Article
Engineering, Biomedical
Yuksel Cetin, Merve G. Sahin, Fatma N. Kok
Summary: This study evaluated the cardiomyocyte differentiation potential of human adipose mesenchymal stem cells on silk fibroin scaffolds for cardiac tissue engineering. The combination showed promising results in terms of cardiac regeneration and repair, making it a potential tool for future applications in the field.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
A. Dadashzadeh, S. Moghassemi, C. A. Amorim
Summary: This study demonstrated that PEGylated fibrin hydrogels enhanced scaffold stability, supported cell viability and proliferation. PEG:Fib 5:1 T50 showed a significantly higher cell density dynamic and a lower expression of caspase-3. In contrast, PEG:Fib 10:1 T50 hydrogels had uniform cell distribution and a higher rate of Ki67-positive cells.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Katsuhisa Sakaguchi, Yusuke Tobe, Jiayue Yang, Ryu-ichiro Tanaka, Kumiko Yamanaka, Jiro Ono, Tatsuya Shimizu
Summary: The article introduces a novel tissue engineering method using a net metal mould without the use of a scaffold, which allows for the formation of 3D tissues by culturing human dermal fibroblasts. The study successfully constructs scaffold-free 3D tissues with controllable strength, which may have a significant impact on next-generation tissue engineering products.
Article
Materials Science, Multidisciplinary
Zihao Jia, Yang Liu, Yingying Wang, Shiyuan Peng, Peng Jia, Wei Zhang, Xiaoyan Tan
Summary: Gas-foaming technique can transform two-dimensional scaffolds into three-dimensional scaffolds, which have shown better cartilage regeneration effects with larger pore size, higher porosity, and higher biocompatibility.
MATERIALS RESEARCH EXPRESS
(2021)
Article
Chemistry, Physical
Yujie Chen, Wei Xu, Muhammad Shafiq, Jincheng Tang, Junxiang Hao, Xianrui Xie, Zhengchao Yuan, Xianghao Xiao, Yu Liu, Xiumei Mo
Summary: This study successfully fabricated two-dimensional and three-dimensional electrospun poly(L-lactide-co-epsilon-caprolactone)/silk fibroin scaffolds with nanofibrous structure and biological function, further mimicking the microarchitecture of native cartilage through crosslinking. The three-dimensional scaffolds showed better mechanical properties, while the biocompatibility, chondrocyte proliferation, and phenotypic maintenance were superior in the three-dimensional scaffolds as well.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Ji Min Seok, Goeun Choe, Sang Jin Lee, Min-Ah Yoon, Kwang-Seop Kim, Jun Hee Lee, Wan Doo Kim, Jae Young Lee, Kangwon Lee, Su A. Park
Summary: The study demonstrates that using an ecofriendly mussel-inspired GO coating can effectively enhance the functionality and bioactivity of 3D-printed scaffolds. By regulating the amount of GO deposition and employing a non-toxic coating method, the surface properties are improved, aiding in promoting osteogenesis.
MATERIALS & DESIGN
(2021)
Article
Materials Science, Multidisciplinary
Pedram Iranmanesh, Mohammad Gowdini, Abbasali Khademi, Mohammad Dehghani, Mehran Latifi, Naif Alsaadi, Mehran Hemati, Ramin Mohammadi, Saeed Saber-Samandari, Davood Toghraie, Afrasyab Khan
Summary: The combination of 3D bioprinting technology with novel hydrogel and bioink has led to the successful fabrication of scaffolds with unique mechanical and biological responses. The addition of naproxen coating enhances the tensile strength of the scaffolds, showing promising compatibility with the human body.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Engineering, Biomedical
Marie Cuvellier, Sophie Rose, Frederic Ezan, Ulrich Jarry, Hugo de Oliveira, Arnaud Bruyere, Christophe Drieu La Rochelle, Vincent Legagneux, Sophie Langouet, Georges Baffet
Summary: In recent decades, the development of 3D in vitro cultures of primary human hepatocytes (PHHs) has become increasingly important for mimicking liver functions. This study utilized 3D bioprinting technology to print PHH in a GelMa matrix, resulting in the rapid organization of polarized hollow spheroids that remained viable for at least 28 days. The bioprinted PHH exhibited high differentiation and maintained liver-specific functions over time, including the activities of phase I and II biotransformation enzymes. The study also demonstrated the ability of the bioprinted structures to vascularize when implanted in mice. This model shows promise for generating human liver tissue and advancing biotechnological developments.
