期刊
METABOLIC ENGINEERING
卷 29, 期 -, 页码 189-195出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2015.03.015
关键词
PHB; P3HP; P3HB3HP; Synthetic biology; Escherichia coli
资金
- State Basic Science Foundation 973 [2012CB725201]
- National High Tech 863 [2012AA023102]
- National Natural Science Foundation of China [31430003]
Poly(3-hydroxypropionate) (P3 HP) is the strongest family member of microbial polyhydroxyalkanoates (PHA) synthesized by bacteria grown on 1,3-propandiol or glycerol. In this study synthesis pathways of P3HP and its copolymer P3HB3HP of 3-hydroxybutyrate (3HB) and 3-hydroxypropionate (3HP) were assembled respectively to allow their synthesis from glucose, a more abundant carbon source. Recombinant Escherichia cob was constructed harboring the P3HP synthetic pathway consisting of heterologous genes encoding glycerol-3-phosphate dehydrogenase (gpd1), glycerol-3-P phosphatase (gpp2) from Sac charomyces cerevisiue that catalyzes formation of glycerol from glucose, and genes coding glycerol dehydratase (dhaB123) with its reactivating factors (gdrAB) from Klebsiellu pneumoniae that transfer glycerol to 3-hydroxypropionaldehyde, as well as gene encoding propionaldehyde dehydrogenase (pdup) from Salmonella typhimurium which converts 3-hydroxypropionaldehyde to 3-hydroxypropionyl-CoA, together with the gene of PHA synthase (phaC) from Ralstonia eutropha which polymerizes 3-hydroxypropionyl-CoA into P3HP. When phaA and phaB from Ralstonia eutropha respectively encoding 1-3-ketothiolase and acetoacetate reductase, were introduced into the above P3HP producing recombinant E. coli, copolymers poly(3-hydroxybutyrate-co-3-hydroxypropionate) (P3HB3HP) were synthesized from glucose as a sole carbon source. The above E. coil recombinants grown on glucose LB medium successfully produced 5 g/L cell clry weight containing 18% P3HP and 42% P(3HB-co-84 mol% 3HP), respectively, in 48 h shake flask studies. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
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