Article
Multidisciplinary Sciences
Guyu Qin, Eun-Sil Park, Xueqing Chen, Sen Han, Dongxi Xiang, Fang Ren, Gang Liu, Huidong Chen, Guo-Cheng Yuan, Zhe Li
Summary: Epithelial ovarian cancer (EOC) can originate from either fallopian tube epithelial (FTE) or ovarian surface epithelial (OSE) cells, but they exhibit different latencies and disease outcomes. Single cell RNA-sequencing revealed that FTE secretory stem/progenitor cells resemble mammary luminal progenitors, while OSE cells resemble basal cells. Additionally, an FT stromal subpopulation may serve as a niche for FTE cells, while Lgr5(+) OSE cells may self-sustain as their own niche. The differences in intrinsic expression programs and niche organizations of FTE and OSE cells may contribute to the development of EOCs.
Article
Biochemistry & Molecular Biology
Maobi Zhu, Na Wang, Sha Wang, Yao Wang, Xiawen Yang, Jianglin Fan, Yajie Chen
Summary: This study examined the injury and repair mechanism of the fallopian tube, the original site of high-grade serous ovarian carcinoma. The results showed that follicular fluid stimulation promotes cell differentiation and organoid formation, but also causes cell injury and apoptosis at high concentrations. These findings contribute to the investigation of the initiation mechanisms of high-grade serous ovarian carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Pawel Uruski, Agnieszka Sepetowska, Corinna Konieczna, Martyna Pakula, Michal Wyrwa, Akylbek Tussupkaliyev, Andrzej Tykarski, Justyna Mikula-Pietrasik, Krzysztof Ksiazek
Summary: The study found that the combination of carboplatin and paclitaxel can induce senescence in ovarian cancer cells in vitro, leading to features such as cell growth arrest, increased expression of senescence biomarkers, upregulated expression of cell cycle inhibitors, and increased mitochondrial mass. Senescent cells did not show changes in telomere length or telomerase activity, and most DNA damage was localized outside telomeres.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Angelica Giuliani, Anna Maria Giudetti, Daniele Vergara, Laura Del Coco, Deborah Ramini, Sara Caccese, Matilde Sbriscia, Laura Graciotti, Gianluca Fulgenzi, Luca Tiano, Francesco Paolo Fanizzi, Fabiola Olivieri, Maria Rita Rippo, Jacopo Sabbatinelli
Summary: Cellular senescence is closely linked to endothelial dysfunction, resulting in chronic inflammation and vascular impairments. The metabolic behavior of senescent endothelial cells is complex and plays a role in the proinflammatory phenotype.
Article
Cell Biology
Pamoda Galhenage, Yunlan Zhou, Erica Perry, Brenda Loc, Kelly Fietz, Sonia Iyer, Ferenc Reinhardt, Tiego Da Silva, Vladimir Botchkarev, Jie Chen, Christopher P. Crum, Robert A. Weinberg, Shailja Pathania
Summary: Clinical and molecular evidence suggests that high-grade serous ovarian cancer primarily originates from the fallopian tube due to distinct differences between fallopian tube epithelial cells and ovarian surface epithelial cells. Fallopian tube cells exhibit heightened replication stress, impaired repair mechanisms, defective checkpoint functionality, and increased tumorigenicity compared to ovarian surface cells. BRCA1 heterozygosity exacerbates these defects and leads to an aggressive tumor phenotype.
Article
Cell Biology
Daniel Bronder, Anthony Tighe, Darawalee Wangsa, Dali Zong, Thomas J. Meyer, Rene Wardenaar, Paul Minshall, Daniela Hirsch, Kerstin Heselmeyer-Haddad, Louisa Nelson, Diana Spierings, Joanne C. McGrail, Maggie Cam, Andre Nussenzweig, Floris Foijer, Thomas Ried, Stephen S. Taylor
Summary: The study investigated the pathogenesis of HGSOC and found that TP53 mutation alone is sufficient to induce subclonal karyotype alterations, which are exacerbated by the loss of BRCA1, leading to transcriptional deregulation and karyotype diversity, ultimately causing the deregulation of cell cycle control networks and the initiation of CIN.
