Article
Oncology
Gabrielle Leclercq, Helene Haegel, Anneliese Schneider, Anna Maria Giusti, Estelle Marrer-Berger, Christophe Boetsch, Antje-Christine Walz, Vesna Pulko, Johannes Sam, John Challier, Cristiano Ferlini, Alex Odermatt, Pablo Umana, Marina Bacac, Christian Klein
Summary: The study demonstrates that dasatinib can effectively serve as a pharmacological on/off switch to mitigate off-tumor toxicities or CRS induced by T cell bispecific antibodies. By providing pharmacologically relevant doses, dasatinib can regulate T cell activation, cell killing, and cytokine release triggered by T cell engagers.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Hongling Huang, Peipei Zhou, Jun Wei, Lingyun Long, Hao Shi, Yogesh Dhungana, Nicole M. Chapman, Guotong Fu, Jordy Saravia, Jana L. Raynor, Shaofeng Liu, Gustavo Palacios, Yong-Dong Wang, Chenxi Qian, Jiyang Yu, Hongbo Chi
Summary: This study reveals that amino acid transporters play a role in dampening T-MEM differentiation, while Pofut1 is a key regulator for terminal effector differentiation of T-EFF cells. These findings highlight the importance of nutrient uptake and signaling in determining T cell fate.
Article
Biochemistry & Molecular Biology
Silvia Carloni, Claudia Piccinini, Elena Pancisi, Valentina Soldati, Monica Stefanelli, Anna Maria Granato, Toni Ibrahim, Massimiliano Petrini
Summary: A novel bioassay utilizing flow cytometry as a read-out measurement was developed and validated for evaluating the potency of DC-based cellular therapies. The method was found to be specific, accurate, and compliant with international guidelines, showing high accuracy and reproducibility in measuring DC potency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rania M. Kamal, Manal M. Sabry, Zeinab Y. Aly, Mohamed S. Hifnawy
Summary: This study evaluated the in vivo anti-arthritic potential of a specific Aloe species' leaf ethanolic extract, both alone and in combination with methotrexate (MTX). The findings suggested that the combined therapy showed better efficacy in managing rheumatoid arthritis and reducing liver toxicity compared to MTX alone. Additionally, the leaf extract may serve as a natural anti-arthritic agent with fewer side effects on liver function.
Article
Chemistry, Multidisciplinary
Kaixiang Zhang, Yanrui Ma, Danyu Wang, Jingwen Liu, Jingyi An, Yanan Li, Chihong Ma, Yiran Pei, Zhenzhong Zhang, Junjie Liu, Jinjin Shi
Summary: An pH-driven interlocked DNA nano-spring was developed to stimulate T-cell activation in response to the low pH value in a tumor microenvironment. The spring-like shrinking of the nano-spring induced T-cell proliferation, leading to an enhanced antitumor effect.
Review
Immunology
Timothy N. J. Bullock
Summary: Advances in checkpoint blockade immunotherapies have led to an expansion in methods for promoting T cell access to the tumor microenvironment, with dendritic cells playing a crucial role in the immune response to tumor antigens. The role of CD40 as a master regulator of dendritic cell activation in cancer vaccines and other immune-oncology approaches is highlighted and evaluated.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yijiao Qu, Robert De Rose, Chan-Jin Kim, Jiajing Zhou, Zhixing Lin, Yi Ju, Sukhvir Kaur Bhangu, Christina Cortez-Jugo, Francesca Cavalieri, Frank Caruso
Summary: DNA-based particles were synthesized by supramolecular assembly of tannic acid (TA) and DNA, allowing control over morphology and bioactivity. The particles showed potential as gene and vaccine delivery platforms.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Pharmacology & Pharmacy
Yang Liu, Xingren Li, Xiuyin Wu, Xingyan Luo, Bingchao Yan, Chunfen Mo, Huijie Guo, Shuxia Yang, Yantang Wang, Yi Lai, Pematenzin Puno, Limei Li
Summary: The study found that Sis-25 exhibited significant immunosuppressive activity by targeting GSK3 beta both in vitro and in vivo, inhibiting multiple signaling pathways and cytokine expression, effectively suppressing immune responses and dermatitis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Immunology
Josephine R. Giles, Sasikanth Manne, Elizabeth Freilich, Derek A. Oldridge, Amy E. Baxter, Sangeeth George, Zeyu Chen, Hua Huang, Lakshmi Chilukuri, Mary Carberry, Lydia Giles, Nan-Ping P. Weng, Regina M. Young, Carl H. June, Lynn M. Schuchter, Ravi K. Amaravadi, Xiaowei Xu, Giorgos C. Karakousis, Tara C. Mitchell, Alexander C. Huang, Junwei Shi, E. John Wherry
Summary: This study generated an epigenetic and transcriptional atlas of T cell differentiation from healthy humans and applied it to explore disease-specific biology. The study identified molecular regulations of gene expression and chromatin accessibility during T cell differentiation and provided insights into disease biology through three research settings. The study also successfully predicted genome-wide cis-regulatory elements and validated the approach for functional annotation of key effector genes, demonstrating the potential of identifying targets for non-coding cellular engineering.
