Review
Cell Biology
Yonghong Luo, Haocheng Lu, Daoquan Peng, Xiangbo Ruan, Yuqing Eugene Chen, Yanhong Guo
Summary: Liver-humanized mice, established by reconstituting mouse liver with human hepatocytes, have emerged as an attractive animal model to study drug metabolism and evaluate therapeutic effects in human liver due to their ability to replicate enzymatic features of human hepatocytes. However, the uncertain replication of metabolic profiles compared to humans limits their application in studying metabolic disorders.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Orthopedics
B. Bodic, M. A. Boutet, C. Boyer, B. Metayer, C. Vignes, J. Lesoeur, J. Veziers, V. Daguin, F. Haspot, Y. Maugars, F. Djouad, J. Guicheux, C. Vinatier
Summary: This study demonstrated the feasibility of inducing osteoarthritis (OA) in immunologically humanized mice by destabilization of the medial meniscus (DMM). The developed OA model could serve as a useful tool for bridging the gap between preclinical and clinical evaluation of immune interacting disease-modifying osteoarthritis drugs (DMOADs) and cell-based therapies.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Biochemistry & Molecular Biology
Natalia Vieira Segatto, Camila Bonemann Bender, Fabiana Kommling Seixas, Kyle Schachtschneider, Lawrence Schook, Noah Robertson, Aisha Qazi, Maximillian Carlino, Luke Jordan, Courtni Bolt, Tiago Collares
Summary: Improving current therapy options and identifying more effective treatments for bladder cancer is critical, and the development of biological models capable of recapitulating the characteristics of human bladder cancer is essential for evaluating the effectiveness of new therapies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Shotaro Uehara, Yuichi Iida, Miyuki Ida-Tanaka, Motohito Goto, Kenji Kawai, Masafumi Yamamoto, Yuichiro Higuchi, Satoshi Ito, Riichi Takahashi, Hidetaka Kamimura, Mamoru Ito, Hiroshi Yamazaki, Mitsuo Oshimura, Yasuhiro Kazuki, Hiroshi Suemizu
Summary: This study developed a novel humanized liver mouse model that can accurately evaluate human drug metabolism without interference from mouse hepatocytes.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mengya Zhang, Xin Wang, Qiuchen Liu, Yelian Yan, Changzhi Xu, Zhihua Zhu, Sucheng Wu, Xiaoyu Zuo, Yanfeng Zong, Chunxiao Li, Yunhai Zhang, Zubing Cao
Summary: 3-MC exposure has toxic effects on the maturation of oocytes and development of embryos. It inhibits cumulus expansion and polar body extrusion, reduces cleavage and blastocyst rates, induces spindle abnormalities and chromosomal misalignments, and alters the levels of mitochondria, cortical granules, acetylated alpha-Tubulin, reactive oxygen species, DNA damage, and apoptosis. Abnormal gene expression related to cumulus expansion and apoptosis is also observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Carolina Rojas, Michelle P. Garcia, Alan F. Polanco, Luis Gonzalez-Osuna, Alfredo Sierra-Cristancho, Samanta Melgar-Rodriguez, Emilio A. Cafferata, Rolando Vernal
Summary: Periodontitis is an oral inflammatory disease caused by polymicrobial synergy and dysbiosis, leading to breakdown of tooth-supporting tissues and tooth loss. Human studies are ethically limited, but animal models can partially emulate the pathogenesis of periodontitis; humanized animal models are able to mimic human-like immune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Renyun Tian, Di Yang, Biaoming Xu, Rilin Deng, Binbin Xue, Luoling Wang, Huiyi Li, Qian Liu, Xiaohong Wang, Songqing Tang, Mengyu Wan, Hua Pei, Haizhen Zhu
Summary: This research established a hepatocellular carcinoma cell line, HLCZ01, that can support the complete life cycle of HBV. Comparisons with other cell lines and the analysis of gene expression profiles allowed exploration of factors affecting virus infection efficiency. Additionally, an experimental platform for human liver chimeric mice was developed. These models provide valuable tools for studying the mechanisms of HBV infection and the treatment of chronic hepatitis B.
