Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Yifang Cui, Fangfang Guo, Xuwang Cai, Xiaoya Cao, Jie Guo, Hongjun Wang, Bing Yang, Hongzhuan Zhou, Xia Su, Patrick J. Blackall, Fuzhou Xu
Summary: Glaesserella parasuis is the causative agent of Glasser's disease in swine. Molecular serotyping based on variation within the capsule loci is more accurate and efficient than traditional serological serotyping. The developed qPCR method showed high accuracy in distinguishing between G. parasuis serovars and was effective in clinical isolates as well.
VETERINARY MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
M. G. Rizzo, S. Carnazza, L. M. De Plano, D. Franco, M. S. Nicol, V Zammuto, S. Petralia, Giovanna Calabrese, C. Gugliandolo, S. Conoci, S. P. P. Guglielmino
Summary: The study developed a new method for rapid and sensitive detection of bacteria in blood using engineered M13 phages and PCR in a miniaturized silicon microchip, providing a fast and efficient diagnosis approach.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Genetics & Heredity
Sabina Maria Bruno, Giovanna Blacona, Stefania Lo Cicero, Germana Castelli, Mariarita Virgulti, Giancarlo Testino, Silvia Pierandrei, Andrea Fuso, Giuseppe Cimino, Giampiero Ferraguti, Adriana Eramo, Marco Lucarelli
Summary: In the precision medicine era of cystic fibrosis (CF), accurate quantification of CFTR gene expression is crucial for personalized therapeutic approaches. This paper compares two methods for quantifying CFTR gene expression and discusses their advantages and limitations.
Article
Agronomy
Stefania Fontanazza, Alessia Restuccia, Giovanni Mauromicale, Aurelio Scavo, Cristina Abbate
Summary: The study aimed to identify microorganisms responsible for degrading BP mulch films through a combination of biodegradation and molecular tests. A field trial revealed a higher abundance of Pseudomonas putida in BP plots, showing potential for accelerating the degradation of BP mulch films and reducing plastic pollution in agriculture.
Review
Biochemistry & Molecular Biology
Shuwen Lei, Song Chen, Qingping Zhong
Summary: Digital PCR (dPCR) has gained considerable attention for its ability to quantitate different types of pathogens with desired accuracy, sensitivity, and reproducibility, using end-point measurement for absolute quantification without the need for standard curves. It provides guidance for further genetic analysis and molecular diagnosis, contributing to the quantification of low level of nucleic acid and early detection of pathogenic diseases. The benefits, challenges, and future prospects of dPCR have been highlighted in this review, showcasing its potential as a powerful tool for pathogen detection and control.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Junxia Feng, Xiaohu Cui, Bing Du, Hanqing Zhao, Yanling Feng, Jinghua Cui, Chao Yan, Lin Gan, Zheng Fan, Tongtong Fu, Ziying Xu, Zihui Yu, Rui Zhang, Shuheng Du, Ziyan Tian, Qun Zhang, Guanhua Xue, Jing Yuan
Summary: This study developed a rapid and sensitive ddPCR assay for detecting Klebsiella pneumoniae in fecal samples. The ddPCR assay showed higher sensitivity, specificity, and lower inhibition compared to real-time PCR and microbial culture. The findings suggest that ddPCR could be an effective tool for detecting K. pneumoniae in fecal samples and guiding treatment decisions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Yi Huang, Shuqi Xiao, Zhiming Yuan
Summary: The study compared and evaluated the sensitivity, reproducibility, and specificity of 23 in-house assays for ebolavirus detection, finding that targeting the more conservative NP gene may offer better sensitivity for EVD case definition and quantitative measurement. This analysis could contribute to the standardization of ebolavirus detection and quantification assays, providing a better understanding of results and making implementation easier, particularly during outbreaks.
Article
Biochemistry & Molecular Biology
Ravikumar Harshitha, Duraipandian Rex Arunraj
Summary: Real-time quantitative PCR is a technique used for monitoring the PCR reaction in real time, classified into absolute and relative quantification based on purpose. Absolute quantification is used in various fields like microbiology, food technology, and biotechnology, while relative quantification is used in genomics and functional transcriptomics for gene expression analysis.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2021)
Article
Hematology
Sanyun Wu, Yixuan Li, Ruiying Huang, Ting Li, Yalan Yu, Ping Luo, Xuelan Zuo, Li He
Summary: The early diagnosis and intervention of myeloproliferative neoplasm (MPN) are crucial for prognosis. This study compared the performance of real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) in detecting the burden of JAK2V617F mutation. The results showed that ddPCR outperformed qPCR in terms of accuracy, sensitivity, and stability, particularly in low burden cases.
ANNALS OF HEMATOLOGY
(2023)
Article
Microbiology
Agata Anna Cisek, Iwona Bak, Bozena Cukrowska
Summary: Methanogenic archaea are important but often ignored in studies of human and animal intestinal microbiota. This research focused on improving the quantification of methanogens using qPCR, by refining the protocol and increasing the specificity and sensitivity of the assay. The results showed increased detection range and successful validation parameters, allowing for improved detection and quantification of methanogens in stool samples, such as chicken droppings in this study.
