Article
Biochemistry & Molecular Biology
Gustavo Carvalho, Bruno Marcal Repoles, Isabela Mendes, Paulina H. Wanrooij
Summary: Research has focused on maintenance and instability of mitochondrial DNA, exploring the role of DNA repair factors in mitochondria and the connection between mtDNA damage and immune response. Future studies should delve into mechanisms by which mitochondria maintain mtDNA, the balance between repair and degradation, as well as the release of mtDNA into the cytosol.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Multidisciplinary Sciences
Pierre R. Bushel, James Ward, Adam Burkholder, Jianying Li, Benedict Anchang
Summary: The interplay between genetic variants in the nuclear and mitochondrial genomes plays a crucial role in breast cancer development, affecting the size of cell nuclei and the risk of mortality.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Anna Haupts, Anne Vogel, Sebastian Foersch, Monika Hartmann, Annett Maderer, Nicolas Wachter, Tobias Huber, Werner Kneist, Wilfried Roth, Hauke Lang, Markus Moehler, Nils Hartmann
Summary: The study found that liquid biopsies using cfDNA from blood plasma are an effective means of diagnosing colorectal cancer and complementary to tissue biopsies. Analysis of cfDNA level and fragment size during chemotherapy showed that changes in cfDNA content and fragment size can reflect treatment response, while alterations in cfDNA characteristics may indicate tumor recurrence.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Orsolya Pipek, Donat Alpar, Orsolya Rusz, Csaba Bodor, Zoltan Udvarnoki, Anna Medgyes-Horvath, Istvan Csabai, Zoltan Szallasi, Lilla Madaras, Zsuzsanna Kahan, Gabor Cserni, Bence Kovari, Janina Kulka, Anna Maria Tokes
Summary: This study conducted whole-genome sequencing on 63 samples from 29 Hungarian breast cancer patients, revealing common pathogenic genes and mutational signatures related to homologous recombination deficiency. This highlights the importance of comprehensive genomic characterization in breast cancer patient populations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Xiaoying Ji, Wenjie Guo, Xiwen Gu, Shanshan Guo, Kaixiang Zhou, Liping Su, Qing Yuan, Yang Liu, Xu Guo, Qichao Huang, Jinliang Xing
Summary: The study reveals a significantly higher and more variable mutation rate in mtCTR across different tumor types, with a noticeable distributional bias of tumor somatic mutations between the hypervariable segment and non-hypervariable segment. The mutational pattern of mtCTR is associated with mitochondrial biogenesis, mitochondrial oxidative metabolism, and patient overall survival.
Article
Biochemical Research Methods
Xiangwen Ji, Edwin Wang, Qinghua Cui
Summary: Somatic mutational signatures identified by genome sequencing are important in understanding the cause and development of cancer. The computational framework GSGP can decipher the molecular mechanisms of these signatures and has extended their applications to RNA sequencing. Using GSGP, new signatures have been identified and the etiologies for certain signatures have been revealed, such as an RNA-derived signature involved in colorectal cancer.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Genetics & Heredity
Xiaole Zhu, Rong Yu, Yunpeng Peng, Yi Miao, Kuirong Jiang, Qiang Li
Summary: In this study, a genomic instability-related lncRNA signature (GILncSig) was identified in pancreatic cancer (PC) patients, and a prognostic model was developed based on this signature. The GILncSig showed better predictive ability than existing lncRNA-related signatures in terms of survival, and may serve as novel prognostic biomarkers with potential implications in immunotherapy decisions for PC patients.
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Elizabeth Chun Yong Lee, Jessica Sook Ting Kok, Bin Tean Teh, Kah Suan Lim
Summary: Genome instability and immune evasion are crucial characteristics of cancer, and targeting them can effectively treat the disease. Both genotoxic therapy and immunotherapy have shown synergistic effects in treating cancer, with different mechanisms at play.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Chunyu Liu, Jessica L. Fetterman, Yong Qian, Xianbang Sun, Thomas W. Blackwell, Achilleas Pitsillides, Brian E. Cade, Heming Wang, Laura M. Raffield, Leslie A. Lange, Pramod Anugu, Goncalo Abecasis, L. Adrienne Cupples, Susan Redline, Adolfo Correa, Ramachandran S. Vasan, James G. Wilson, Jun Ding, Daniel Levy
Summary: The study found significant differences in mitochondrial DNA heteroplasmic mutations in maternal pairs in terms of concordance and discordance, with the number of deleterious heteroplasmies increasing significantly with age. Furthermore, an increase in heteroplasmic burden was strongly associated with a decrease in average number of mtDNA copy number in blood.
