期刊
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
卷 56, 期 10-12, 页码 833-839出版社
U B C PRESS
DOI: 10.1387/ijdb.120136pw
关键词
ovary; oocyte; follicle; zona pellucida; mZP1; mZP2; mZP3; null mutant; fertility
资金
- Hoffmann-La Roche Inc
- National Institutes of Health (NICHD)
All mammalian eggs are surrounded by a relatively thick extracellular coat, called the zona pellucida (ZP), that plays vital roles during oogenesis, fertilization, and preimplantation development.The mouse egg ZP consists of three glycoproteins, called mZP1-3, that are synthesized solely by oocytes during their 2-to-3 week growth phase. The ZP is seen initially as isolated extracellular deposits of nascent ZP fibrils that coalesce to form a thickening matrix. Elimination of ZP glycoprotein synthesis by targeted mutagenesis yields mice that are heterozygous or homozygous for the null mutations. Homozygous null males are unaffected by the mutations and heterozygous females are as fertile as wild-type females. On the other hand, eggs from mZP2-/- and mZP3-/- females lack a ZP and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts. Development of ovarian follicles is retarded in homozygous null females and manifested as reduced ovarian weight, number of Graafian follicles, and number of ovulated eggs. Eggs from mZP1-/- females have a distorted ZP and, although the mice are fertile, they exhibit reduced fertility due to early embryonic loss. Potential relationships between ZP biogenesis, folliculogenesis, and fertility are addressed.
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