Article
Chemistry, Applied
Haipeng Su, Li Gao, Jianan Sun, Xiangzhao Mao
Summary: This study successfully modified the ChBDs of chitinase SaChiA4 to improve enzymatic activity, leading to advancements in industrial-scale hydrolysis and utilization of insoluble chitin.
Article
Chemistry, Applied
Daisuke Yamanaka, Kento Suzuki, Masahiro Kimura, Fumitaka Oyama, Yoshiyuki Adachi
Summary: In this study, we developed a reliable method for chitin detection using a luciferase-fragment complementation assay. The use of a CatD mutant allowed for higher sensitivity and structure-specific detection of chitin. Furthermore, a sandwich ELISA using modified CatD showed a low detection limit for soluble chitin.
CARBOHYDRATE POLYMERS
(2022)
Article
Agriculture, Multidisciplinary
Jing Li, Damao Wang, Shu-Chieh Chang, Pi-Hui Liang, Vaibhav Srivastava, Shih-Yun Guu, Jiun-Jie Shie, Kay-Hooi Khoo, Vincent Bulone, Yves S. Y. Hsieh
Summary: Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications, and their biological activities are largely dependent on their structural properties, especially the degree of polymerization (DP) and the position of acetyl groups. A newly discovered chitinase from Paenibacillus pabuli (PpChi) has been shown to produce structurally defined paCOSs with specific compositions, demonstrating stability and activity over a wide temperature range, making it a promising biocatalyst for industrial processes. When used in combination with a recently discovered fungal oxidase, PpChi significantly increases the release of oligosaccharides, contributing to the green production of valuable paCOSs with a well-defined structure.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Chemistry, Medicinal
Xiaojun Zhang, Jianbo Yuan, Fuhua Li, Jianhai Xiang
Summary: Chitin synthesis genes typically occur in single or two copies in crustaceans, while chitin degradation genes are all multiple copies. Chitinase genes are significantly expanded in most crustacean genomes. The gene structure and expression pattern of these genes are similar to those of insects, albeit with some specific characteristics.
Article
Chemistry, Medicinal
Xiyan Wang, Thomas Isbrandt, Mikael Lenz Strube, Sara Skott Paulsen, Maike Wennekers Nielsen, Yannick Buijs, Erwin M. Schoof, Thomas Ostenfeld Larsen, Lone Gram, Sheng-Da Zhang
Summary: Genome mining of pigmented Pseudoalteromonas has shown potential for bioactive compound and enzyme production. Proteomic analysis of Pseudoalteromonas rubra strain S4059 revealed an efficient chitin degradation system when grown on chitin. Deletion of GH19 chitinase in S4059 did not affect chitin degrading ability or secondary metabolite profile, suggesting its industrial potential.
Article
Agricultural Engineering
Manish Kumar, Jogi Madhuprakash, Venkatesh Balan, Amit Kumar Singh, V. Vivekanand, Nidhi Pareek
Summary: This study aimed to develop a two-step chemoenzymatic method for producing short chain chitooligosaccharides. By pretreating chitin with various chemicals and enzymes, and optimizing the conditions, the production of chitooligosaccharides was increased. Furthermore, the purification of the products was achieved using cation exchange chromatography, laying the foundation for sustainable upcycling of coastal residual resources.
BIORESOURCE TECHNOLOGY
(2021)
Article
Entomology
Ming-Bo Qu, Shao-Peng Sun, Yuan-Sheng Liu, Xiao-Rui Deng, Jun Yang, Qing Yang
Summary: The insect group II chitinase plays an important role in insect molting and acts synergistically with other chitinases to degrade cuticular chitin efficiently.
Article
Food Science & Technology
Jiran Lv, Yumeng Zhang, Meihu Ma, Deog-Hwan Oh, Xing Fu
Summary: A novel chitinase (EaChi40) was discovered from a marine bacterium, which efficiently degraded crayfish shell and produced chitin oligosaccharides, showing promising applications in the food and biological fields.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Kazuaki Okawa, Eri Tabata, Yuta Kida, Kyohei Uno, Hidetoshi Suzuki, Minori Kamaya, Peter O. O. Bauer, Fumitaka Oyama
Summary: This study investigates the loss of enzymatic activity in the Ym1 protein compared to Chia. It identifies three protein segments responsible for the loss: the catalytic motif residues, exons 6 and 7, and exon 10. The study also finds extensive gene duplication events specific to rodent lineages at the Ym1 locus and suggests that amino acid substitutions in regions involved in chitin recognition, binding, and degradation lead to irreversible inactivation of the protein.
