期刊
MICROBIOLOGY AND IMMUNOLOGY
卷 59, 期 6, 页码 357-364出版社
WILEY-BLACKWELL
DOI: 10.1111/1348-0421.12264
关键词
cryptococcal meningitis; peripheral blood mononuclear cell; Toll-like receptors 2
资金
- 973 Foundation [2013CB531606]
- National Science Foundation of China [81273282, 81202353, 81371786, 81302579]
- Shanghai Municipal Commission for Science and Technology [11JC1410902]
- Changhai Hospital [CH125530300]
- Nanjing District [12MA056]
- Educational Reform Fund of the Second Military Medical University
- Master's Fund of the Second Military Medical University graduate school
Toll-like receptors are the most important pattern recognition receptors that can recognize conserved molecular structures shared by large groups of pathogens. Here, the aim was to determine the expression and role of TLR2 in peripheral blood mononuclear cells (PBMCs) from patients with cryptococcal meningitis and healthy controls. TLR2 expression was measured using RT-PCR and western blotting. The role of TLR2 in cytokine production by PBMCs after Cryptococcus neoformans exposure was assessed in healthy controls prior to incubation with anti-TLR2. TLR2 mRNA and protein expression were both weaker in patients with cryptococcal meningitis than in healthy controls. Furthermore, pre-incubation of PBMCs from healthy donors with anti-TLR2 led to reduced expression of IFN- and IL-12p70, but not of IL-4 and IL-10, following C. neoformans stimulation. Our results suggest that impaired expression of TLR2 may be involved in defective host defense to C. neoformans through an attenuated Th1 response.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据