DISEASE MODELS & MECHANISMS
(2021)
Article
Oncology
Jingni Wu, Yael Raz, Maria Sol Recouvreux, Marcio Augusto Diniz, Jenny Lester, Beth Y. Karlan, Ann E. Walts, Arkadiusz Gertych, Sandra Orsulic
Summary: This study aimed to investigate the global visual and subvisual microenvironmental differences between fallopian tubes with and without STIC lesions. The results showed differences in stromal and epithelial cells, and also a correlation between age and cell type changes.
FRONTIERS IN ONCOLOGY
(2022)
Article
Reproductive Biology
Szymon Rutecki, Paulina Szulc, Martyna Pakula, Pawel Uruski, Artur Radziemski, Eryk Naumowicz, Rafal Moszynski, Andrzej Tykarski, Justyna Mikula-Pietrasik, Krzysztof Ksiazek
Summary: Spontaneous senescence of primary epithelial ovarian cancer cells (pEOCs) should be considered as an independent pathogenetic factor in cancer progression. These cancer cells promote cancer growth and spread by releasing pro-cancerous proteins and mediators that affect surrounding tissues and vascular endothelium. Spontaneously senescent ovarian cancer cells are more efficient in promoting cancer development compared to drug-induced senescent cells.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Christine Hansel, Samantha Barr, Alina Schemann, Kirsten Lauber, Julia Hess, Kristian Unger, Horst Zitzelsberger, Verena Jendrossek, Diana Klein
Summary: Radiation-induced damage to normal lung tissue can be mitigated by inhibiting cellular senescence and SASP expression in lung epithelial cells, thereby reducing vascular dysfunction and extravasation of immune and tumor cells. Metformin treatment shows potential in protecting normal lung tissue from radiation-induced senescence and associated lung injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sweta Dash, Sean Yoder, Tania Mesa, Andrew Smith, Ling Cen, Steven Eschrich, Guillermo N. Armaiz-Pena, Alvaro N. A. Monteiro
Summary: The study found that sustained adrenergic stimulation by norepinephrine has significant effects on ovarian cancer initiation and progression, particularly affecting ovarian surface epithelial cells and fallopian tube cells differently. Mutations in the p53 gene also influence the cellular response to NE stimulation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Tova M. Bergsten, Sarah E. Levy, Katherine E. Zink, Hannah J. Lusk, Melissa R. Pergande, Stephanie M. Cologna, Joanna E. Burdette, Laura M. Sanchez
Summary: This study discovered that a secreted protein called SPARC, produced by tumorigenic fallopian tube epithelial cells, enhances the release of norepinephrine (NE) from the ovary, influencing the primary metastasis of high grade serous ovarian cancer (HGSOC).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maobi Zhu, Sen Takeda, Tomohiko Iwano
Summary: The study found that phytoestrogens can induce the generation of multi-ciliated cells, improving the balance of fallopian tube epithelial sheet, and this effect depends on the ERβ-mediated pathway.
Article
Immunology
Yuki Nakamura, Riho Aihara, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
Summary: The study found that IL1A or IL1B can induce SASP production in bovine OECs, but neither of them cause cellular senescence or migration of polymorphonuclear neutrophils. IL1B treatment of OEC-cultured medium significantly induced PMN migration, and CCL2 induced by IL1B is essential for immune cell migration to OECs.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Oncology
Hyeong In Ha, Eun Young Park, Kyung Jin Eoh, Yeon Jee Lee, Sang-Soo Seo, Sokbom Kang, Sang-Yoon Park, Myong Cheol Lim
Summary: This study investigated the clinical significance of the ovarian cancer cluster region (OCCR) in BRCA1 and BRCA2 genes. The results showed that patients with BRCA1 pathogenic variants (PVs) in the OCCR region had shorter progression-free survival (PFS) compared to those outside the OCCR. This trend was more pronounced in the platinum-sensitive subgroup. However, no significant differences were found in overall survival (OS) between patients with BRCA1/2 PVs, regardless of the variant location.