Article
Biochemistry & Molecular Biology
Zhinan Mao, Xuewei Bi, Fan Ye, Puyu Du, Xiong Shu, Lei Sun, Juan Guan, Xiaoming Li, Sujun Wu
Summary: The appropriate dosage of crosslinking agent EGDE is critical for achieving balanced mechanical properties, in vivo degradability, and immunological properties of silk fibroin scaffold platform for soft tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Yuqing Niu, Massimiliano Galluzzi, Fuming Deng, Zhang Zhao, Ming Fu, Liang Su, Weitang Sun, Wei Jia, Huimin Xia
Summary: The study found that the hyaluronic acid-coating silk fibroin nanofiber scaffold can effectively regulate the epithelialization process of urethral regeneration, promoting luminal epithelialization, smooth muscle bundle structural remodeling, and capillary formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Nathan V. Roblin, Megan K. DeBari, Sandra L. Shefter, Erica Iizuka, Rosalyn D. Abbott
Summary: Concerns for the environment have led to the development of eco-friendly approaches to fabricating biomaterials, particularly in silk fibroin scaffold production. In this study, an integrated green fibroin scaffold approach using sodium hydroxide instead of sodium carbonate for degumming and an aqueous-based alternative for fabrication was characterized. The environmentally friendly scaffolds demonstrated similar properties to traditional scaffolds, with increased porosity and cell seeding density. The methodology was found to be suitable for soft tissue applications, with high viability of human adipose-derived stem cells and functional adipocytes within the scaffolds.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Sophia Szymkowiak, Nathan Sandler, David L. Kaplan
Summary: Chronic kidney disease and kidney failure are increasing globally, with a lack of corresponding improvement in available therapies. Using silk fibroin protein scaffolds to support kidney cells can mimic the structure of the kidney and support a larger number of cells with more even distribution under perfused conditions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Yunsheng Dong, Hui Xiao, Jie Wang, Tingting Yang, Naiqi Kang, Jiaxing Huang, Wei Cui, Yufei Liu, Qiang Yang, Shufang Wang
Summary: Constructing scaffolds with osteogenic activity through tissue engineering is an effective strategy for bone regeneration. This study used gelatin, silk fibroin, and Si3N4 to prepare silicon-functionalized bio-macromolecules composite scaffolds using 3DP technology. The scaffold showed good cyto-compatibility and promoted osteogenic differentiation of MSCs in vitro, and facilitated bone regeneration in vivo. The composite scaffold system has great potential for application in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Multidisciplinary
Kun Li, Yingnan Zhang, Junwei Xu, Jingxi Wang, Xuenan Gu, Ping Li, Yubo Fan
Summary: The magnetic PCL/Fe3O4/ICA 3D scaffold, manufactured through electrospinning, exhibited enhanced cell proliferation and infiltration, especially when combined with the static magnetic field, providing a new idea for the design and application of magnetic scaffolds in bone tissue engineering.
COMPOSITES PART B-ENGINEERING
(2021)
Review
Pharmacology & Pharmacy
Constancio Gonzalez-Obeso, Emily Jane Hartzell, Ryan Albert Scheel, David L. Kaplan
Summary: Effective drug delivery is crucial for successful medical treatment. Silks and recombinant silks have emerged as preferred polymeric drug delivery systems due to their unique properties and tailored physicochemical characteristics.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Materials Science, Multidisciplinary
Shengfei Zhou, Kai Jin, Talia Khan, Zaira Martin-Moldes, David L. Kaplan, Markus J. Buehler
Summary: More complex bioadhesives were investigated, and the physical properties of the adhesive were improved through cold denaturation and swelling. This resulted in enhanced mechanical performance and water resistance of wood composites.
