期刊
MICROGRAVITY SCIENCE AND TECHNOLOGY
卷 28, 期 3, 页码 247-260出版社
SPRINGER
DOI: 10.1007/s12217-015-9456-7
关键词
Thyroid cells; Simulated microgravity; Random Positioning Machine; Clinostat; Cytokines; Spheroids
资金
- European Space Agency (ESA-CORA-GBF-PROJECT) [2011-005, 2013-001]
- German Space Administration (DLR
- BMWi) [50WB1124/50WB1524]
- DGLRM
The ground-based facilities 2D clinostat (CN) and Random Positioning Machine (RPM) were designed to simulate microgravity conditions on Earth. With support of the CORA-ESA-GBF program we could use both facilities to investigate the impact of simulated microgravity on normal and malignant thyroid cells. In this review we report about the current knowledge of thyroid cancer cells and normal thyrocytes grown under altered gravity conditions with a special focus on growth behaviour, changes in the gene expression pattern and protein content, as well as on altered secretion behaviour of the cells. We reviewed data obtained from normal thyrocytes and cell lines (two poorly differentiated follicular thyroid cancer cell lines FTC-133 and ML-1, as well as the normal thyroid cell lines Nthy-ori 3-1 and HTU-5). Thyroid cells cultured under conditions of simulated microgravity (RPM and CN) and in Space showed similar changes with respect to spheroid formation. In static 1g control cultures no spheroids were detectable. Changes in the regulation of cytokines are discussed to be involved in MCS (multicellular spheroids) formation. The ESA-GBF program helps the scientists to prepare future spaceflight experiments and furthermore, it might help to identify targets for drug therapy against thyroid cancer.
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