Article
Microbiology
Yuehui Tian, Tao Peng, Zhenzhen He, Luting Wang, Xurui Zhang, Zhili He, Longfei Shu
Summary: This study uses a novel amoeba-symbiont system to show that the establishment of social amoeba-bacterium symbiosis is not linked with extracellular discrimination, but instead, symbiont-induced phagosome biogenesis contributes to the formation of this symbiotic relationship.
MICROBIOLOGY SPECTRUM
(2022)
Article
Physics, Fluids & Plasmas
Vaibhav Wasnik
Summary: This work presents two results. Firstly, the error in concentration measurement by cells is found to be the same as previously evaluated, even when considering a more realistic case. Secondly, a lower bound on the measurement time is derived, which is independent of diffusion rate and concentration of the chemoattracts, and is determined by the detachment rate of ligands from cell receptors. This finding can help explain experimental observations.
Article
Biochemistry & Molecular Biology
Simone Vormittag, Dario Huesler, Ina Haneburger, Tobias Kroniger, Aby Anand, Manuel Prantl, Caroline Barisch, Sandra Maass, Doerte Becher, Francois Letourneur, Hubert Hilbi
Summary: Legionella pneumophila replicates within a unique compartment called the Legionella-containing vacuole (LCV), which is formed in macrophages and amoeba. The formation of LCV involves lipid conversion and fusion with ER-derived vesicles. Proteomics analysis shows the presence of membrane contact sites (MCS) proteins involved in lipid exchange. Additionally, various proteins such as VAMP-associated protein (Vap), Sac1, and oxysterol binding proteins (OSBPs) play important roles in LCV remodeling and bacterial replication.
Article
Multidisciplinary Sciences
Richa Karmakar, Timothy Tyree, Richard H. Gomer, Wouter-Jan Rappel
Summary: Chemotaxis plays a crucial role in biological processes where cells are guided by chemical gradients. In Dictyostelium discoideum, cell aggregates are formed through periodic pulses of cAMP and secretion of PDE, allowing for cell motion and dispersion. The competition between cAMP and PDE results in the formation of a gradient directed away from the aggregate, leading to outward cell motion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Sarah Koerber, Jan Faix
Summary: Ena/VASP proteins play a crucial role in macroendocytosis, and their deficiency results in defects in macropinocytosis and phagocytosis. Furthermore, VASP initiates actin assembly to propel phagosomes deeper into the cell after cup closure, resembling intracellular movement of rocketing Listeria cells.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Dario Husler, Bernhard Steiner, Amanda Welin, Bianca Striednig, A. Leoni Swart, Virginie Molle, Hubert Hilbi, Francois Letourneur
Summary: The study reveals that D. discoideum Sey1 plays a crucial role in cell structure, proteolysis, cell motility, and intracellular bacterial replication, particularly in the context of Legionella pneumophila infection.
CELLULAR MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yonas Tekle, Janae M. Lyttle, Maya G. Blasingame, Fang Wang
Summary: Amoebozoa, predominantly consisting of amoeboid unicellular protists, plays an important ecological role in controlling environmental bacteria. This research revealed a wide diversity of bacteria and human pathogens associated with Amoebozoa, including potential endosymbionts and multi-drug resistant pathogens. The study highlights the potential public health concern of Amoebozoa harboring dangerous human pathogens.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Jiaqi Fu, Mowei Zhou, Marina A. Gritsenko, Ernesto S. Nakayasu, Lei Song, Zhao-Qing Luo
Summary: The intracellular pathogen Legionella pneumophila delivers effectors into host cells to modulate host energy metabolism, contributing to its virulence mechanism.
Article
Engineering, Environmental
Maura J. Donohue, Maily Pham, Stephanie Brown, Kaveri M. Easwaran, Stephen Vesper, Jatin H. Mistry
Summary: This study investigated the detection methods of Legionella pneumophila in tap water samples in buildings across the United States and their performance under different water quality conditions. The results showed variations in method performance when using three culture methods and one molecular method. Water quality influenced the detection results of L. pneumophila, especially the total organic carbon and heterotrophic bacterial counts were positively correlated with its detection frequency. Therefore, water quality should be considered when selecting L. pneumophila detection methods.
Article
Cell Biology
Romain Bodinier, Ayman Sabra, Jade Leiba, Anna Marchetti, Otmane Lamrabet, Imen Ayadi, Vedrana Filic, Takefumi Kawata, Igor Weber, Pierre Cosson
Summary: The genetic inactivation of lrrkA leads to enhanced phagocytosis and cell motility in D. discoideum cells, while also causing an abnormal response to folate. Folate increases motility in parental cells but not in lrrkA KO cells, indicating the pivotal role of LrrkA in cellular response to folate. The study identifies gene products involved in the control of intracellular killing, cell motility, and gene transcription, shedding light on how D. discoideum can efficiently migrate, engulf, and kill bacteria when encountering bacterially-secreted folate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Infectious Diseases
Jamie Gorzynski, Bryan Wee, Melissa Llano, Joana Alves, Ross Cameron, Jim McMenamin, Andrew Smith, Diane Lindsay, J. Ross Fitzgerald
Summary: Through whole-genome sequencing, this study investigates the evolutionary and epidemiological relationships of Legionella pneumophila isolates in Scotland over 36 years. The analysis reveals the presence of endemic clones in hospital, community, and travel-associated environments, and suggests the importance of redefining cluster and outbreak definitions. The study highlights the significance of whole-genome sequencing in real-time identification and mitigation of clinically important endemic clones.