Article
Multidisciplinary Sciences
Hui-Fang Chang, Shih-En Chou, Ji-Yen Cheng
Summary: This study used a microfluidic cell culture system to investigate cell differentiation, finding that simple direct current pulse treatment can control the fate of neural stem and progenitor cells, aiding in the development of therapeutic strategies for nervous system disorders.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Engineering, Biomedical
Hye Sung Kim, Tzu-Chieh Ho, Moshe J. Willner, Michael W. Becker, Hae-Won Kim, Kam W. Leong
Summary: In this study, we propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells for adoptive cell therapy. The system utilizes microfiber scaffolds coated with dendritic cell membrane to replicate the physicochemical properties of dendritic cells, leading to greater expansion and functionality of T cells. This scalable and customizable platform holds promise for future research and application in cell therapy.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Analytical
Ryan C. Ashbaugh, Lalita Udpa, Ron R. Israeli, Assaf A. Gilad, Galit Pelled
Summary: Magnetogenetics is a new field that uses electromagnetic fields to remotely control cellular activity, requiring specific hardware designs to provide the necessary stimulation for electrophysiology and imaging experiments. A universal stimulus delivery system comprising four magnet designs compatible with different types of experiments has been developed, along with a low-cost stimulation controller for rapid switching and precise control of stimulation.
Article
Multidisciplinary Sciences
Zhen Li, Ping Yan, Rui Wang, Xiaofan Lu, Yang Zhang, Bin Su, Xin Zhang, Lin Yuan, Zhiying Liu, Wei Jiang, Tong Zhang, Hao Wu, Xiaojie Huang
Summary: HIV-1 infection leads to T cell dysfunction that cannot be fully restored by anti-retroviral therapy (ART). Myeloid-derived suppressor cells (MDSCs) expand and suppress T cell function during viral infection. In people with acute HIV-1 infection (PWAH) receiving early ART, T cells were hyper-activated and hyper-proliferative at pre-ART. Early ART normalized T cell activation but not proliferation.
Review
Chemistry, Physical
Johann Shane Tian, Andy Tay
Summary: With the persisting complexity of chronic diseases, there is a growing need to develop cell therapies as a solution. However, the shortage of starter cells and the time-consuming manufacturing process hinder accessibility to patients. This review discusses the importance of maximizing cell yield during manufacturing and explores strategies for modulating cell proliferation, specifically through electrical stimulation. Bridging the gap between academic investigation and industrial application is crucial for advancing cell therapy production.
Article
Biochemistry & Molecular Biology
Milica Perisic Nanut, Simon Zurga, Spela Konjar, Mateja Prunk, Janko Kos, Jerica Sabotic
Summary: Clitocybe nebularis lectin (CNL) exhibits antiproliferative effect on Jurkat leukemic T cell line by inducing homotypic aggregation and cell death. The cell death of Jurkat cells shows features of early apoptosis without caspase activation. Binding of CNL induces Jurkat cells to release the damage-associated molecular pattern molecule HMGB1. Another lectin with similar glycan-binding specificity, Wisteria floribunda agglutinin (WFA), also shows cytotoxicity and induces cell death in Jurkat cells.