ADVANCED ENGINEERING MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Sabrina C. Shen, Eesha Khare, Nicolas A. Lee, Michael K. Saad, David L. Kaplan, Markus J. Buehler
Summary: Engineered materials are important for modern technology but often contribute to ecological deterioration. Next-generation materials can address sustainability goals by providing alternatives to fossil fuel-based materials and reducing extraction processes and solid waste. Challenges include investigating and designing new feedstocks, which are mechanically weak and difficult to standardize. This review outlines a framework for examining sustainability in material systems and discusses the role of computational tools in discovering novel sustainable materials, with a focus on bioinspired and biobased materials.
Article
Multidisciplinary Sciences
Maobin Xie, Liming Lian, Xuan Mu, Zeyu Luo, Carlos Ezio Garciamendez-Mijares, Zhenrui Zhang, Arturo Lopez, Jennifer Manriquez, Xiao Kuang, Junqi Wu, Jugal Kishore Sahoo, Federico Zertuche Gonzalez, Gang Li, Guosheng Tang, Sushila Maharjan, Jie Guo, David L. Kaplan, Yu Shrike Zhang
Summary: This study demonstrates the rapid volumetric printing of unmodified silk sericin and silk fibroin inks, which exhibit shape-memory and tuneable mechanical properties. The inks are cytocompatible, providing expanded bioink libraries for volumetric additive manufacturing and offering potential for diverse biomedical applications.
NATURE COMMUNICATIONS
(2023)
Review
Cell Biology
Elizabeth K. Johnston, Rosalyn D. Abbott
Summary: Obesity is a growing problem, with a significant number of Americans being obese or morbidly obese. Tissue remodeling and changes in cellular populations and phenotypes occur as a result of pathological tissue expansion. This leads to local and systemic effects, including diabetes and cardiovascular disease. Vascular dynamics play a role in metabolic dysfunction during obesity progression. This review focuses on paracrine, autocrine, and matrix-dependent signaling between adipocytes and endothelial cells in the development and progression of obesity and related diseases.
Article
Materials Science, Multidisciplinary
Jaewon Choi, Jugal Kishore Sahoo, Onur Hasturk, Thomas Falcucci, Ya Yao, David L. Kaplan
Summary: This study presents a new strategy to fabricate silk protein-based aerosols and silk fibers instantaneously in situ using a spray device, without the need for complicated and costly advanced manufacturing techniques. The key to success lies in the conformational transition of silk fibroin from random coil to beta-sheet before spraying, achieved by mixing silk and polyethylene glycol solutions in the spray device. The system utilizes green solvents and shear and elongational flow to provide a portable and user-friendly method for drug delivery carriers and surface coatings.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Applied
Qiongling Chen, Jinchuang Zhang, Yujie Zhang, Haodong Liu, Tongqing Li, Qiang Wang, David L. Kaplan
Summary: This study investigated the interactions between pea protein and fatty acids as well as the effect on protein conformational changes during high-moisture extrusion processing. The results showed that fatty acids induced the exposure of hydrophobic groups, weakened hydrogen bonds, and affected the aggregation of protein subunits. Unsaturated fatty acids limited protein chain refolding and covalent interactions. Additionally, stearic acid promoted the formation of fibrous structures.
Review
Engineering, Biomedical
Rebecca Donahue, Jugal Kishore Sahoo, Sara Rudolph, Ying Chen, David L. Kaplan
Summary: Mucus is a hydrogel that protects the body's surfaces and plays a role in defense against pathogens. Dysfunction of the gut mucus barrier is associated with diseases. Current methods for studying mucus have limitations. Therefore, there is a need for mucus-mimetic hydrogels that accurately reflect the human epithelial environment for research on disease and microbiome interactions.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Jugal Kishore Sahoo, Onur Hasturk, Thomas Falcucci, David L. Kaplan
Summary: Silk fibroin is widely used in various medical fields such as tissue engineering, regenerative medicine, drug delivery, and medical devices. Advancements in silk chemistry and biomaterial designs have resulted in exciting tools for creating new silk-based materials and technologies. Selective chemistries can enhance or modify the properties of silk to address challenges in medically relevant materials.