Article
Biochemistry & Molecular Biology
Lu Li, Yu-Qin Wang, Guang-Ya Li, Qi-Sheng Song, David Stanley, Shu-Jun Wei, Jia-Ying Zhu
Summary: Chitin plays a significant role in insects, and enzymes involved in chitin metabolism are crucial for insect development and metamorphosis. Through bioinformatics analysis, we identified 54 genes encoding putative chitin metabolism enzymes in the genome of the yellow mealworm. These genes showed dynamic expression patterns during molting and were predominantly expressed in specific tissues or body parts, indicating their important roles in molting and development.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Aijia Xing, Yang Hu, Wei Wang, Francesco Secundo, Changhu Xue, Xiangzhao Mao
Summary: A novel chitinase gene from Streptomyces bacillaris was cloned and expressed in Escherichia coli BL21. The purified recombinant enzyme (SbChiAJ103) exhibited exochitinase activity and was immobilized on magnetic nanoparticles (MNPs) using mono-methyl adipate as a linker. The immobilized SbChiAJ103@MNPs showed improved pH tolerance, temperature stability, and storage stability compared to the free enzyme. The immobilization of SbChiAJ103 paves the way for efficient and eco-friendly commercial production of (GlcNAc)2.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qiong Zhao, Liqiang Fan, Chen Deng, Chunyu Ma, Chunyue Zhang, Liming Zhao
Summary: Chitinase ChiC8-1 with high chitin-binding capacity was purified and characterized in this study. A modified affinity chromatography method was developed to purify ChiC8-1 and hydrolyze chitin simultaneously. This process simplifies the purification steps and enables direct production of chitin oligosaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Soil Science
Elias K. Zegeye, Vivian S. Lin, Jamie R. Nunez, Nick A. Sconzo, Samuel O. Purvine, Aaron T. Wright, James J. Moran
Summary: Chitin is a highly abundant soil biopolymer that plays a key role in the carbon and nitrogen cycles. Understanding the factors that control chitin breakdown under different environmental conditions requires further research. Rhizosphere microbiomes and root exudates have been found to be important in chitin catabolism.
SOIL BIOLOGY & BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Yongmei Lyu, Feng Zheng, Chuanxing Qiu, Meng Wang, Dujun Wang, Xiaoyang Zhang, Josef Voglmeir, Li Liu, Xiaohong Yu
Summary: A previously unstudied chitinase gene (MxChi) from Myxococcus xanthus was cloned, expressed, and purified, demonstrating efficient hydrolysis of colloidal chitin to chitobiose as sole product. The optimal catalytic conditions were identified, and in combination with other enzymes GlcN was successfully extracted from chitin sources. The newly discovered chitinase exhibits potential for green recycling of chitin-rich waste due to its facile recombinant in E. coli, robust thermostability, and high purity of the product.
Article
Chemistry, Medicinal
Jianrong Wang, Mujin Zhu, Ping Wang, Wei Chen
Summary: A cold-adapted and efficient chitinase (ChiTg) was purified and characterized from the marine Trichoderma gamsii R1, which showed high activity and stability in cold conditions. It can efficiently degrade colloidal chitin into chitin oligosaccharides with various biological applications. This study provides a cold-active and efficient chitinase for the preparation of chitin oligosaccharides.
Article
Evolutionary Biology
Horng-Yunn Dou, Yih-Yuan Chen, Ying-Tsong Chen, Jia-Ru Chang, Chien-Hsing Lin, Keh-Ming Wu, Ming-Shian Lin, Ih-Jen Su, Shih-Feng Tsai
EVOLUTIONARY BIOINFORMATICS
(2016)
Article
Multidisciplinary Sciences
Horng-Yunn Dou, Chien-Hsing Lin, Yih-Yuan Chen, Shiu-Ju Yang, Jia-Ru Chang, Keh-Ming Wu, Ying-Tsong Chen, Pei-Ju Chin, Yen-Ming Liu, Ih-Jen Su, Shih-Feng Tsai
SCIENTIFIC REPORTS
(2017)
Article
Immunology
Min-Chi Lu, Ying-Tsong Chen, Ming-Ko Chiang, Yao-Chen Wang, Pei-Yi Hsiao, Yi-Jhen Huang, Ching-Ting Lin, Ching-Chang Cheng, Chih-Lung Liang, Yi-Chyi Lai
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Ying-Tsong Chen, Yi-Chyi Lai, Mei-Chen Tan, Li-Yun Hsieh, Jann-Tay Wang, Yih-Ru Shiau, Hui-Ying Wang, Ann-Chi Lin, Jui-Fen Lai, I-Wen Huang, Tsai-Ling Lauderdale
SCIENTIFIC REPORTS
(2017)
Letter
Microbiology
