Article
Oncology
Chunyang Chen, He Wang, Xinyu Geng, Dongze Zhang, Zhengyu Zhu, Guangbo Zhang, Jianquan Hou
Summary: This study confirmed the inhibitory effect of metformin on prostate cancer cell lines, with mechanisms involving autophagy and cell cycle arrest at G0/G1 phase. Metformin induced AMPK activation, promoted LC3II expression, and down-regulated p62/SQSTM1 expression. Animal experiments demonstrated that metformin reduced tumor volume, upregulated p-AMPK (Thr172) and LC3B, and downregulated Ki-67 levels without abnormal biochemical indicators.
CANCER CELL INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Shisong Fang, Lin Zhang, Yingzhi Liu, Wenye Xu, Weihua Wu, Ziheng Huang, Xin Wang, Hui Liu, Ying Sun, Renli Zhang, Bo Peng, Xiaodong Liu, Xiao Sun, Jun Yu, Francis Ka Leung Chan, Siew Chien Ng, Sunny Hei Wong, Maggie Hai Tian Wang, Tony Gin, Gavin Matthew Joynt, David Shu Cheong Hui, Tiejian Feng, William Ka Kei Wu, Matthew Tak Vai Chan, Xuan Zou, Junjie Xia
Summary: The study found that lysosome function is dysregulated in PBMCs isolated from COVID-19 patients, and the biomarker LC3B can be used to differentiate between patients with moderate-to-severe disease and those with asymptomatic or mild disease. Patients with an LC3B concentration<5.5 ng/ml will require early hospital admission for oxygen therapy.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Young-Eun Cho, Yong-Jun Kim, Sun Lee, Jae-Hoon Park
Summary: In this study, a novel nucleolar-cytoplasmic axis involving nucleolar protein NOP53 was identified to regulate the cytoplasmic autophagy process through ZKSCAN3-dependent and -independent pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Yuko Shirono, Vladimir Bilim, Tsutomu Anraku, Hiroo Kuroki, Akira Kazama, Masaki Murata, Kaede Hiruma, Yoshihiko Tomita
Summary: In advanced bladder cancer, targeting GSK-3 beta and autophagy-related proteins can reduce tumor cell proliferation and induce apoptosis.
Article
Cell Biology
Elif Beguem Goekerkuecuek, Angelique Cheron, Marc Tramier, Giulia Bertolin
Summary: Although the mechanisms of autophagy have been studied, real-time monitoring of this process is still difficult. Researchers developed a biosensor using FRET to observe the priming of the autophagy player LC3B by ATG4B. This biosensor allows for spatial characterization of the priming activity and quantification of autophagy activation. The study also revealed the regulation of the ATG4B-LC3B axis during mitosis.
Article
Cell Biology
Mengmeng Zhao, Fei Wang, Juehui Wu, Yuanna Cheng, Yajuan Cao, Xiangyang Wu, Mingtong Ma, Fen Tang, Zhi Liu, Haipeng Liu, Baoxue Ge
Summary: The study shows that CGAS can reduce the abundance of micronuclei by promoting autophagy, thus maintaining micronuclei homeostasis. The direct interaction between CGAS and MAP1LC3B is crucial for the autophagic clearance of micronuclei, leading to inhibition of cGAMP production induced by genotoxic stress. This research reveals an important role of CGAS in the selective autophagic clearance of micronuclei.
Article
Cell Biology
Mengmeng Chen, Yushan Fu, Xu Wang, Ruitong Wu, Dongmei Su, Nan Zhou, Yanhua Qi
Summary: The senescence of lens epithelial cells (LECs) is a major factor leading to age-related cataract (ARC). This study aimed to investigate the mechanism of ARC formation and explore the anti-aging effect of metformin (MET) on ARC. The results showed that MET effectively alleviated the senescence of LECs and the formation of ARC probably through inactivation of the AMPK pathway and augmentation of autophagy.
CELL DEATH DISCOVERY
(2022)
Article
Pharmacology & Pharmacy
Minmin Fan, Jian Gao, Lin Zhou, Wenwen Xue, Yixuan Wang, Jingwei Chen, Wuhao Li, Ying Yu, Bo Liu, Yan Shen, Qiang Xu
Summary: The expression of SERCA2 in triple negative breast cancer (TNBC) patients is closely related to disease progression, and it also promotes the growth, migration and chemoresistance of breast cancer cells. Through experiments, it is proven that SERCA2 can interact with LC3B, induce cell autophagy, and increase the tolerance of breast cancer cells to drugs. In addition, it is found that targeting SERCA2 can increase cell autophagy and induce cell death, achieving the therapeutic effect of treating breast cancer.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Biochemistry & Molecular Biology
Liudmila Spirina, Alexandra Avgustinovich, Olga Bakina, Sergey G. Afanas'ev, Maxim Yu Volkov, Amina Y. Kebekbayeva
Summary: Autophagy plays a dual role in oncogenesis processes, enhancing cell resistance to oncogenic factors while also participating in tumor progression. This study found associations between the effectiveness of the FLOT regimen in resectable gastric cancers and the key autophagy-related proteins LC3B, mTOR, and AMPK. These proteins were identified as molecular targets affecting cancer progression, metastasis, and the effectiveness of neoadjuvant chemotherapy.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Jiahua Zheng, Shaochai Wei, Tingting Xiao, Guanwu Li
Summary: This study demonstrates that resveratrol induces non-canonical autophagy and apoptosis activation simultaneously in A549 lung cancer cells, while mitophagy mediated by LC3B/p62 interaction protects tumor cells against apoptosis.
Article
Cardiac & Cardiovascular Systems
Cevher Ozcan, Gunjan Dixit, Zhenping Li
Summary: The study showed that pharmacological activation of AMPK can effectively prevent the occurrence of atrial fibrillation in a specific mouse model by improving mitochondrial function and reducing fibrosis in the atria.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2021)
Article
Cell Biology
Julien Bensalem, Kathryn J. Hattersley, Leanne K. Hein, Xiao Tong Teong, Julian M. Carosi, Sofia Hassiotis, Randall H. Grose, Celia Fourrier, Leonie K. Heilbronn, Timothy J. Sargeant
Summary: Autophagic flux, a critical cellular process, can be increased by targeting nutrition-related biochemical signaling. This study introduces a method to directly measure human autophagic flux from blood samples by treating whole blood and using isolated cells to measure LC3B-II protein. The method is valuable for evaluating intra-individual variation induced by interventions.
Article
Biochemistry & Molecular Biology
Khudhair Rashid Khudhair Alzubaidi, Majid Mahdavi, Sanam Dolati, Mehdi Yousefi
Summary: This study found that autophagy is elevated in women with preeclampsia compared to healthy pregnant women, contradicting some previous studies. Further research is needed to explore the role of autophagy in pre-eclamptic women.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Medicine, General & Internal
Gregory F. Michaud, William G. Stevenson
Summary: A 63-year-old healthy male with atrial fibrillation presents with symptoms of dyspnea and irregular heartbeat. Physical examination reveals high blood pressure, irregular pulse, and ECG confirms atrial fibrillation. Further evaluation and treatment plan are needed.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Cell Biology
Sriganesh Ramachandra Rao, Steven J. Fliesler
Summary: The tandem-tagged autophagy reporter mouse model is a useful tool for studying autophagy in the retina, but its application depends on various technical and biological factors, including microscope settings, transgene expression, choice of fluorophores, and the presence of lipofuscin autofluorescence.
