期刊
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
卷 302, 期 7-8, 页码 304-314出版社
ELSEVIER GMBH
DOI: 10.1016/j.ijmm.2012.09.004
关键词
Flagella; Adhesion; Human mucus; Probiotic; Escherichia coli Nissle 1917
资金
- Ardeypharm GmbH
The flagellum of the probiotic Escherichia coli strain Nissle 1917 (EcN) is not just responsible for motility, but also for EcN's ability to induce the production of human beta-defensin 2. Here, we report a third function of this EcN organell. In this study we investigated the role of the EcN flagellum in adhesion to different host tissues by ex vivo and in vitro studies. Ex vivo studies with cryosections of human gilt biopsies revealed that the flagellum of EcN is most likely important for efficient adhesion to the human intestinal tract. These results and in vitro studies with different epithelial cells indicated that the presence of mucus is important for efficient mediation of adhesion by the flagellum of EcN. We observed direct interaction between isolated flagella from EcN wild type and porcine mucin 2 as well as human mucus. However, we could not observe any interaction of the flagella with murine mucus. For the first time, we identified the mucus component gluconate as one receptor for the binding of flagella from EcN and were able to exclude the flagellin domain D3 as a responsible interaction partner. We propose that the flagellum of EcN is its major adhesin in vivo, which enables this probiotic strain to compete efficiently for binding sites on host tissue with several bacterial pathogens. (C) 2012 Elsevier GmbH. All rights reserved.
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