Article
Microbiology
Christopher T. D. Price, Hannah E. Hanford, Aruna Vashishta, Mateja Ozanic, Marina Santic, Silvia Uriarte, Yousef Abu Kwaik
Summary: The Dot/Icm type IV secretion system of Legionella pneumophila is crucial for lysosomal evasion and macrophage permissiveness, but polymorphonuclear cells (PMNs) respond to this system by rapidly restricting the pathogen. This response is triggered by the injected amylase effector, LamA, which catalyzes glycogen degradation in PMNs and leads to immunometabolic reprogramming involving increased aerobic glycolysis. Activated PMNs utilize major microbicidal processes, including granule fusion and reactive oxygen species production, resulting in rapid killing of Legionella pneumophila.
Article
Microbiology
Md Tahsin Khan, Araf Mahmud, Mahmudul Hasan, Kazi Faizul Azim, Musammat Kulsuma Begum, Mohimenul Haque Rolin, Arzuba Akter, Shakhinur Islam Mondal
Summary: In this study, a subtractive genomics approach was used to identify potential drug targets and vaccine candidates against Legionella pneumophila. Three promising drug targets and one potential vaccine candidate were identified. The findings contribute to the development of novel therapeutics and vaccine candidates for the treatment and prevention of L. pneumophila infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Sui Lay, Xavier Pearce, Oana Sanislav, Paul Robert Fisher, Sarah Jane Annesley
Summary: Mitochondrial Complex II is composed of four core subunits and mutations in any of these subunits can result in lowered Complex II activity. However, mutations in different subunits are associated with distinct patterns of clinical disease. In this study, knockdown of individual Complex II subunits in Dictyostelium discoideum revealed specific phenotypic defects and mitochondrial dysfunction associated with SdhA knockdown. These defects were mediated by the chronic activation of AMP-activated protein kinase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daisuke Ohtsuka, Nobutoshi Ota, Satoshi Amaya, Satomi Matsuoka, Yo Tanaka, Masahiro Ueda
Summary: The study found that chemotaxis was strongest at a cAMP concentration of 100 nM, with about 16% of cells still exhibiting chemotaxis at a concentration of 10 pM. Additionally, chemotaxis was suppressed at a concentration of 100 pM cAMP due to the self-production and secretion of intracellular cAMP induced by extracellular cAMP.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Tomoaki Nishiyama, Hidetoshi Sakayama, Jan de Vries, Henrik Buschmann, Denis Saint-Marcoux, Kristian K. Ullrich, Fabian B. Haas, Lisa Vanderstraeten, Dirk Becker, Daniel Lang, Stanislav Vosolsobe, Stephane Rombauts, Per K. I. Wilhelmsson, Philipp Janitza, Ramona Kern, Alexander Heyl, Florian Ruempler, Luz Irina A. Calderon Villalobos, John M. Clay, Roman Skokan, Atsushi Toyoda, Yutaka Suzuki, Hiroshi Kagoshima, Elio Schijlen, Navindra Tajeshwar, Bruno Catarino, Alexander J. Hetherington, Assia Saltykova, Clemence Bonnot, Holger Breuninger, Aikaterini Symeonidi, Guru V. Radhakrishnan, Filip Van Nieuwerburgh, Dieter Deforce, Caren Chang, Kenneth G. Karol, Rainer Hedrich, Peter Ulvskov, Gernot Gloeckner, Charles F. Delwiche, Jan Petrasek, Yves Van de Peer, Jiri Friml, Mary Beilby, Liam Dolan, Yuji Kohara, Sumio Sugano, Asao Fujiyama, Pierre-Marc Delaux, Marcel Quint, Gunter Theissen, Martin Hagemann, Jesper Harholt, Christophe Dunand, Sabine Zachgo, Jane Langdale, Florian Maumus, Dominique Van Der Straeten, Sven B. Gould, Stefan A. Rensing
Article
Evolutionary Biology
Falk Hillmann, Gillian Forbes, Silvia Novohradska, Iuliia Ferling, Konstantin Riege, Marco Groth, Martin Westermann, Manja Marz, Thomas Spaller, Thomas Winckler, Pauline Schaap, Gernot Gloeckner
GENOME BIOLOGY AND EVOLUTION
(2018)
Article
Cell Biology
Carmen Mross, Marija Marko, Martina Munck, Gernot Gloeckner, Susanne Motameny, Janine Altmueller, Angelika A. Noegel, Ludwig Eichinger, Vivek S. Peche, Sascha Neumann
