Article
Chemistry, Applied
Laura Hanley, Saeed M. Ghazani, Alejandro G. Marangoni
Summary: The spontaneous formation of phospholipid vesicles was explored for the production of liposomal encapsulations of bioactive compounds. Stable vesicles were produced from unrefined soybean and sunflower lecithin, and their structure and encapsulation capacity were evaluated. These vesicles demonstrated high thermal and oxidative stability, making them suitable for food applications.
Article
Chemistry, Analytical
Tianyi Dou, Clara Zens, Katrin Schroeder, Yuan Jiang, Alexey A. Makarov, Stephan Kupfer, Dmitry Kurouski
Summary: Liposomes are a promising therapeutic formulation for targeted drug delivery. AFM-IR analysis can be used to characterize the structure and morphology of liposomes at the single-vesicle level, as well as to investigate the conformational changes in phospholipids during liposome assembly. Additionally, liposomes show great potential for protein internalization.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Gabriela Angeles-Robles, Luis Carlos Ortiz-Dosal, H. Aranda-Espinoza, Vanesa Olivares-Illana, Jose Luis Arauz-Lara, S. Aranda-Espinoza
Summary: This study investigates the effect of actin proteins on the mechanical properties of GUVs. It is found that polymerized actin makes the vesicles more rigid and resistant to deformations.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Chemistry, Analytical
Tianyi Dou, Clara Zens, Katrin Schroder, Yuan Jiang, Alexey A. Makarov, Stephan Kupfer, Dmitry Kurouski
Summary: Liposomes are promising therapeutic formulations for targeted drug delivery. AFM-IR is a powerful technique that can be used to characterize the structural and morphological changes of lipids in liposomes. The study also demonstrates the rapid internalization of proteins by liposomes using melittin as a test protein. This research provides new insights into the molecular mechanisms of liposome-drug interactions.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Carina Dargel, Friederike Graebitz-Braeuer, Ramsia Geisler, Pascal Fandrich, Yvonne Hannappel, Lionel Porcar, Thomas Hellweg
Summary: This study investigates the role of charged lipids in the plasma membrane, particularly in relation to their interaction with the antiviral saponin glycyrrhizin. The findings reveal complete miscibility of DOPG and glycyrrhizin in small unilamellar vesicles, even at a ratio of 1:1. Additional information about the chain-chain correlation distance in the SUV structures was obtained through SANS, SAXS, and WAXS techniques.
Article
Chemistry, Multidisciplinary
Irene Abelenda-Nunez, Francisco Ortega, Ramon G. Rubio, Eduardo Guzman
Summary: This study aims to investigate the diffusion of biological macromolecules in the cytoplasm, where there is a strict limitation on the accessible volume. By studying the thermal motion of spherical colloidal beads in giant unilamellar vesicles (GUVs), it is found that confinement and environmental viscoelasticity result in anomalous motion pathways for the encapsulated microbeads. This anomalous diffusion is dependent on the ratio between the particle and GUV volume, as well as the viscosity of the particle's liquid environment.
Article
Chemistry, Physical
Ricki Chairil, Noah Malmstadt
Summary: Curvature-mediated lipid-protein interactions play a crucial role in cellular reactions and mechanisms. This study investigates the membrane curvature partitioning behavior of cube-shaped CsPbBr3 quantum dots (QDs) embedded within deformed giant unilamellar vesicles (GUVs). The results show that the distribution of CsPbBr3 QDs differs significantly from that of conventional fluorophores and quasispherical CdSe core/ZnS shell QDs, suggesting that the geometry of both QDs and lipid membranes affect the curvature preferences of QDs. These findings provide insights into the aggregation of proteins induced by curvature and the interactions between lipid membranes and intercalating particles.
Article
Biochemistry & Molecular Biology
Adriano Caliari, Martin M. Hanczyc, Masayuki Imai, Jian Xu, Tetsuya Yomo
Summary: Artificial cells are being used to engineer cell-mimicking structures, with giant unilamellar vesicle fusion being a crucial step. Several techniques have been developed to accomplish this, but automation of fusion detection and characterization is now necessary for high-throughput screenings. We implemented a fusion assay based on fluorophore colocalization to assess lipid and content transfer in vesicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Salah Eddine Ghellab, Xinyuan Zhang, Yicheng Yang, Shuo Wang, Majid Basharat, Xuemao Zhou, Lijie Lei, Ye Zhou, Yufeng Wang, Hui Fang, Yongxiang Gao
Summary: A hybrid system composed of colloidal motors and self-assembled lipid tubes is designed to mimic the subcellular traffic system in living cells, enabling directional delivery of lipid vesicles. The assembly and function of the hybrid system are rationalized by a cooperative action of light-triggered electrophoretic and hydrodynamic effects.
