期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 110, 期 8, 页码 989-995出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/0074-02760150222
关键词
leptospirosis; Leptospira; LigBrep; subunit vaccine; DNA vaccine; prime-boost
资金
- CAPES
- CNPq
- FAPERGS
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain of Leptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.
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