Review
Endocrinology & Metabolism
Agnieszka H. Ludwig-Slomczynska, Markus Rehm
Summary: Cellular energy metabolism is regulated by two genomes, the nuclear genome and the mitochondrial genome, which coevolve and affect cellular processes and metabolic diseases. Variations in the mitochondrial genome can influence cellular metabolism and physiology, and are associated with the onset of metabolic diseases.
Article
Cell Biology
Maoni Guo, San Ming Wang
Summary: A computational approach was developed to identify a prognostic signature of 11 genes in TNBC, which successfully classified TNBC cases into high- and low-risk groups and showed predictive power in multiple datasets. This novel signature provides a better understanding of genome instability in TNBC and can serve as prognostic markers for clinical TNBC management.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Satu Maki-Nevala, Sanjeevi Ukwattage, Alisa Olkinuora, Henrikki Almusa, Maarit Ahtiainen, Ari Ristimaki, Toni Seppala, Anna Lepisto, Jukka-Pekka Mecklin, Paivi Peltomaki
Summary: Ulcerative colitis increases the risk of colorectal cancer, and this study examined the genetic and epigenetic profiles of colitis-associated colorectal carcinomas (CA-CRC). Subgroups within CA-CRC were identified with different mutational loads and signatures, which may guide clinical management and therapy options. Lynch syndrome tumors showed a higher degree of hypermutability compared to CA-CRC subgroups.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Cell Biology
Tung Chao, Hsueh-Tzu Shih, Shih-Chin Hsu, Pei-Jer Chen, Yu-Shan Fan, Yung-Ming Jeng, Zhao-Qing Shen, Ting-Fen Tsai, Zee-Fen Chang
Summary: Genotoxic insult leads to damages in nuclear and mitochondrial DNA with autophagy induction. The study reveals that specific nuclear DNA damage alone does not require autophagy for repair, unless there is mitochondrial DNA damage present. Furthermore, autophagy suppression causes increased mitochondrial permeability, releasing mitochondrial ENDOG to the nuclei and sustaining nuclear DNA damage.
Review
Genetics & Heredity
Lin Fu, Yu-Xin Luo, Ying Liu, Hui Liu, Hong-zhen Li, Yang Yu
Summary: Research has shown that changes and functions of mitochondrial DNA play a crucial role in gamete quality and fertility during gametogenesis and fertilization. Breakthroughs in mitochondrial genome editing offer great potential for the treatment of diseases related to mtDNA mutations.
FRONTIERS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Burair A. Alsaihati, Kun-Lin Ho, Joshua Watson, Yuan Feng, Tianfang Wang, Kevin K. Dobbin, Shaying Zhao
Summary: Canine tumor alteration landscape is tumor type-dependent, but likely breed-independent. Major pathway alterations are found in each tumor type, with varying tumor mutational burden.
NATURE COMMUNICATIONS
(2021)
Article
Clinical Neurology
Shih-Pin Chen, Yi-An Chang, Chih-Hung Chou, Chi-Chang Juan, Hsin-Chen Lee, Luen-Kui Chen, Pei-Chun Wu, Yen-Feng Wang, Jong-Ling Fuh, Jiing-Feng Lirng, Anne Ducros, Hsien-Da Huang, Shuu-Jiun Wang
Summary: This study identified a panel of miRNAs that distinguished patients with RCVS from controls, with let-7a-5p, let-7b-5p, and let-7f-5p showing higher abundance in patients with ictal migraine. Target prediction and pathway analysis suggested their involvement in RCVS pathogenesis, which was confirmed in vitro. MiR-130a-3p was associated with disruption of the blood-brain barrier and increased permeability in a human BBB model.