Article
Multidisciplinary Sciences
Uwe John, Yameng Lu, Sylke Wohlrab, Marco Groth, Jan Janouskovec, Gurjeet S. Kohli, Felix C. Mark, Ulf Bickmeyer, Sarah Farhat, Marius Felder, Stephan Frickenhaus, Laure Guillou, Patrick J. Keeling, Ahmed Moustafa, Betina M. Porcel, Klaus Valentin, Gernot Gloeckner
Article
Plant Sciences
Huiru Li, Catia Monteiro, Sandra Heinrich, Inka Bartsch, Klaus Valentin, Lars Harms, Gernot Gloeckner, Erwan Corre, Kai Bischof
PHYSIOLOGIA PLANTARUM
(2020)
Article
Ecology
Shivani Rana, Klaus Valentin, Inka Bartsch, Gernot Gloeckner
ECOLOGY AND EVOLUTION
(2019)
Article
Plant Sciences
Catia Marina Machado Monteiro, Huiru Li, Kai Bischof, Inka Bartsch, Klaus Ulrich Valentin, Erwan Corre, Jonas Collen, Lars Harms, Gernot Gloeckner, Sandra Heinrich
Article
Microbiology
Miriam Leonard, Anika Kuehn, Rebekka Harting, Isabel Maurus, Alexandra Nagel, Jessica Starke, Harald Kusch, Oliver Valerius, Kirstin Feussner, Ivo Feussner, Alexander Kaever, Manuel Landesfeind, Burkhard Morgenstern, Doerte Becher, Michael Hecker, Susanna A. Braus-Stromeyer, James W. Kronstad, Gerhard H. Braus
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemical Research Methods
M. Suhr, C. Lehmann, C. R. Bauer, T. Bender, C. Knopp, L. Freckmann, B. Ost Hansen, C. Henke, G. Aschenbrandt, L. K. Kuhlborn, S. Rheinlander, L. Weber, B. Marzec, M. Hellkamp, P. Wieder, U. Sax, H. Kusch, S. Y. Nussbeck
BMC BIOINFORMATICS
(2020)
Article
Plant Sciences
Rebekka Harting, Jessica Starke, Harald Kusch, Stefanie Poeggeler, Isabel Maurus, Rabea Schlueter, Manuel Landesfeind, Ingo Bulla, Minou Nowrousian, Ronnie de Jonge, Gertrud Stahlhut, Katharina J. Hoff, Kathrin P. Asshauer, Andrea Thuermer, Mario Stanke, Rolf Daniel, Burkhard Morgenstern, Bart P. H. J. Thomma, James W. Kronstad, Susanna A. Braus-Stromeyer, Gerhard H. Braus
Summary: The amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize and penetrate plant roots similarly, but Vl43 exhibits higher proliferation and virulence compared to Vl32. Their highly conserved genomes contain less than 1% unique genes, and their karyotypes display changed genetic synteny due to substantial genomic reshuffling. Furthermore, a specific 20 kb region unique to Vl43 is responsible for mediating virulence-reducing functions, limiting damage on the host plant, and preventing Vl43 from becoming even more virulent.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Microbiology
Rebekka Harting, Alexandra Nagel, Kai Nesemann, Annalena M. Hoefer, Emmanouil Bastakis, Harald Kusch, Claire E. Stanley, Martina Stockli, Alexander Kaever, Katharina J. Hoff, Mario Stanke, Andrew J. deMello, Markus Kuenzler, Cara H. Haney, Susanna A. Braus-Stromeyer, Gerhard H. Braus
Summary: Research has shown that different fluorescent pseudomonads have varying effects on fungal growth, with Pseudomonas protegens CHA0 exhibiting the strongest inhibitory potential. Fungal morphology and transcriptome are altered during co-cultivation with pseudomonads, and the bacterial effect on the pathogen contributes to plant protection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Catia Monteiro, Sandra Heinrich, Inka Bartsch, Klaus Valentin, Erwan Corre, Jonas Collen, Lars Harms, Gernot Gloeckner, Kai Bischof
FRONTIERS IN MARINE SCIENCE
(2019)
Article
Cell Biology
Maria Stumpf, Rolf Mueller, Berthold Gassen, Regina Wehrstedt, Petra Fey, Malte A. Karow, Ludwig Eichinger, Gernot Gloeckner, Angelika A. Noegel
DISEASE MODELS & MECHANISMS
(2017)
Article
Biotechnology & Applied Microbiology
Reema Singh, Hajara M. Lawal, Christina Schilde, Gernot Gloeckner, Geoffrey J. Barton, Pauline Schaap, Christian Cole
Article
Biotechnology & Applied Microbiology
Kai Nesemann, Susanna A. Braus-Stromeyer, Rebekka Harting, Annalena Hoefer, Harald Kusch, Alinne Batista Ambrosio, Christian Timpner, Gerhard H. Braus
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)