GYNECOLOGIC ONCOLOGY
(2022)
Article
Oncology
Jose A. Colina, Katherine E. Zink, Kanella Eliadis, Reza Salehi, Emma S. Gargus, Sarah R. Wagner, Kristine J. Moss, Seth Baligod, Kailiang Li, Brenna J. Kirkpatrick, Teresa K. Woodruff, Benjamin K. Tsang, Laura M. Sanchez, Joanna E. Burdette
Summary: The chemical communication between the fallopian tube and the ovary plays a crucial role in the development of high grade serous ovarian cancer, with the fimbriated ends being the progenitor site of HGSOC. Androgen exposure may promote ovarian carcinogenesis by increasing proliferation and preneoplastic lesions in the fallopian tube.
Article
Medical Laboratory Technology
Vincent H. J. van der Velden, Frank Preijers, Ulrika Johansson, Theresia M. Westers, Alan Dunlop, Anna Porwit, Marie C. Bene, Peter Valent, Jeroen te Marvelde, Orianne Wagner-Ballon, Uta Oelschlaegel, Leonie Saft, Sharham Kordasti, Robin Ireland, Eline Cremers, Canan Alhan, Carolien Duetz, Willemijn Hobo, Nicolas Chapuis, Michaela Fontenay, Peter Bettelheim, Lisa Eidenshink-Brodersen, Patricia Font, Michael R. Loken, Sergio Matarraz, Kiyoyuki Ogata, Alberto Orfao, Katherina Psarra, Dolores Subira, Denise A. Wells, Matteo G. Della Porta, Kate Burbury, Frauke Bellos, Elisabeth Weiss, Wolfgang Kern, Arjan van de Loosdrecht
Summary: This article reviews the progress of flow cytometry (FCM) in the diagnosis of myelodysplastic syndrome (MDS) in recent years, and provides optimal methods and recommendations for sample processing, antibody panels and fluorochromes, and current hardware technologies. These recommendations will facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients, but further standardization and harmonization are needed.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Medical Laboratory Technology
Theresia M. Westers, Leonie Saft, Vincent H. J. van der Velden, Jeroen G. te Marvelde, Alan Dunlop, Robin Ireland, Peter Valent, Anna Porwit, Marie C. Bene, Arjan A. van de Loosdrecht
Summary: This study described the diagnostic workflow of six cases using multimodal integrated diagnostics and demonstrated the application and added value of flow cytometry in patients with myelodysplastic syndrome.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Medical Laboratory Technology
Arjan A. Loosdrecht, Wolfgang Kern, Anna Porwit, Peter Valent, Sharham Kordasti, Eline Cremers, Canan Alhan, Carolien Duetz, Alan Dunlop, Willemijn Hobo, Frank Preijers, Orianne Wagner-Ballon, Nicolas Chapuis, Michaela Fontenay, Peter Bettelheim, Lisa Eidenschink-Brodersen, Patricia Font, Ulrika Johansson, Michael R. Loken, Jeroen G. Marvelde, Sergio Matarraz, Kiyoyuki Ogata, Uta Oelschlaegel, Alberto Orfao, Katherina Psarra, Dolores Subira, Denise A. Wells, Marie C. Bene, Matteo G. Della Porta, Kate Burbury, Frauke Bellos, Vincent H. J. van der Velden, Theresia M. Westers, Leonie Saft, Robin Ireland
Summary: This article discusses the rationale for including flow cytometry (FCM) in the diagnostic investigation and evaluation of cytopenias and suspected myelodysplastic syndromes (MDS) by the European LeukemiaNet international MDS Flow Working Group. The WHO 2016 classification acknowledges the contribution of FCM in the diagnosis of MDS and its potential usefulness in prognostication, prediction, therapy evaluation, and patient follow-up.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Allergy
Dubravka Smiljkovic, Harald Herrmann, Irina Sadovnik, Susanne Gamperl, Daniela Berger, Gabriele Stefanzl, Gregor Eisenwort, Gregor Hoermann, Sonja Kopanja, Yulia Dorofeeva, Margarete Focke-Tejkl, Peter Jaksch, Konrad Hoetzenecker, Zsolt Szepfalusi, Rudolf Valenta, Michel Arock, Peter Valent