ANNALS OF NEUROLOGY
(2021)
Article
Oncology
Chun-Yu Liu, Tzu-Ting Huang, Ji-Lin Chen, Pei-Yi Chu, Chia-Han Lee, Hsin-Chen Lee, Yu-Hsuan Lee, Yuan-Ya Chang, Shung-Haur Yang, Jeng-Kai Jiang, Wei-Shone Chen, Yee Chao, Hao-Wei Teng
Summary: High expression of KMO in CRC is associated with higher metastasis and poorer survival rates. Knockdown of KMO reduces the expression of cancer stem cell markers and the invasion abilities of CRC cells, while inhibition of KMO enzymatic activity suppresses CRC cell motility.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mei-Chun Chen, Li-Lin Hsu, Sheng-Fan Wang, Yi-Ling Pan, Jeng-Fan Lo, Tien-Shun Yeh, Ling-Ming Tseng, Hsin-Chen Lee
Summary: This study revealed that activation of the integrated stress response (ISR) through salubrinal under glucose deprivation conditions increased cancer cell death by upregulating xCT and inducing mitochondrial oxidative stress.
Article
Oncology
Sheng-Fan Wang, Yuh-Lih Chang, Yen-Dun Tzeng, Chun-Ling Wu, Yuan-Zhong Wang, Ling-Ming Tseng, Shiuan Chen, Hsin-Chen Lee
Summary: The study highlights the association between mitochondrial alterations and AI resistance in breast cancer patients, indicating that changes in mitochondrial function could impact patient prognosis. Induction of drug resistance in breast cancer cells through mitochondrial stress adaptation, possibly due to the activation of the AREG-ER alpha loop signaling, suggest potential treatment targets for overcoming AI resistance.
Article
Biochemistry & Molecular Biology
Tzu-Ching Chang, Hui-Ting Lee, Siao-Cian Pan, Shih-Han Cho, Chieh Cheng, Liang-Hung Ou, Chia- Lin, Chen-Sung Lin, Yau-Huei Wei
Summary: We investigated the roles of mitochondrial DNA (mtDNA) alterations and mitochondrial respiratory dysfunction in gastric adenocarcinoma (GAC) using gastric cancer cell line AGS and clinical samples. The study found that decreased mtDNA copy number and D310 mutation were associated with the development and prognosis of GAC, and mitochondrial dysfunction affected the biological characteristics of GAC cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Chih-Hao Wang, Chen-Hung Wang, Pen-Jung Hung, Yau-Huei Wei
Summary: The prevalence and healthcare burden of obesity and its related metabolic disorders such as type 2 diabetes (T2D) are increasing rapidly. Mitochondria and endoplasmic reticulum (ER) play important roles in insulin resistance and T2D, and their dysfunction may contribute to the progression of these diseases. In this study, the authors found that the formation of mitochondria-associated ER membranes (MAMs) is increased during the differentiation of preadipocytes, and disruption of MAMs impairs cell differentiation and insulin signaling. Additionally, knocking down the protein GRP75, which is involved in MAMs formation, also impairs thermogenesis and glucose utilization in brown adipocytes. These findings suggest that MAMs formation is essential for the proper functioning of adipocytes and insulin signaling, and may be a potential target for therapeutic interventions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Sheng-Fan Wang, Yuh-Lih Chang, Wen-Liang Fang, Anna Fen-Yau Li, Chian-Feng Chen, Tien-Shun Yeh, Giun-Yi Hung, Kuo-Hung Huang, Hsin-Chen Lee
Summary: This study investigates the role of growth differentiation factor 15 (GDF15) in chemoresistance of gastric cancer. The results demonstrate that GDF15 serum levels can serve as a valuable biomarker for predicting prognosis in gastric cancer. Additionally, GDF15 upregulation is associated with cisplatin resistance in gastric cancer cells by increasing intracellular glutathione (GSH) content and antioxidant activities.
