Article
Cell & Tissue Engineering
Kai Kretzschmar, Kim E. Boonekamp, Margit Bleijs, Priyanca Asra, Mandy Koomen, Susana M. Chuva de Sousa Lopes, Barbara Giovannone, Hans Clevers
Summary: Research has identified Troy as a marker of epidermal basal cells that play a role in the long-term renewal and cornification of the skin. Single-cell transcriptomics and organoid assays confirmed their stem cell identity and ability to generate differentiating daughter cells.
Article
Oncology
Minhong Shen, Shanshan Xie, Michelle Rowicki, Sven Michel, Yong Wei, Xiang Hang, Liling Wan, Xin Lu, Min Yuan, John F. Jin, Frank Jaschinski, Tianhua Zhou, Richard Klar, Yibin Kang
Summary: The study found that MTDH promotes the progression of colorectal and lung cancers by facilitating Wnt activation and inducing T-cell exhaustion. Treatment with MTDH antisense oligonucleotides effectively suppresses MTDH expression and significantly attenuates the progression and metastasis of these cancers.
Article
Biochemistry & Molecular Biology
Maria Abancens, Brian J. Harvey, Jean McBryan
Summary: Women have lower incidence and mortality rates for colorectal cancer compared to men. Estrogen may protect against colorectal cancer progression through GPER-mediated modulation of the Wnt/beta-catenin pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Heyu Song, Ricky A. Sontz, Matthew J. Vance, Julia M. Morris, Sulaiman Sheriff, Songli Zhu, Suzann Duan, Jiping Zeng, Erika Koeppe, Ritu Pandey, Curtis A. Thorne, Elena M. Stoffel, Juanita L. Merchant
Summary: The incidence of early-onset colorectal cancer (EO-CRC) is rising, with lifestyle factors and genetic background playing a potential role. The study found a missense mutation, HNF1AA98V, in the Hepatic Nuclear Factor 1α (HNF1A) gene, which reduces DNA binding ability. In mice, introducing the HNF1AA98V variant and feeding a high-fat or high-sugar diet led to an increased formation of colonic polyps. The study also revealed alterations in gene expression and signaling pathways related to metabolism, immunity, and lipid biogenesis.
Article
Biochemistry & Molecular Biology
Chuan-Jian Shi, Shi-Ying Li, Chun-Hui Shen, Fei-Fei Pan, Li-Qiang Deng, Wei-Ming Fu, Ji-Yong Wang, Jin-Fang Zhang
Summary: In this study, it was found that Icariside II suppressed the proliferation of colorectal cancer (CRC) by inducing cell cycle arrest and apoptosis. It also inhibited tumor growth in vivo by suppressing the expression of beta-catenin and inactivating the Wnt/beta-catenin signaling pathway. Furthermore, Icariside II was shown to epigenetically target DNA methyltransferases (DNMTs) and decrease global DNA methylation levels, resulting in the suppression of circ beta-catenin biogenesis.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Junrong Liang, Lina Sun, Yujun Li, Wanning Liu, Danxiu Li, Ping Chen, Xin Wang, Juan Hui, Jinchi Zhou, Hao Liu, Tianyu Cao, Maogui Pang, Meng Guo, Xin Wang, Xiaodi Zhao, Yuanyuan Lu
Summary: This study investigated the expression and function of DKK4 in colorectal cancer (CRC). The results showed that DKK4 was upregulated in CRC cell lines and tissues, and it inhibited cell metastasis by repressing the Wnt signaling pathway. Through experiments and analysis, the researchers also revealed the regulatory mechanism of DKK4 and its potential therapeutic application.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Vikash Singh, Vonn Walter, Irina Elcheva, Yuka Imamura Kawasawa, Vladimir S. Spiegelman
Summary: In this study, the role of IGF2BP1 in the post-transcriptional regulation of Wnt/beta-catenin-induced genes in colorectal cancer (CRC) cells was investigated using iCLIP and RNA sequencing techniques. The study revealed that CRC cells exhibit a stronger dependency on IGF2BP1 expression for Wnt/beta-catenin-regulated genes compared to normal cells. iCLIP analysis showed significant changes in IGF2BP1-binding sites and enrichment of 6-mer motifs associated with IGF2BP1 binding in response to Wnt/beta-catenin signaling. Additionally, a signature of IGF2BP1-regulated genes significantly associated with colon cancer-free survival in humans was identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Pu-Hyeon Cha, Jeong-Ha Hwang, Dong-Kyu Kwak, Eunjin Koh, Kyung-Sup Kim, Kang-Yell Choi
Summary: In colorectal cancer, the loss of APC activates Wnt/beta-catenin signaling, leading to the Warburg effect and affecting cancer cell metabolism. PKM2 and LDHA were identified as key molecules regulated by Wnt/beta-catenin signaling, with APC loss increasing their expression, resulting in increased glucose consumption and lactate secretion.
