期刊
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
卷 17, 期 12, 页码 E1255-E1258出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2013.07.016
关键词
OXA-23; NDM-1; European clone II; Carbapenem-resistant Acinetobacter baumannii; Kenya
资金
- National Research Institutes
Background: The emergence of carbapenem-resistant Acinetobacter baumannii (CRAB) was observed in a Kenyan hospital from 2009 to 2010. Further investigation of the dissemination of CRAB isolates and the molecular characterization of associated resistance determinants were therefore performed. Methods: Antibiotic susceptibilities were determined by broth microdilution and Etest. Metallo-beta-lactamases were detected by Etest method. Clonal relationships were studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). beta-Lactam and aminoglycoside resistance determinants and the clonal relatedness to widespread European clones were studied by PCR and sequencing. Results: Sixteen CRAB isolates from 10 patients possessed six pulsotypes; half of the isolates belonged to the European clone II (ECII) lineage. ECII strains were typed as MLST sequence type 2 (ST2) and ST109, and non-ECII strains as ST25 and ST113. All isolates harbored ISAba1-bla(OXA-23), bla(OXA-51-like), bla(ADC), and class 1 integron, including one that also harbored bla(NDM-1). ADC-57 and two integron cassettes (arr-2-cmlA5 and aadB-aadA2-cmlA6-aadA15) were newly-identified. Non-ECII isolates, designated non-ECII clone, carried armA and integron cassette arr-2-cmlA5. Conclusions: Two distinct clones of CRAB - ECII and non-ECII epidemic clones - were disseminated in Kenya. The concomitance of ISAba1-bla(OXA-23) was the major mechanism contributing to CRAB. The first identification of ECII CRAB and New Delhi metallo-beta-lactamase 1 (NDM-1) extensively drug-resistant A. baumannii in East Africa is of concern. (C) 2013 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
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