Review
Endocrinology & Metabolism
Sheng-Fan Wang, Yi-Sheng Lin, Wan-Yu Yeh, Yuh-Lih Chang, Chern-En Chiang, Chen-Huan Chen, Ling-Ming Tseng, Hsin-Chen Lee, Chun-Yu Liu, Hao-Min Cheng
Summary: This study summarizes the clinical effects of antiresorptive agents in patients with early breast cancer receiving endocrine therapy. The results show that antiresorptive agents have significant clinical benefits on disease recurrence and locoregional recurrence in these patients. However, there is a potential risk of osteonecrosis of the jaw with the use of these agents.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Oncology
Sheng-Fan Wang, Kuan-Wei Huang, Yueh-Ching Chou, Hsin-Chen Lee, Po-Kuei Wu, Wei-Ming Chen, Giun-Yi Hung, Yuh-Lih Chang
Summary: Taiwanese patients receiving high-dose methotrexate (HD-MTX) treatment often experience severe hepatotoxicity, which affects subsequent treatment. Co-administration of proton pump inhibitors (PPIs), trimethoprim-sulfamethoxazole (TMP-SMX), or non-steroidal anti-inflammatory drugs (NSAIDs) with MTX can lead to drug-drug interactions. However, limited real-world analyses have been conducted on the effects of these co-medications and other potential risk factors on HD-MTX-mediated acute hepatotoxicity in patients with osteogenic sarcoma (OGS) in East Asia.
Article
Cell Biology
Yu-Ting Wu, Hui-Yi Tay, Jung-Tse Yang, Hsiao-Hui Liao, Yi-Shing Ma, Yau-Huei Wei
Summary: In this study, patient-derived iPSCs were used to model neurons and investigate the pathogenic mechanism of neurological disorders in MERRF syndrome. The results showed that MERRF neural cells harboring the m.8344A > G mutation exhibited impaired mitochondrial bioenergetic function, elevated ROS levels and imbalanced expression of antioxidant enzymes. Electrophysiological recordings also revealed impairment of neuronal activities in neurons with a high level of the m.8344A > G mutation.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Review
Cell Biology
Sheng-Fan Wang, Ling-Ming Tseng, Hsin-Chen Lee
Summary: Dysregulating cellular metabolism is an emerging cancer hallmark, and mitochondria play a crucial role in cancer progression. This review discusses the alterations in mitochondrial DNA and dynamics in cancer, as well as the regulatory mechanisms of mitochondrial dysfunction-mediated retrograde signaling pathways. It also explores the impact of mitochondria on immune regulation and the potential of targeting mitochondria and metabolism for cancer immunotherapy.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sheng-Fan Wang, Yuh-Lih Chang, Ting-Yu Liu, Kuo-Hung Huang, Wen-Liang Fang, Anna Fen-Yau Li, Tien-Shun Yeh, Giun-Yi Hung, Hsin-Chen Lee
Summary: This study found that high expression of GDF15 protein may increase disease recurrence in adjuvant chemotherapy-treated gastric cancer patients. Additionally, treatment with mitochondrial inhibitors can upregulate GDF15 and enhance cisplatin insensitivity in human gastric cancer cells. Mechanistically, the activating transcription factor 4-C/EBP homologous protein pathway plays a crucial role in the heightened manifestation of GDF15. This research reveals that mitochondrial dysfunction enhances cisplatin insensitivity through GDF15 upregulation.
Article
Allergy
Vincent Yi-Fong Su, Szu-Wen Ko, Yuh-Lih Chang, Yueh-Ching Chou, Hsin-Chen Lee, Kuang-Yao Yang, Kun-Ta Chou, Chia-Chen Hsu
Summary: This study found that cardioselective beta-blockers, ARBs, and DHP CCB therapies may lower the risk of acute exacerbation in patients with ACO.
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jagdeep K. Sandhu, Caroline Sodja, Maria Ribecco-Lutkiewicz, Yu-Ting Wu, Yi-Shing Ma, Yau-Huei Wei, Marianna Sikorska
Summary: Mitochondrial biogenesis occurs during neuronal differentiation, leading to improved cellular energy status and enhanced tolerance to oxidative stress, casting doubt on the potential for additional enhancement of this phenomenon for neuroprotection.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Review
Urology & Nephrology
Tzu-Yu Chuang, Te-Wei Chang, Shiou-Sheng Chen, Chan-Chi Chang, Wei-Ming Cheng, Yau-Huei Wei
Summary: Mitochondria are essential organelles responsible for energy production and regulating various cellular processes. The prevalence of primary mitochondrial disease is estimated to be around 1:5000, with implications in bladder dysfunction, reproductive disorders, and bladder cancer due to mtDNA mutations.
UROLOGICAL SCIENCE
(2021)