Yao-Chen Wang, Hui-Ling Tang, Yu-Chieh Liao, Chien-Shun Chiou, Ying-Tsong Chen, Ming-Ko Chiang, Min-Chi Lu, Yi-Chyi Lai
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Infectious Diseases
Min-Chi Lu, Ying-Tsong Chen, Hui-Ling Tang, Yen-Yi Liu, Bo-Han Chen, You-Wun Wang, Yi-Syong Chen, Ru-Hsiou Teng, Yu-Ping Hong, Chien-Shun Chiou, Ming-Ko Chiang, Yi-Chyi Lai
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Correction
Microbiology
Yao-Chen Wang, Hui-Ling Tang, Yu-Chieh Liao, Chien-Shun Chiou, Ying-Tsong Chen, Ming-Ko Chiang, Min-Chi Lu, Yi-Chyi Lai
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Microbiology
Yu-Ping Hong, Ying-Tsong Chen, You-Wun Wang, Bo-Han Chen, Ru-Hsiou Teng, Yi-Syong Chen, Chien-Shun Chiou
Summary: In cases of human salmonellosis in Taiwan between 2014 and 2019, a study found that 26.4% of multidrug-resistant Salmonella enterica serovar Albany isolates carried an erm42-carrying integrative and conjugative element, ICE_erm42. Strains carrying ICE_erm42 exhibited high-level resistance to azithromycin and could transfer into phylogenetically distant specie Vibrio cholerae via conjugation.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Public, Environmental & Occupational Health
Y-L Lee, K-M Liu, H-L Chang, J-S Lin, F-Y Kung, C-M Ho, K-H Lin, Y-T Chen
Summary: An outbreak of Elizabethkingia anophelis bacteraemia occurred in a respiratory care center of a tertiary hospital in Taiwan from 2015 to 2018. The outbreak strain was identified to be specific and distinct from other known strains worldwide by complete-genome sequencing analysis. Contamination of hospital tap water and inanimate surfaces played a role in the transmission, leading to the implementation of specific infection control strategies to control the outbreak.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Multidisciplinary Sciences
Yu-Shan Huang, Wan-Chen Tsai, Jia-Jie Li, Pao-Yu Chen, Jann-Tay Wang, Ying-Tsong Chen, Feng-Jui Chen, Tsai-Ling Lauderdale, Shan-Chwen Chang
Summary: New Delhi metallo-beta-lactamase (NDM) is the most prevalent carbapenemase among carbapenemase-producing Escherichia coli (CPEc) in Taiwan, with IncX3 plasmid and globally distributed strain ST410 potentially contributing to its rapid dissemination.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Yu-Ping Hong, You-Wun Wang, Bo-Han Chen, Hui-Yung Song, Chien-Shun Chiou, Ying-Tsong Chen
Summary: The investigation into the epidemiological trends of Salmonella enterica serovar Goldcoast revealed closely related strains with varying drug resistances. The comparison of two multidrug-resistant strains led to the identification of an additional ramA gene that can be transmitted through plasmids among bacteria. Introducing this gene into Escherichia coli resulted in elevated expression of efflux pump genes and increased minimum inhibitory concentrations to multiple antibiotics.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Cell Biology
Yun-Hsin Cheng, Ying-Chieh Ko, Hsiang-Ju Ku, Ching-Chun Huang, Yu-Ching Yao, Yi-Tzu Liao, Ying-Tsong Chen, Shiu-Feng Huang, Li-Rung Huang
Summary: The paper describes two HCC cell lines with distinct features of lipid metabolism related to cancer stemness and differential interplay with the immune system. The authors present this syngeneic HCC mouse model as a practical tool for studying cancer stemness and discovering new therapies targeting liver cancers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Yu-Lin Lee, Kuan-Ming Liu, Hui-Lan Chang, Yi-Ci Liao, Jen-Shiou Lin, Fang-Yen Kung, Cheng-Mao Ho, Kai-Hsiang Lin, Ying-Tsong Chen
Summary: The incidence of Elizabethkingia anophelis bacteremia has increased significantly in Changhua Christian Hospital since 2013. A specific lineage of E. anophelis strains with unique genomic features has emerged and shown resistance to antibiotics. This finding has important implications for further research and developing effective treatment strategies.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Kuan-Ming Liu, Hui-Lan Chang, Ming-Hong Hsu, Yi-Zhen Lin, Yu-Lin Lee, Ying-Tsong Chen
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Microbiology
Chien-Shun Chiou, Ying-Tsong Chen, You-Wun Wang, Yen-Yi Liu, Hung-Chih Kuo, Yeuh-Hua Tu, Ann-Chi Lin, Ying-Shu Liao, Yu-Ping Hong
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)