Summary: Siglec-6 is identified as a surface antigen specifically expressed on human MC and basophils. Its expression is dynamically regulated and varies depending on cell types and activation factors. Understanding the expression changes of Siglec-6 provides insights into the function of MC and basophils in allergic reactions.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Review
Allergy
Peter Valent, Amy D. Klion, Florence Roufosse, Dagmar Simon, Georgia Metzgeroth, Kristin M. Leiferman, Juliana Schwaab, Joseph H. Butterfield, Wolfgang R. Sperr, Karl Sotlar, Peter Vandenberghe, Gregor Hoermann, Torsten Haferlach, Richard Moriggl, Tracy George, Cem Akin, Bruce S. Bochner, Jason Gotlib, Andreas Reiter, Hans-Peter Horny, Michel Arock, Hans-Uwe Simon, Gerald J. Gleich
Summary: Eosinophilia and eosinophil activation are common in various diseases. They can lead to hypereosinophilia and hypereosinophilic syndrome, which can cause organ damage. The classification and criteria for these conditions have been widely accepted and regarded as standard since 2012. However, new developments in the field have created a need to update these criteria. A recent working conference on eosinophil disorders discussed new markers and concepts, aiming to improve the diagnosis and management of patients with hypereosinophilia and hypereosinophilic syndrome.
Article
Biochemistry & Molecular Biology
Anna Fosch, Maria Rodriguez-Garcia, Cristina Miralpeix, Sebastian Zagmutt, Maite Larranaga, Ana Cristina Reguera, Jesus Garcia-Chica, Laura Herrero, Dolors Serra, Nuria Casals, Rosalia Rodriguez-Rodriguez
Summary: Sensing of long-chain fatty acids (LCFA) in the hypothalamus modulates energy balance and disruption leads to obesity. The effects of saturated or unsaturated LCFA on hypothalamic-brown adipose tissue (BAT) axis and underlying mechanisms were investigated. Unsaturated LCFA increased BAT thermogenesis through CPT1C-mediated FA metabolism shift, while saturated LCFA did not. These findings provide new insight into neuronal circuitries activated by LCFA to drive thermogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Karin Schelch, Sebastian Eder, Benjamin Zitta, Monica Phimmachanh, Thomas G. Johnson, Dominik Emminger, Andrea Wenninger-Weinzierl, Caterina Sturtzel, Hugo Poplimont, Alexander Ries, Konrad Hoetzenecker, Mir A. Hoda, Walter Berger, Martin Distel, Balazs Dome, Glen Reid, Michael Grusch
Summary: Pleural mesothelioma (PM) is a highly aggressive cancer with limited treatment options. The oncoprotein Y-box-binding protein-1 (YB-1) is overexpressed in cancer and has been shown to promote cancer cell migration. This study investigates the role of YB-1 in PM cell behavior and identifies snail as a critical regulator of YB-1-mediated cell motility. The findings provide insights into the mechanism underlying PM's aggressive nature and highlight the importance of YB-1 in this cancer.
MOLECULAR ONCOLOGY
(2023)
Review
Cell Biology
Rut Fado, Sebastian Zagmutt, Laura Herrero, Helena Muley, Rosalia Rodriguez-Rodriguez, Huichang Bi, Dolors Serra, Nuria Casals
Summary: There is a need for reliable genetic biomarkers in the diagnosis, prognosis, and treatment of different tumor types. One potential biomarker is CPT1C, a neuronal protein involved in cancer cell adaptation to nutrient depletion and hypoxia. However, its molecular role in cancer cells is controversial. This review discusses the evidence on CPT1C in neurons and its potential role in lipid metabolism and cell adaptation to stressors in cancer cells.