MICROBIOLOGY SPECTRUM
(2023)
Review
Physiology
Yu-long Bao, Li Wang, Hai-ting Pan, Tai-ran Zhang, Ya-hong Chen, Shan-jing Xu, Xin-li Mao, Shao-wei Li
Summary: Liver fibrosis is a crucial process in chronic liver disease progression, with significant impact on prognosis. Research on liver fibrosis involves assessing different models and characteristics, providing essential insights into disease mechanisms and therapeutic approaches.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Environmental Sciences
Qihao Xu, Lingyu Hu, Siqi Chen, Xiaoyong Fu, Ping Gong, Zeyao Huang, Wenyu Miao, Cuiyuan Jin, Yuanxiang Jin
Summary: Exposure to 3-methylcholanthrene (3 MC) in maternal mice was found to have potential impacts on offspring, affecting bile acid metabolism and gut barrier function, with females being more seriously affected.
Review
Instruments & Instrumentation
Prakash Khadka, Jack Dummer, Philip C. Hill, Rajesh Katare, Shyamal C. Das
Summary: Inhaled drug delivery is a promising method for treating tuberculosis and reducing treatment duration. This review examines the clinical implications of pulmonary drug delivery in TB treatment, as well as the delivery systems and animal studies involving rifampicin. The lack of relevant clinical studies and high-dose animal models for inhaled rifampicin formulations is identified as a research gap.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Kat Kessler, Andreas Giannisis, Greg Bial, Lander Foquet, Henrietta M. Nielsen, Jacob Raber
Summary: This study compared the behavioral and cognitive performance of FRGN mice with humanized liver and mice with human apoE3 production throughout the body, and found that hepatic apoE may be implicated in behavioral and cognitive performance.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Devaraj Venkatapura Chandrashekar, Barent N. DuBois, Mamunur Rashid, Reza Mehvar
Summary: Chronic cirrhosis induced by 10 weeks of intraperitoneal TAA in rats leads to significant reductions in P450 enzyme proteins and activities, which are independent of the direct inhibitory effects of TAA.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Immunology
Paula Martinez-Sanz, Adrien R. G. Laurent, Edith Slot, Mark Hoogenboezem, Nikolina Babala, Robin van Bruggen, Anthony Rongvaux, Richard A. Flavell, Godelieve A. M. Tytgat, Katka Franke, Hanke L. Matlung, Taco W. Kuijpers, Derk Amsen, Julien J. Karrich
Summary: In this study, the researchers established a model using humanized MISTRG mice to study the biology and contribution of human neutrophils in the immune system. They found that these neutrophils possessed normal functional properties and could be released from the bone marrow in response to certain agents. The model also demonstrated the ability of human neutrophils to react to inflammation and infiltrate tumors.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jilong Ren, Dawei Yu, Jing Wang, Kai Xu, Yanan Xu, Renren Sun, Peipei An, Chongyang Li, Guihai Feng, Ying Zhang, Xiangpeng Dai, Hongye Zhao, Zhengzhu Wang, Zhiqiang Han, Haibo Zhu, Yuchun Ding, Xiaoyan You, Xueqin Liu, Meng Wu, Lin Luo, Ziyi Li, Yong-Guang Yang, Zheng Hu, Hong-Jiang Wei, Liangpeng Ge, Tang Hai, Wei Li
Summary: We successfully generated pig models with severe immunodeficiency that could construct human liver tissues.
CELL AND BIOSCIENCE
(2022)
Article
Immunology
Fabien Le Chevalier, Pierre Authie, Sebastien Chardenoux, Maryline Bourgine, Benjamin Vesin, Delphine Cussigh, Yohann Sassier, Ingrid Fert, Amandine Noirat, Kirill Nemirov, Francois Anna, Marion Berard, Francoise Guinet, David Hardy, Pierre Charneau, Francois Lemonnier, Francina Langa-Vives, Laleh Majlessi
Summary: This study generated new transgenic mouse strains with humanized ACE2 gene that display high permissiveness to the replication of SARS-CoV-2 Omicron sub-variants and were successfully used to evaluate the efficacy of a COVID-19 vaccine.
MICROBES AND INFECTION
(2023)