Article
Biotechnology & Applied Microbiology
Sara Lupacchini, Jens Appel, Ron Stauder, Paul Bolay, Stephan Klaehn, Elisabeth Lettau, Lorenz Adrian, Lars Lauterbach, Bruno Buehler, Andreas Schmid, Joerg Toepel
Summary: This study successfully addressed the challenge of oxygen release during photosynthesis-driven H-2 production by introducing an oxygen-tolerant hydrogenase into cyanobacteria. The hydrogenase enabled the bacteria to utilize H-2 to fix CO2 and produce H-2 under different conditions, laying a foundation for further engineering advancements.
METABOLIC ENGINEERING
(2021)
Article
Chemistry, Physical
Ayshat M. Bozieva, Makhmadyusuf Kh. Khasimov, Roman A. Voloshin, Maria A. Sinetova, Elena V. Kupriyanova, Sergey K. Zharmukhamedov, Dmitry O. Dunikov, Anatoly A. Tsygankov, Tatsuya Tomo, Suleyman I. Allakhverdiev
Summary: This study aims to investigate the H2-releasing ability of new strains of cyanobacteria and evaluate the effects of the photosystem II inhibitor DCMU on H2 production under light and dark conditions. The results demonstrate that cultures treated with DCMU produced several times more H2 than untreated cells. The highest H2 photoproduction rate of 4.24 mmol H2 (mg Chl a h)-1 was observed in Dolichospermum sp. IPPAS B.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Chemistry, Multidisciplinary
Claudia Brocks, Chandan K. Das, Jifu Duan, Shanika Yadav, Ulf-Peter Apfel, Subhasri Ghosh, Eckhard Hofmann, Martin Winkler, Vera Engelbrecht, Lars V. Schaefer, Thomas Happe
Summary: This study proposes a new strategy to improve the O-2 stability of [FeFe]-hydrogenases by blocking the O-2 diffusion pathway and protecting the catalytic cofactor.
Article
Chemistry, Physical
Gulzhanay K. Kamshybayeva, Bekzhan D. Kossalbayev, Asemgul K. Sadvakasova, Meruyert O. Bauenova, Bolatkhan K. Zayadan, Ayshat M. Bozieva, Hesham F. Alharby, Tatsuya Tomo, Suleyman I. Allakhverdiev
Summary: There is a growing interest in biologically derived, inexhaustible energy sources based on solar energy, as there has been a tendency to postpone dealing with climate concerns. Cyanobacteria have the potential to produce clean, renewable fuels in the form of hydrogen gas. A recent study screened 11 cyanobacterial strains for efficient hydrogen production and found that the most active species was Anabaena variabilis BTA-1047, with an H2 production activity of 21.3 mmol mg-1 Chl a h-1. The study also questioned the idea that only an anaerobic environment is required for H2 production by cyanobacterial species.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Microbiology
Franz Opel, Marvin Amadeus Itzenhaeuser, Isabel Wehner, Sara Lupacchini, Lars Lauterbach, Oliver Lenz, Stephan Klahn
Summary: Cyanobacteria have attracted attention in biotechnology for sustainable production of molecular hydrogen (H-2), but face various constraints. Researchers introduced the regulatory [NiFe]-hydrogenase from Cupriavidus necator into the cyanobacterial model strain Synechocystis sp. PCC 6803, providing the basis for the development of an intrinsic H-2 biosensor in cyanobacterial cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Rhiannon M. Evans, Natalie Krahn, Bonnie J. Murphy, Harrison Lee, Fraser A. Armstrong, Dieter Soll
Summary: The study investigates the impact of substituting cysteine with selenocysteine on the function of [NiFe]-hydrogenases, revealing that while the activity of the enzymes is reduced after substitution, variants with selenocysteine show significant differences in O-2 tolerance and electrocatalytic properties.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Chemical
Paulo Jorge Marques Cordeiro-Junior, Cristina Saez Jimenez, Marcos Roberto De Vasconcelos Lanza, Manuel Andres Rodrigo Rodrigo
Summary: This work presents the development and application of a gas diffusion electrode based on Printex L6 carbon for the production of high concentrations of hydrogen peroxide. The Printex L6 carbon-based electrode achieved significantly higher H2O2 concentrations compared to the Vulcan XC-72R carbon-based electrode, and also exhibited high stability.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Review
