rachel A. Hickman, Lea M. Sommer, Sofia Feliziani, Alejandro J. Moyano, Robert A. Bonomo, Helle K. Johansen, Soren Molin, Alejandro J. Vila, Andrea M. Smania
Summary: This study provides insights into the long-term adaptive evolution and resistance development of Pseudomonas aeruginosa in the airways of a cystic fibrosis patient. The findings highlight the role of mutations in the chromosomally encoded class C beta-lactamase gene in shaping antibiotic resistance and reveal the impact of prolonged antibiotic treatment on the evolution of multidrug-resistant phenotypes. Understanding the molecular mechanisms of resistance in natural environmental settings is crucial for addressing the challenge of antimicrobial resistance.
Article
Microbiology
Tianyuan Jia, Dongjing Liu, Xianbiao Bi, Menglu Li, Zhao Cai, Jiapeng Fu, Zhi Liu, Pengyao Wu, Xue Ke, Aiqun Jia, Guoliang Zhang, Guobao Li, Liang Yang
Summary: This study reveals that the AhR ligand Pht and its vitamin K analogs can inhibit the quorum sensing mechanism of P. aeruginosa and activate immune-modulating functions, suggesting their potential as alternative treatments for infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Mingxing Tang, Shumin Liao, Jing Qu, Yixin Liu, Shuhong Han, Zhao Cai, Yunping Fan, Liang Yang, Shuo Li, Liang Li
Summary: This study used human airway organoids (HAOs) as a host model to investigate Pseudomonas aeruginosa biofilm development. Dual transcriptome sequencing revealed that quorum sensing (QS) was the most dominant virulence pathway, profoundly affecting both bacterial biofilm and host immune responses. The study demonstrated that HAOs are an optimal model for studying the interaction between the airway epithelium and bacterial pathogens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Guohui Xiao, Zhao Cai, Qinglong Guo, Taosheng Ye, Yimin Tang, Peikun Guan, Juanjuan Zhang, Min Ou, Xiangdong Fu, Lili Ren, Minfei Yu, Zhaoqin Wang, Lei Liu, Liang Yang, Guoliang Zhang
Summary: This study aimed to determine the lung microbiota profile associated with pulmonary tuberculosis (TB) and characterize the changes during anti-TB treatment. The lung microbiota of untreated pulmonary TB patients was found to be distinct from that of healthy individuals and lung cancer patients. Anti-TB treatment significantly affected the diversity of the lung microbiota and induced the presence of antibiotic resistance genes.
MICROBIOLOGY SPECTRUM
(2022)
