期刊
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
卷 301, 期 2, 页码 176-179出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.ijmm.2010.07.004
关键词
Haemophilus influenzae; Bacterial capsule; Serotype; Genotype; DNA primer
资金
- Wellcome Trust
- Robert Koch-Institute, Berlin
A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was >99% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/11164 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences. (C) 2010 Elsevier GmbH. All rights reserved.
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