Energy & Fuels
S. K. Mandotra, Chitra Sharma, N. Srivastava, A. S. Ahluwalia, P. W. Ramteke
Summary: Biohydrogen production from algae holds promise as a clean and sustainable fuel, but faces obstacles such as cost effectiveness, storage, and transportation.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Review
Chemistry, Physical
Gulzhanay K. Kamshybayeva, Bekzhan D. Kossalbayev, Asemgul K. Sadvakasova, Bolatkhan K. Zayadan, Ayshat M. Bozieva, Dmitry Dunikov, Saleh Alwasel, Suleyman I. Allakhverdiev
Summary: Cyanobacteria are potential producers of economically viable hydrogen energy, but the current amount of hydrogen obtained is limited. To produce large amounts of cyanobacterial hydrogen, the most active wild-type strains need to be selected and technological, modular, and genetic research must be conducted simultaneously. The low energy efficiency of cyanobacterial hydrogen also requires comprehensive research.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Engineering, Environmental
Yang Li, Yixin Zhang, Guangshen Xia, Juhong Zhan, Gang Yu, Yujue Wang
Summary: This study evaluated the feasibility of electrochemical hydrogen peroxide (H2O2) production with gas diffusion electrode (GDE) for decentralized water treatment, showing high current efficiency and low energy consumption, making it a promising technology for household and community water treatment.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2021)
Article
Engineering, Environmental
Yang Li, Christopher J. Miller, Lei Wu, T. David Waite
Summary: This study demonstrates an electrochemical advanced oxidation process (EAOP) that can simultaneously catalyze the hydrogen evolution reaction (HER) and the oxygen reduction reaction (ORR) to generate atomic hydrogen (H*) and hydrogen peroxide (H2O2) in situ. By using a palladium-coated carbon-PTFE gas diffusion electrode (Pd/C GDE) as a catalytic cathode, hydroxyl radicals ((OH)-O-center dot) can be formed by the reaction of electrogenerated H* with H2O2. The H*/GDE process is more effective in degrading organic contaminants compared to the conventional H*/H2O2 process.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Michael G. Allan, Thomas Pichon, Jade A. McCune, Christine Cavazza, Alan Le Goff, Moritz F. Kuhnel
Summary: This work demonstrates the performance of [NiFeSe] hydrogenase from Desulfomicrobium baculatum for solar-driven hydrogen generation in a variety of organic-based deep eutectic solvents. It shows that the hydrogenase exhibits remarkable performance under aerobic conditions in these solvents when paired with a TiO2 photocatalyst. The tuning of water content further enhances hydrogen evolution activity and quantum yield. Contrary to common belief, these findings suggest that placing natural hydrogenases in non-natural environments can enhance their intrinsic activity, opening possibilities for full water splitting using hydrogenases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Plant Sciences
John T. Hancock, Grace Russell
Summary: Molecular hydrogen (H2) is considered part of the small molecules that can control cellular activity, potentially used in human disease therapy and plant growth enhancement. Its molecular action is still unclear, but it may act through antioxidative effects or by influencing cell antioxidation capacity, with possible interactions with heme oxygenase activity or reactive nitrogen species. However, there is controversy surrounding the suggested mechanisms, with downstream mechanisms still needing critical review.
Article
Chemistry, Physical
Nuttavut Kosem, Motonori Watanabe, Jun Tae Song, Atsushi Takagaki, Tatsumi Ishihara
Summary: The photobiocatalytic system for H2 production was investigated in terms of the rational characteristics of biocatalysts and the role of individual components. It was found that tris (2-amino-2-hydroxymethyl-1,3-propanediol) acted as an ideal electron donor for viologen reduction by TiO2. The activity of whole-cell biocatalysts depended on the cell permeability, the redox potential of electron mediators, and the cell envelope.