Article
Biochemistry & Molecular Biology
Himadri Gourav Behuria, Bijesh Kumar Biswal, Santosh Kumar Sahu
Summary: A novel method for electroformation of liposomes and phytosomes using copper electrode was described in this study. The liposomes and phytosomes prepared showed various diameters, high stability, and encapsulation efficiency. This easy, inexpensive and convenient method is proven to be useful for preparation of liposomes and phytosomes.
JOURNAL OF LIPOSOME RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Mary H. Wood, David C. Milan, Richard J. Nichols, Michael T. L. Casford, Sarah L. Horswell
Summary: The efficacy of various methods for depositing DMPC lipid bilayers onto a silicon substrate was investigated, with the addition of calcium ions or cholesterol improving coverage and decreasing defects, while vesicle fusion formation proved to be a more reliable but potentially multilayer-forming method.
Article
Food Science & Technology
Feng Jiang, Jingbo Liu, Zhiyang Du, Xuanting Liu, Xiaomin Shang, Yiding Yu, Ting Zhang
Summary: This study investigates the effect of soybean meal peptides on phase separation and reveals the correlation between peptide membrane localization and phase regulation. The results demonstrate that peptides YYK, CLA, and SLW increase the liquid-ordered (Lo) phase while peptide WLQ decreases it. The localization of peptides in the membrane amphiphilic region plays a crucial role in their regulation of phase separation.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Materials Science, Biomaterials
Kirian Tallo, Ramon Pons, Cesar Gonzalez, Olga Lopez
Summary: Colloidal gels made from lipid vesicles under highly diluted conditions have been described, which could be relevant for mimicking biological conditions. Mechanistic insights revealed that freezing and heating are crucial steps in gel formation, and techniques such as transmission electron microscopy, differential scanning calorimetry, and synchrotron small angle X-ray scattering allow for in-depth analysis.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Physical
Adam Juhasz, Ditta Ungor, Katalin Berta, Laszlo Seres, Edit Csapo
Summary: In this study, a spreadsheet routine was demonstrated to provide a universal and reproducible data analysis strategy for in vitro dissolution profiles of encapsulated drugs without the need for linearization. Different liposome-based drug carrier systems were prepared and characterized, showing that the remote loading encapsulation technique increased the half-life of RhoB from 0.8 h to nearly two hours. The importance of the application way of the chosen kinetic model was highlighted, emphasizing the need to consider the appropriate mathematical model beyond just linear forms.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Deepthi Priyanka Damera, Amit Nag
Summary: This study investigates the alterations in membrane fluidity caused by functionalization of polymers and preparation of giant polymersomes. The functionalized vesicles were able to achieve pH-sensitive release of curcumin with tumor-targeting capability, demonstrating their potential in drug delivery applications.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Physical
Arnir H. Bahrami, Michael Raatz, Jaime Agudo-Canalejo, Raphael Michel, Emily M. Curtis, Carol K. Hall, Michael Gradzielski, Reinhard Lipowsky, Thomas R. Weikl
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2014)
Article
Chemistry, Multidisciplinary
Ingo Hoffmann, Raphael Michel, Melissa Sharp, Olaf Holderer, Marie-Sousai Appavou, Frank Polzer, Bela Farago, Michael Gradzielski
Article
Chemistry, Physical
Raphael Michel, Tobias Plostica, Ludmila Abezgauz, Dganit Danino, Michael Gradzielski
Article
Multidisciplinary Sciences
Raphael Michel, Lena Poirier, Quentin van Poelvoorde, Josette Legagneux, Mathieu Manassero, Laurent Corte
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Biochemistry & Molecular Biology
Raphael Michel, Rachel Auzely-Velty
Article
Polymer Science
Fatma Abdi, Raphael Michel, Robin Poirot, Malika Dakir, Lucie Sancey, Valerie Ravaine, Rachel Auzely-Velty
MACROMOLECULAR RAPID COMMUNICATIONS
(2020)
Article
Nanoscience & Nanotechnology
Raphael Michel, Mailie Roquart, Elodie Llusar, Fabrice Gaslain, Sophie Norvez, Jae Seon Baik, Gi-Ra Yi, Mathieu Manassero, Laurent Corte
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Physical
Tamiris Figueiredo, Vanina Cosenza, Yu Ogawa, Isabelle Jeacomine, Alicia Vallet, Sonia Ortega, Raphael Michel, Johan D. M. Olsson, Thibaud Gerfaud, Jean-Guy Boiteau, Jing Jing, Craig Harris, Rachel Auzely-Velty
Article
Chemistry, Physical
Verena Dahl, Achim Friedrich, Juergen Meyer, Joachim Venzmer, Lhoussaine Belkoura, Reinhard Strey, Christian Mayer, Raphael Michel, Michael Gradzielski
COLLOIDS AND INTERFACES
(2018)