期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 27, 期 2, 页码 221-226出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2010.570
关键词
stromal cell-derived factor-1; endothelial progenitor cells; menstrual cycle; endometrium
资金
- Swedish Medical Research Council [71X-05991, 71BI-14589]
- Karolinska Institutet, Karolinska University Hospital Huddinge
- Cancer Society of Stockholm
- Swedish Labor Market Insurance
The endometrium goes through a unique cycle of physiological angiogenesis during the normal menstrual cycle (MC). We studied whether there is a correlation between endothelial progenitor cells (EPCs) and plasma and endometrial levels of angiogenic growth factors during the MC. Ten healthy, regularly menstruating women provided blood samples and another 16 supplied endometrial biopsies. Blood samples were obtained over a single MC: twice in the proliferative and once in the secretory phase and at ovulation. Endometrial biopsies were provided in the proliferative or in the secretory phase. We assessed plasma levels of vascular endothelial and fibroblast growth factors, granulocyte and granulocyte-macrophage colony-stimulating factors and stromal cell-derived factor-1 (SDF-1) by ELISA; EPCs by a colony-forming unit (CFU) assay; immunostaining for endometrial SDF-1 by computer-assisted software; and endothelial cell (EC) markers by flow cytometry. In the proliferative phase, SDF-1 levels were significantly higher than during the secretory phase. EPC-CFUs correlated negatively to SDF-1 levels. Endometrial SDF-1 expression tended to be higher in the secretory than in the proliferative phase. Furthermore, vascular endothelial growth factor receptors and Tie-2 EPCs showed a cyclic pattern over the MC. Our results point to SDF-1 as a novel mediator of EPC trafficking during the MC.
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