APPLIED CATALYSIS A-GENERAL
(2023)
Review
Microbiology
Simone Morra
Summary: FeFe-hydrogenases are complex metalloenzymes that play a key role in microbial energy metabolism. They use protons from water as terminal electron acceptors to dissipate excess reducing equivalents and produce hydrogen. FeFe-hydrogenases also have additional physiological functions, such as H-2 uptake, H-2 sensing, and CO2 fixation. Recent advancements in identifying and characterising novel FeFe-hydrogenases have expanded our understanding of their multiple roles and mechanisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Lauri Nikkanen, Anita Santana Sanchez, Maria Ermakova, Matthias Roegner, Laurent Cournac, Yagut Allahverdiyeva
Letter
Plant Sciences
Adrien Burlacot, Yonghua Li-Beisson, Gilles Peltier
Article
Agriculture, Multidisciplinary
Olivier Roupsard, Alain Audebert, Adama P. Ndour, Cathy Clermont-Dauphin, Yelognisse Agbohessou, Josias Sanou, Jonas Koala, Emile Faye, Diaretou Sambakhe, Christophe Jourdan, Guerric le Maire, Laure Tall, Diaminatou Sanogo, Josiane Seghieri, Laurent Cournac, Louise Leroux
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2020)
Article
Agriculture, Multidisciplinary
A. Tounkara, C. Clermont-Dauphin, F. Affholder, S. Ndiaye, D. Masse, L. Cournac
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2020)
Review
Agronomy
Papa Mamadou Sitor Ndour, Thierry Heulin, Wafa Achouak, Laurent Laplaze, Laurent Cournac
Article
Multidisciplinary Sciences
Yandu Lu, Qinhua Gan, Masakazu Iwai, Alessandro Alboresi, Adrien Burlacot, Oliver Dautermann, Hiroko Takahashi, Thien Crisanto, Gilles Peltier, Tomas Morosinotto, Anastasios Melis, Krishna K. Niyogi
Summary: The study found that LHCR proteins play a key role in red algae, affecting the balance between photoprotection and light harvesting in photosynthesis. The mutant hlr1 shows increased tolerance to high light due to reduced oxidative stress in PSI, limiting oxidative damage and promoting growth. However, under low light conditions, HLR1 deficiency reduces PSI light-harvesting capacity and growth.
NATURE COMMUNICATIONS
(2021)
Article
Agronomy
Papa Mamadou Sitor Ndour, Cheikh Mbacke Barry, Diame Tine, Carla De la Fuente Canto, Mariama Gueye, Mohamed Barakat, Philippe Ortet, Wafa Achouak, Ibrahima Ndoye, Bassirou Sine, Laurent Laplaze, Thierry Heulin, Laurent Cournac
Summary: This study demonstrates that in pearl millet, the rhizosheath size is related to soil nutrient dynamics and microbiota diversity. However, it also shows that other factors shape this trait and their relative importance must be determined.
Article
Multidisciplinary Sciences
D. Sorigue, K. Hadjidemetriou, S. Blangy, G. Gotthard, A. Bonvalet, N. Coquelle, P. Samire, A. Aleksandrov, L. Antonucci, A. Benachir, S. Boutet, M. Byrdin, M. Cammarata, S. Carbajo, S. Cuine, R. B. Doak, L. Foucar, A. Gorel, M. Grunbein, E. Hartmann, R. Hienerwadel, M. Hilpert, M. Kloos, T. J. Lane, B. Legeret, P. Legrand, Y. Li-Beisson, S. L. Y. Moulin, D. Nurizzo, G. Peltier, G. Schiro, R. L. Shoeman, M. Sliwa, X. Solinas, B. Zhuang, T. R. M. Barends, J-P Colletier, M. Joffre, A. Royant, C. Berthomieu, M. Weik, T. Domratcheva, K. Brettel, M. H. Vos, I Schlichting, P. Arnoux, P. Mueller, F. Beisson
Summary: The study found that Fatty Acid Photodecarboxylase (FAP) exhibits efficient decarboxylation activity, primarily achieved through the reduction of the flavin chromophore and direct removal of carbon dioxide by the substrate carboxyl group. Essential residues R451 and C432 are crucial for substrate stabilization and charge transfer.