NATURE REVIEWS CHEMISTRY
(2023)
Article
Engineering, Biomedical
Adam S. Mullis, David L. Kaplan
Summary: Aging-associated neurodegenerative diseases, like Alzheimer's and Parkinson's diseases, are still not well understood and lack effective treatments. Current in vitro and in vivo models cannot fully replicate the human brain tissue structure, function, and pathobiology, leading to translational failures. Bioengineered tissue culture models offer promising alternatives by overcoming the limitations of existing models and providing a better mimic of human brain tissues. In this article, we discuss various bioengineering techniques and design considerations for generating neurodegenerative tissue models, as well as approaches to incorporate cellular diversity and simulate aging processes.
Article
Green & Sustainable Science & Technology
Kirsten R. Trinidad, Reina Ashizawa, Amin Nikkhah, Cameron Semper, Christian Casolaro, David L. Kaplan, Alexei Savchenko, Nicole Tichenor Blackstone
Summary: Growth factors are critical components in cultivated meat production, but their environmental impacts have not been accurately quantified in current assessments. This study conducted a life cycle assessment to evaluate the environmental impacts of recombinant growth factor production for cultivated meat applications. The results show that even in small quantities, recombinant growth factors can have significant environmental impacts. Future research should explore sustainable production methods or alternative options with lower environmental impacts.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Polymer Science
Onur Hasturk, Jugal Kishore Sahoo, David L. Kaplan
Summary: This study demonstrates the synthesis of a hybrid biopolymer composed of silk and poly(guluronate) for on-demand and reversible stiffening and softening of silk-based hydrogels. The hybrid polymers display dynamic morphology and mechanics, as well as proteolytic degradation properties. They also support cell attachment and survival, making them potentially useful in cell culture and disease modeling.
Article
Engineering, Electrical & Electronic
Atul Sharma, Cihan Asci, Ruben Del-Rio-Ruiz, Kirsten Trinidad, Nafize Ishtiaque Hossain, David L. Kaplan, Sameer Sonkusale
Summary: A portable and scalable multiplexed thread-based sensing microprobe is introduced for continuous monitoring of pH and ammonium ions during the biofermentation process. The microprobe consists of a 3D-printed shell to hold separate working and reference electrode threads, allowing for continuous flow during monitoring. The functionality of the microprobe is evaluated in a simulated microreactor resembling a fermentation environment, showing promising dynamic range for pH and ammonium ions in bioreactor monitoring.
IEEE SENSORS LETTERS
(2023)
Article
Materials Science, Biomaterials
Valeria E. Bosio, Christofer Rybner, David L. Kaplan
Summary: The development of 3D-tissue models for studying bone physiology and disease using silk fibroin (SF) faces challenges. To improve mechanical properties and mineralization ability, concentric mineralized SF-based scaffolds were developed. In simulated bone regeneration experiments, hybrid SF with silica microparticles or calcium carbonate nano-structured microparticles showed enhanced cell infiltration, compressive strength, expression of osteogenic markers, and mineralization.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biochemical Research Methods
K. Ramki, G. Thiruppathi, Selva Kumar Ramasamy, P. Sundararaj, P. Sakthivel
Summary: A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semiaqueous solution. The probe exhibited enhanced fluorescence in its aggregated state and even higher fluorescence when chelated with Hg(II). The probe demonstrated high sensitivity and specificity for Hg(II) detection and was successfully applied for imaging Hg(II) in a living model.
Article
Biochemical Research Methods
Qun Zhang, Rui Yang, Gang Liu, Shiyan Jiang, Jiarui Wang, Juqiang Lin, Tingyin Wang, Jing Wang, Zufang Huang
Summary: This research aims to develop a cost-effective and portable method for measuring creatinine levels using the enhanced Tyndall effect phenomenon. The method offers a promising solution for monitoring renal healthcare in resource-limited settings.