Article
Food Science & Technology
Chenxi Huang, Binti Youssouf Mahboubat, Yifeng Ding, Qile Yang, Jia Wang, Min Zhou, Xiaohong Wang
Summary: The study developed a rapid and accurate detection method for Salmonella using the lytic Siphovirus phage LPST10, which showed a detection limit of <30 CFU/mL in different food matrices. The method involves magnetic separation and real-time PCR detection, allowing for quick and efficient detection of Salmonella in complex food samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Applied
Joana Costa, Caterina Villa, Liliana Grazina, Isabel Mafra
Summary: This study proposes two highly sensitive and specific single-tube nested real-time PCR methods for the analysis of cashew nut as an allergenic food. The method targeting the Ana o 3 gene showed the highest relative sensitivity in dough/biscuit and was successfully validated for precision and accuracy.
Review
Food Science & Technology
Parisa Shokryzadan, Mohamed Ali Rajion, Goh Yong Meng, Liang Juan Boo, Mahdi Ebrahimi, Maryam Royan, Mahbod Sahebi, Parisa Azizi, Rambod Abiri, Mohammad Faseleh Jahromi
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2017)
Article
Agriculture, Dairy & Animal Science
M. F. Jahromi, J. B. Liang, R. Ebrahimi, A. F. Soleimani, A. Rezaeizadeh, N. Abdullah, P. Shokryazdan
Article
Agriculture, Dairy & Animal Science
Saeid Jafari, Yong M. Goh, Mohamed A. Rajion, Mohammad F. Jahromi, Yusof H. Ahmad, Mahdi Ebrahimi
ANIMAL SCIENCE JOURNAL
(2017)
Article
Agriculture, Dairy & Animal Science
S. C. L. Candyrine, M. F. Jahromi, M. Ebrahimi, J. B. Liang, Y. M. Goh, N. Abdullah
ANIMAL PRODUCTION SCIENCE
(2017)
Article
Biotechnology & Applied Microbiology
Mohammd Faseleh Jahromi, Parisa Shokryazdan, Zulkifli Idrus, Rohollah Ebrahimi, Fatemeh Bashokouh, Juan Boo Liang
BIOMED RESEARCH INTERNATIONAL
(2017)
Article
Agriculture, Dairy & Animal Science
N. Hedayat-Evrigh, F. Moradi, V. Vahedi, B. Navidshad, A. Seifi-Jamadi
SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE
(2019)
Article
Food Science & Technology
Roghayeh Valizadeh Yonjalli, Farzad Mirzaei Aghjehgheshlagh, Ali Mahdavi, Bahman Navidshad, Hamid Staji
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mohammad Mohammadrezaei, Bahman Navidshad, Abbasali Gheisari, Mehdi Toghyani
Summary: The study aimed to evaluate the effects of bioactive peptides derived from enzymatic hydrolysis of cottonseed meal compared to zinc bacitracin on broiler chickens. Results showed that adding CSBP could improve growth rate and balance gut microbiota, suggesting it as a potential alternative to antibiotics in an AGP free production system.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Biophysics
Masoud Eskandani, Morteza Eskandani, Somayeh Vandghanooni, Bahman Navidshad, Farzad Mirzaei Aghjehgheshlagh, Ali Nobakht
Summary: This study successfully enhanced the bioavailability of L-carnitine (LC) using solid lipid nanoparticles (SLNs) as carriers and demonstrated the protective effects of LC-SLNs against intracellular oxidative stress damages.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Agriculture, Dairy & Animal Science
M. Hashemi, B. Navidshad, H. Lotfollahian, F. Mirzaei Aghjehgheshlagh, S. Karamati
Summary: This study investigated the effects of autoclaving and microwave radiation on the chemical composition, metabolic energy, and nutrient digestibility of wheat screening waste. The results showed that heat treatments decreased the waste's gross energy, with microwave treatment having the most significant effect. The heat treatments also caused notable changes in the waste's chemical composition.
IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
A. Biria, B. Navidshad, F. Mirzaei Aghjehgheshlag, S. Nikbin
IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE
(2020)
Article
Veterinary Sciences
Roghayeh Valizadeh Yonjalli, Farzad Mirzaei Aghjehgheshlagh, Ali Mahdavi, Bahman Navidshad, Hamid Staji
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
(2019)
Article
Agriculture, Dairy & Animal Science
S. Ghaderzadeh, F. Mirzaei Aghjegheshlagh, S. Nikbin, B. Navidshad
IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE
(2019)
Article
Agriculture, Dairy & Animal Science
Y. L. Gong, J. B. Liang, M. F. Jahromi, Y. B. Wu, A. G. Wright, X. D. Liao
Article
Biology
Parisa Shokryazdan, Mohammad Faseleh Jahromi, Salwani M. D. Saadand, Mahdi Ebrahimi, Zulkifli Idrus, Hailong Zhou, Xiao Ping Diao, Juan Boo Liang