Article
Agriculture, Multidisciplinary
Matthew B. H. Bright, Ibrahima Diedhiou, Roger Bayala, Nathaniel Bogie, Lydie Chapuis-Lardy, Teamrat A. Ghezzehei, Christophe Jourdan, Donatien Moucty Sambou, Yacine Badiane Ndour, Laurent Cournac, Richard P. Dick
Summary: Intercropping with Guiera senegalensis can significantly increase crop yields, notably for millet where yields increased by an average of 126% across various fertilizer treatments. This system maintained yields in low rainfall years and had greater water use efficiency for both millet and groundnut. These responses were associated with improved soil quality, including increased levels of particulate and total organic matter, and extractable nutrients.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2021)
Article
Agronomy
Adrien Byamungu Ndeko, Hassna Founoune-Mboup, Aboubacry Kane, Laurent Cournac
Summary: The study revealed that AMF temperature stress reduces mycorrhizal colonization rate, plant biomass, and soil aggregation of pearl millet lines, but inoculation with AMF positively impacts the tolerance of pearl millet lines to temperature stress.
Review
Plant Sciences
Ousmane Dao, Franziska Kuhnert, Andreas P. M. Weber, Gilles Peltier, Yonghua Li-Beisson
Summary: This review discusses the functions of malate shuttles in various biological processes and emphasizes recent research findings and the similarities and differences in different organisms, providing an evolutionary perspective on redox trafficking in photosynthetic eukaryotes.
TRENDS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Adrien Burlacot, Ousmane Dao, Pascaline Auroy, Stephan Cuine, Yonghua Li-Beisson, Gilles Peltier
Summary: Global photosynthesis consumes ten times more CO2 than net anthropogenic emissions, and microalgae account for nearly half of this consumption. In the green alga Chlamydomonas reinhardtii, cyclic electron flow and O-2 photoreduction generate a low luminal pH essential for CO2 concentration, while electron flow from chloroplast to mitochondria energizes inorganic carbon transport.
Article
Multidisciplinary Sciences
C. de la Fuente Canto, M. N. Diouf, P. M. S. Ndour, M. Debieu, A. Grondin, S. Passot, A. Champion, C. Barrachina, M. Pratlong, P. Gantet, K. Assigbetse, N. Kane, P. Cubry, A. G. Diedhiou, T. Heulin, W. Achouak, Y. Vigouroux, L. Cournac, L. Laplaze
Summary: This study investigated the correlation between root-adhering soil mass and root hair development, root architecture, and symbiosis with arbuscular mycorrhizal fungi in pearl millet. The genetic control of this trait was analyzed using genome wide association (GWAS) and gene expression studies. The results showed that root exudation mainly regulates rhizosheath formation, which is under complex genetic control in pearl millet.
SCIENTIFIC REPORTS
(2022)
Article
Soil Science
M. Oscar Kisaka, Chris Shisanya, Laurent Cournac, J. Raphael Manlay, Harun Gitari, Jonathan Muriuki
Summary: Conservation agriculture with trees (CAWT) is a beneficial strategy for improving soil quality in smallholder farming. This study identified key soil quality indicators under CAWT and assessed the effects of different components on soil quality. The results have important implications for maize production.
SOIL & TILLAGE RESEARCH
(2023)
Article
Plant Sciences
Solene L. Y. Moulin, Audrey Beyly-Adriano, Stephan Cuine, Stephanie Blangy, Bertrand Legeret, Magali Floriani, Adrien Burlacot, Damien Sorigue, Poutoum-Palakiyem Samire, Yonghua Li-Beisson, Gilles Peltier, Fred Beisson
Summary: Fatty acid photodecarboxylase (FAP) is a photoenzyme that requires light for catalytic cycle, and recent studies have shown its conservation and role in the formation of hydrocarbons in algae such as Chlamydomonas reinhardtii. This enzyme is predominantly membrane-associated and its activity is linked to photosynthetic membranes, suggesting a potentially important role in the evolution and function of algal lineages.
