Article
Biotechnology & Applied Microbiology
Xueqin Lv, Ke Jin, Yu Yi, Lingang Song, Xiang Xiu, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Long Liu
Summary: By visual observation and order analysis of membrane microdomains of Saccharomyces cerevisiae, it was found that membrane microdomains play a crucial role in acid tolerance. This study proposed a new acid tolerance mechanism, providing a new direction for the rational engineering of acid-tolerant strains.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Zedi Wang, Linlin Zhou, Minrui Lu, Yuwei Zhang, Samina Perveen, Huarong Zhou, Zhiqiang Wen, Zhaoxian Xu, Mingjie Jin
Summary: Yarrowia lipolytica strain was evolved through adaptive laboratory evolution to increase tolerance to ferulic acid, leading to a new strain with improved viability under high concentrations of ferulic acid, showing promise for application in lignocellulose conversion.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jincai Shi, Boyu Zhao, Shuang Zheng, Xiaowei Zhang, Xiaolin Wang, Wentao Dong, Qiujin Xie, Gang Wang, Yunping Xiao, Fan Chen, Nan Yu, Ertao Wang
Summary: Most land plants acquire phosphorus from soil through direct phosphate uptake pathway via root hairs and epidermis, or through an indirect pathway via mycorrhizal symbiosis. The gene regulatory network between transcription factors and mycorrhizal symbiosis-related genes is controlled by the conserved P-sensing pathway centered on phosphate starvation response (PHR) transcription factors. SPX-domain proteins and OsPHR2 play crucial roles in regulating symbiosis-related genes during mycorrhizal infection.
Article
Biotechnology & Applied Microbiology
Dania Sandoval-Nunez, Teresa Romero-Gutierrez, Carolina Gomez-Marquez, Anne Gshaedler, Melchor Arellano-Plaza, Lorena Amaya-Delgado
Summary: In this study, K. marxianus SLP1 was adapted to increase its tolerance to a mix of inhibitory compounds. The adapted strain, K. marxianus P8, showed improved fermentation and physiological parameters and activated adaptive prediction mechanism through changes in gene expression. These findings suggest that K. marxianus has rapid adaptability to stressful environments and has potential for lignocellulosic ethanol production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Charles Wickham-Smith, Naglis Malys, Klaus Winzer
Summary: This study evolved C. necator to tolerate high concentrations of CO, both heterotrophically and autotrophically. Mutations in the genes cydA2B2 and hoxH were identified, and when engineered into the parental strain, they conferred high CO tolerance under both heterotrophic and autotrophic conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Correction
Biology
Jianfeng Wang, Wenpeng Hou, Michael J. Christensen, Chao Xia, Tao Chen, Zhixin Zhang, Zhibiao Nan
Summary: In the results, there was a mistake in describing the information of Figure 9B, which should be corrected to explain that factor 1 and factor 2 accounted for 71.30% and 16.68% of the total variance, respectively. The 0 mmol L-1 NaCl concentration made the highest contribution to factor 1 in roots of E+ plants, while the 200 mmol L-1 NaCl concentration was significantly loaded on factor 2 in roots of E+ plants.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Zhaoxia Cui, Yuan Liu, Jianbo Yuan, Xiaojun Zhang, Tomer Ventura, Ka Yan Ma, Shuai Sun, Chengwen Song, Dongliang Zhan, Yanan Yang, Hourong Liu, Guangyi Fan, Qingle Cai, Jing Du, Jing Qin, Chengcheng Shi, Shijie Hao, Quinn P. Fitzgibbon, Gregory G. Smith, Jianhai Xiang, Tin-Yam Chan, Min Hui, Chenchang Bao, Fuhua Li, Ka Hou Chu
Summary: The study presents a chromosome-level genome and transcriptome data for the Chinese mitten crab, shedding light on the biology of Brachyurans. The research reveals the expansion of specific gene families in crabs, and highlights the importance of down-regulation of Hox genes during brachyurization development.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Eleftheria Papadopoulou, Mari Cristina Rodriguez de Evgrafov, Argyro Kalea, Panagiotis Tsapekos, Irini Angelidaki
Summary: Three bacterial species were isolated from seaweed biomass and evolved to increasing concentrations of NaCl. Two of the evolved strains showed increased salt tolerance and were able to produce lactic acid in hypersaline seaweed hydrolysate. Mutations in genes affecting ion balance and cell membrane composition were observed in the evolved lineages.
