期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 12, 期 2, 页码 1146-1160出版社
MDPI AG
DOI: 10.3390/ijms12021146
关键词
Acacia confusa; stem bark; polymeric procyanidins; fractionation; antioxidant activity; MALDI-TOF MS; RP-HPLC
资金
- National Natural Science Foundation of China [31070522]
- Program for New Century Excellent Talents in University [NCET-07-0725]
The polymeric procyanidins extracted from Acacia confusa stem bark were fractionated with a step gradient of water, methanol and acetone on a Sephadex LH-20 column. The antioxidant activity of the collected fractions was investigated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing/antioxidant power (FRAP) assays. All fractions possessed potent antioxidant activity with the highest activity observed for fraction F9. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and reversed-phase high performance liquid chromatography (RP-HPLC) analyses suggested that the collected fractions consisted primarily of oligomeric and polymeric procyanidins, with different polymer ranges and most abundant polymer size. For each fraction, catechin and epicatechin were present as both terminal and extension units, and epicatechin was the major component in the extended chain. The mean degree of polymerization (mDP) of each fraction differed, ranging from 1.68 (fraction F2) to 17.31 (fraction F11). There was a relationship between antioxidant activity (IC50/DPPH and FRAP) and mDP (R-DPPH(2) = 0.861, P = 0.006 and R-FRAP(2) = 0.608, P = 0.038), respectively. However, the highest antioxidant activity of fraction (F9) was not coincident with the maximum mDP of fraction (F11).
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