期刊
INTERNATIONAL JOURNAL OF CANCER
卷 128, 期 10, 页码 2317-2325出版社
WILEY
DOI: 10.1002/ijc.25806
关键词
STAT5; leukemia stem cells; quiescence; AZ960
类别
资金
- Kochi University
- Setsuro Fujii Memorial
- Osaka Foundation for Promotion of Fundamental Medical Research
- Japan Society for the Promotion of Science (JSPS)
To verify molecular mechanisms by which leukemia stem cells (LSCs) maintain a dormant state, we explored the activity of the major prosurvival signal pathways in CD34(+)/CD38(-) compartment, supposed to contain LSCs, and CD34(+)/CD38(+) counterparts from patients with acute myelogenous leukemia (AML, n = 11) by fluorescence-activated cell sorting (FACS). CD34(+)/CD38(-) cells expressed a greater amount of p-janus kinase 2 (JAK2) and p-signal transducer and activator of transcription 5 (STAT5) than CD34(+)/CD38(+) counterparts in all patients except for one case. In addition, we found that CD34(+)/CD38(-) cells were relatively resistant to cytarabine- and the inhibitor of the fms-like tyrosine kinase 3 (FLT3)-mediated growth inhibition, as measured by the clonogenic assay. Interestingly, blockade of JAK2/STAT5 signaling by the specific JAK2 inhibitor AZ960 stimulated cell cycling in CD34(+)/CD38(-) cells in conjunction with downregulation of cyclin-dependent kinase inhibitor p21(waf1) and sensitized these cells to the growth inhibition mediated by cytarabine and the FLT3 kinase inhibitor. Moreover, exposure of CD34(+)/CD38(-) cells to AZ960 potently induced apoptosis in parallel with downregulation of antiapoptotic protein Bcl-xL, as measured by Western blot analysis. Taken together, JAK2/STAT5 signaling may be a promising molecular target to eradicate CD34(+)/CD38(-) leukemia cells in individuals with AML.
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