CELL DEATH & DISEASE
(2023)
Article
Pharmacology & Pharmacy
Theresa Mendrina, Isabella Poetsch, Hemma Schueffl, Dina Baier, Christine Pirker, Alexander Ries, Bernhard K. K. Keppler, Christian R. R. Kowol, Dan Gibson, Michael Grusch, Walter Berger, Petra Heffeter
Summary: Platinum compounds are effective treatment options for various cancer types, but their use is limited by side effects and drug resistance. A new multi-targeted platinum(IV) prodrug, cisPt(PhB)(2), has shown promising anticancer activity and the ability to overcome drug resistance mechanisms. The drug's mode of action is influenced by cellular fatty acid metabolism and mitochondrial activity. Specifically, it is more effective against cisplatin-resistant cancer cells with Warburg-like properties. This orally applicable prodrug has the potential to improve cancer treatment outcomes.
Article
Medicine, General & Internal
Ramon Colomer, Jesus Miranda, Nuria Romero-Laorden, Javier Hornedo, Lucia Gonzalez-Cortijo, Silvana Mouron, Maria J. Bueno, Rebeca Mondejar, Miguel Quintela-Fandino
Summary: NGS testing does not significantly influence the clinical course, but can improve progression-free survival when performed within clinical judgement.
Review
Endocrinology & Metabolism
Ivonne Palacios-Marin, Dolors Serra, Josep. C. C. Jimenez-Chillaron, Laura Herrero, Marijana Todorcevic
Summary: Obesity is a major risk factor for type 2 diabetes and cardiovascular diseases. Childhood obesity is a concerning health challenge as it often persists into adulthood and increases the risk of morbidity and mortality. The mechanisms through which adipose tissue expands and its implications on metabolic health are still unclear, and more research is urgently needed to understand the development of adipose tissue in healthy and obese children.
Review
Nutrition & Dietetics
Ivonne Palacios-Marin, Dolors Serra, Josep Jimenez-Chillaron, Laura Herrero, Marijana Todorcevic
Summary: The increase in obesity worldwide has led to metabolic health complications, and understanding the cellular and lipid turnover in adipose tissue is crucial for mitigating the adverse effects of excess adiposity.
Article
Cell Biology
Alexander Ries, Astrid Slany, Christine Pirker, Johanna C. C. Mader, Doris Mejri, Thomas Mohr, Karin Schelch, Daniela Flehberger, Nadine Maach, Muhammad Hashim, Mir Alireza Hoda, Balazs Dome, Georg Krupitza, Walter Berger, Christopher Gerner, Klaus Holzmann, Michael Grusch
Summary: In this study, novel hTERT-transduced mesothelial cell and Meso-CAF models were generated and characterized, and their impact on PM cell growth was investigated.
Article
Oncology
Rebeca Jimeno, Silvana Mouron, Roberto Salgado, Sherene Loi, Belen Perez-Mies, Rodrigo Sanchez-Bayona, Luis Manso, Mario Martinez, Ana Garrido-Garcia, Rosario Serrano-Pardo, Ramon Colomer, Miguel Quintela-Fandino
Summary: This study evaluated the immune microenvironment in TNBC tumors and found that the infiltration of TILs and the profiling of lymphocytes varied in tumors with hyperactivation of P70S6K. Decreased CD20(+) cells and CD8(+)/CD4(+) ratio were observed in high p-P70S6K tumors. The p-P70S6K score was positively correlated with CD4(+) and Foxp3(+) T cells, while it was negatively correlated with CD8(+)/CD4(+) and CD8(+)/Foxp3(+) ratios.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Oncology
Maria Jose Bueno, Silvana Mouron, Eduardo Caleiras, Mario Martinez, Luis Manso, Ramon Colomer, Miguel Quintela-Fandino
Summary: By evaluating the expression levels of HER2, TROP2, and PD-L1 in TNBC patients, we found that early HER2-low, PD-L1-positive patients have a favorable prognosis, particularly when treated with anthracycline/taxane- or carboplatin-containing regimes.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)