期刊
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
卷 11, 期 10, 页码 979-987出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijms.9139
关键词
Chronic wounds; mesenchymal stem cells; Wharton's jelly; PVA hydrogel; histology
资金
- FCT, the Portuguese Foundation for Science and Technology [SFRH/BD/76237/2011]
- FCT [ENMED/0002/2010]
- QREN I&DT Cluster in Development of Products for Regenerative Medicine and Cell Therapies - Projects Biomat Cell QREN [2008/1372]
- European Community FEDER fund through ON2 - O Novo Norte - North Portugal Regional Operational Program
- Ministerio da Educacao e da Ciencia
- Program Project Euronanomed [EraNet - EuroNanoMed JTC2010]
- program COMPETE - Programa Operacional Factores de Competitividade [Pest-OE/AGR/UI0211/2011]
The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/mu l hMSCs in a total volume of 100 mu l per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans.
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