BRITISH JOURNAL OF CANCER
(2021)
Article
Pharmacology & Pharmacy
Young Yun Jung, Jae-Young Um, Arunachalam Chinnathambi, Chandramohan Govindasamy, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Gautam Sethi, Kwang Seok Ahn
Summary: This study investigated the effects of Withaferin A (WFA) on apoptosis and autophagy in human colorectal cancer cells. The results showed that WFA could induce cell cycle arrest, increase apoptotic cell death, and activate autophagy. The WFA-induced effects on apoptosis and autophagy were mediated through the modulation of the beta-catenin pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Oncology
Jinlai Wei, Xiangru Zheng, Wenjun Li, Xiaoli Li, Zhongxue Fu
Summary: This study found that Sestrin2 inhibits the progression of colorectal cancer by suppressing cell proliferation, migration, and colony formation. It also affects the cancer stemness properties. This is achieved through downregulation of the Wnt signaling pathway. The findings suggest that Sestrin2 could be a promising therapeutic target for colorectal cancer.
CANCER CELL INTERNATIONAL
(2022)
Article
Oncology
Lanqing Wang, Lei Zhao, Zhenyu Lin, Dandan Yu, Min Jin, Pengfei Zhou, Jinghua Ren, Jing Cheng, Kunyu Yang, Gang Wu, Tao Zhang, Dejun Zhang
Summary: This study reveals that DCLK1 promotes 5-fluorouracil resistance in colorectal cancer through the CCAR1/beta-catenin pathway-mediated cancer stemness. The results suggest that targeting DCLK1 might be a promising approach to overcome 5-fluorouracil resistance in colorectal cancer by eliminating cancer stem cells.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Medicine, Research & Experimental
Yong Ji, Jian Lv, Di Sun, Yufeng Huang
Summary: This review explores the significant role of the Wnt/β-catenin signaling pathway in colorectal cancer tumorigenesis, metastasis, and recurrence, as well as its potential value in diagnosis and therapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yuyuan Zi, Liyu Liu, Jie Gao, Xu Xu, Yidi Guan, Zhuoxian Rong, Zhen Cao, Mengwei Li, Zimei Zeng, Qi Fan, Feiyu Tang, Junju He, Dan Feng, Jionghuang Chen, Yuedi Dai, Yufeng Huang, Yingjie Nie, Haiping Pei, Qingping Cai, Zhi Li, Lunquan Sun, Yuezhen Deng
Summary: This study finds that pancreatic progenitor cell differentiation and proliferation factor (PPDPF) is upregulated in colorectal cancer (CRC) and is significantly correlated with TNM stages and survival time. Knockout of PPDPF in the intestinal epithelium shortens crypts, decreases the number of stem cells, and inhibits CRC occurrence. Mechanistically, PPDPF interacts with CK1 alpha, disrupting its binding to Axin and activating the Wnt/beta-catenin pathway. Inflammatory signals mediated by IL6/JAK2 phosphorylate PPDPF, stabilizing the protein. Overall, PPDPF is a key molecule connecting inflammation to the Wnt/beta-catenin signaling pathway in CRC carcinogenesis and progression, suggesting it as a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Yung-Fu Wu, Chih-Yang Wang, Wan-Chun Tang, Yu-Cheng Lee, Hoang Dang Khoa Ta, Li-Chia Lin, Syu-Ruei Pan, Yi-Chun Ni, Gangga Anuraga, Kuen-Haur Lee
Summary: Colorectal cancer is a heterogeneous disease with genetic and epigenetic changes, and molecular assessment along with biomarker use for prognosis is gaining attention. This study identifies high mRNA levels of 10 upregulated genes in CRC cells and datasets, showing correlation with worse prognosis in CRC patients. CTNNB1 is found to be a key factor in the interaction with upregulated CRC-associated genes, particularly regulating NKD2, FOXQ1, and CEMIP, which when inhibited, can suppress CRC cell growth, providing efficient biomarkers for prognosis and a new molecular interaction network for CRC.
Article
Oncology
Dafeng Tong, Jie Yuan, Zhaoming Wang, Enda Yu, E. Jifu
Summary: Ubiquitin-specific peptidase 37 (USP37) is up-regulated in colorectal cancer (CRC) and its high expression is associated with poor patient survival. Up-regulation of USP37 promotes various malignant characteristics of CRC cells, including proliferation, migration, invasion, and stemness, and also enhances angiogenesis. Silencing USP37 shows the opposite effects. Mechanistically, USP37 stabilizes beta-catenin by inhibiting its ubiquitination. CTNNB1, the gene encoding beta-catenin, is positively correlated with USP37 in CRC. Targeting USP37 may hold potential for CRC treatment.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)