Article
Engineering, Marine
Yuquan Li, Zhihao Zhang, Zhongkai Wang, Zhitong Deng, Ruiyang Zhao, Jinfeng Sun, Pengyuan Hao, Long Zhang, Xiaofan Wang, Fei Liu, Renjie Wang, Yanting Cui
Summary: This study cloned and confirmed ORF sequences of ion-transportation-related genes in Kuruma shrimp, analyzed their tissue expression patterns, and assessed their mRNA expression levels during exposure to different salinity levels. The results suggest that these genes play critical roles in the adaptation of aquatic crustaceans to changes in environmental salinity.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Plant Sciences
Dmitrii A. Matalin, Dmitrii E. Khramov, Alexey V. Shuvalov, Vadim S. Volkov, Yurii V. Balnokin, Larissa G. Popova
Summary: The microalga genus Dunaliella has species with wide salinity tolerance, but the mechanism of excess Na+ removal in cells is still debated. Through studying the expression of DmHA1 and DmHA2 under hyperosmotic salt shock, it was found that DmHA2 plays a crucial role in overcoming salt stress.
Article
Chemistry, Multidisciplinary
Vivekananda Bal, Baron Peters
Summary: The study investigates the impact of periodic stirrer speed oscillations on crystal size distribution in a mixed suspension mixed product removal (MSMPR) crystallizer. It is found that changes in stirring rate at different frequencies have varying effects on crystal growth.
CRYSTAL GROWTH & DESIGN
(2021)
Article
Biotechnology & Applied Microbiology
Yuman Gan, Meng Bai, Xiao Lin, Kai Liu, Bingyao Huang, Xiaodong Jiang, Yonghong Liu, Chenghai Gao
Summary: In this study, we successfully engineered a mutant strain with enhanced macrolactins production using a self-made device and adaptive laboratory evolution technology. The mutant strain showed saline resistance and increased macrolactins production within 60 days. Amino acid metabolism was found to be involved in the production of macrolactins in the evolved strain. The identified hisD mutation played a crucial role in the improvement of macrolactins production. This research provides insights for future metabolic engineering strategies to improve the production of other antibiotics and toxic compounds.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Depeng Zhang, Zhenyue Zhang, Chunxin Li, Yimei Xing, Yaqin Luo, Xinsheng Wang, Donghuan Li, Zhiyun Ma, Hua Cai
Summary: Rare cold-inducible 2 (RCI2) genes from alfalfa are important for regulating ion homeostasis and stabilizing membranes under various abiotic stresses. This study found that the expression patterns and subcellular localization of MsRCI2D and MsRCI2E genes in alfalfa differed under salt, alkali, and ABA treatments. Transgenic alfalfa chimeras overexpressing MsRCI2D showed better phenotype and enhanced salt tolerance compared to those overexpressing MsRCI2E, indicating the functional importance of MsRCI2D in maintaining ion balance and reducing oxidative damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Applied
Haishuo Ji, Lei Wan, Yanxin Gao, Ping Du, Wenjin Li, Hang Luo, Jiarui Ning, Yingying Zhao, Huangwei Wang, Lixin Zhang, Liyun Zhang
Summary: Hydrogenase is an efficient biocatalyst for hydrogen production and utilization, but its poor oxygen tolerance and low yield hinder its practical application. Recent research has revealed the structures, mechanisms, and proton-coupled electron transfer schemes of hydrogenases, allowing for their coupling with other catalysts to build novel reactors. This review summarizes the efforts made by scientists to address these challenges and design novel reactors in the field of hydrogenase-based applications.
JOURNAL OF ENERGY CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Haiqi Fu, Xiang Yu, Yuanyuan Jiang, Yuhan Wang, Yongqing Yang, She Chen, Qijun Chen, Yan Guo
Summary: In this study, it was found that SOS3-LIKE CALCIUM-BINDING PROTEIN8 (SCaBP8) mediates the regulation of phosphatases PP2C.D6 and PP2C.D7 by calcium signaling, which inhibits the activity of sodium ion/hydrogen ion antiporter SOS1 under non-salt-stress conditions. Under salt stress, SCaBP8 interacts with PP2Cs and suppresses their phosphatase activity, while also regulating the subcellular localization of PP2C.D6. These findings reveal the negative regulatory mechanism of the SOS pathway and provide important clues for further research on plant salt tolerance.
