Article
Multidisciplinary Sciences
Munirah Alsaleh, Zoe Leftley, Thomas O'Connor, Thomas Hughes, Thomas A. Barbera, Larry K. Koomson, Abigail Zabron, Helen Reeves, Matthew Cramp, Stephen D. Ryder, Shaun Greer, Martin Prince, Paiboon Sithithaworn, Narong Khuntikeo, Watcharin Loilome, Puangrat Yongvanit, I. Jane Cox, Roger Williams, Christopher A. Wadsworth, Elaine Holmes, Kathryn Nash, Ross Andrews, Simon D. Taylor-Robinson
Summary: This study identified phenotypic diversity in urinary metabolomes of different geographical populations. Disparities in healthy metabolomes between two distinct populations were primarily related to differences in dietary practices and body composition, while metabolites excreted due to drug treatment were dominant in urine specimens from cholangiocarcinoma patients, particularly in Western individuals. Dysregulation in nucleotide metabolism associated with sporadic cholangiocarcinoma may indicate differences in mitochondrial energy production pathways between cholangiocarcinoma tumour subtypes, influenced by gut microbial community metabolism.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Paul J. Brindley, Melinda Bachini, Sumera I. Ilyas, Shahid A. Khan, Alex Loukas, Alphonse E. Sirica, Bin Tean Teh, Sopit Wongkham, Gregory J. Gores
Summary: Cholangiocarcinoma is a highly lethal adenocarcinoma of the hepatobiliary system that can be associated with liver fluke infection but often has no identifiable cause. This Primer reviews the epidemiology, pathophysiological mechanisms, diagnosis and management of cholangiocarcinoma, and highlights the patient experience and future directions.
NATURE REVIEWS DISEASE PRIMERS
(2021)
Article
Multidisciplinary Sciences
Nopparat Songserm, Somkiattiyos Woradet, Waratip Kankarn, Kanjanar Pintakham, Phouthong Vanhnivongkham, Nguyen Thi To Uyen, Nguyen Cong Cuu, Le Ngoc Cua, Banchob Sripa, Akhtar Ali
Summary: This study aims to investigate the prevalence of protective factors for cholangiocarcinoma (CCA) incidence and identify significant factors associated with prevention. The survey results show a relatively high prevalence of protective factors in the region, which is of great importance for both the public and administrators. Women, individuals with higher education, and those with a higher level of awareness regarding the seriousness and benefits of CCA prevention are significantly associated with CCA prevention.
Article
Parasitology
Sattrachai Prasopdee, Yodying Yingchutrakul, Sittiruk Roytrakul, Montinee Pholhelm, Teva Phanaksri, Anthicha Kunjantarachot, Jutharat Kulsantiwong, Kritiya Butthongkomvong, Smarn Tesana, Thanakrit Sathavornmanee, Veerachai Thitapakorn
Summary: In this study, potential biomarkers for Opisthorchis viverrini infection and cholangiocarcinoma were identified by analyzing the plasma phosphoproteomes of patients. The AKT signaling pathway was found to be up-regulated, and phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) was selected as a potential biomarker. The indirect enzyme-linked immunosorbent assay (ELISA) results showed that the levels of PIK3CB were significantly different in the OV and CCA groups compared to the non-OVCCA group, indicating its potential application in screening for opisthorchiasis and CHCA.
Article
Gastroenterology & Hepatology
Prakasit Sa-Ngiamwibool, Chaiwat Aphivatanasiri, Sakkarn Sangkhamanon, Piyapharom Intarawichian, Waritta Kunprom, Malinee Thanee, Piya Prajumwongs, Watcharin Loilome, Narong Khuntikeo, Attapol Titapun, Apiwat Jareanrat, Vasin Thanasukarn, Tharatip Srisuk, Vor Luvira, Kulyada Eurboonyanun, Julaluck Promsom, Aileen Wee, Supinda Koonmee
Summary: This study aimed to improve prognostic prediction and patient stratification for iCCA by creating the KKU staging system. The incorporation of growth patterns as independent prognostic factors and modification of AJCC stages in the KKU staging system resulted in better prediction of patient survival. The KKU staging system may serve as an alternative model for iCCA staging to enhance the accuracy of survival prognostication.
Article
Infectious Diseases
Rutchanee Rodpai, Vor Luvira, Lakkhana Sadaow, Wattana Sukeepaisarnjaroen, Amnat Kitkhuandee, Krisada Paonariang, Oranuch Sanpool, Wannaporn Ittiprasert, Victoria H. Mann, Pewpan M. Intapan, Paul J. Brindley, Wanchai Maleewong
Summary: By detecting anti-Opisthorchis antibodies in the sera of CCA patients, we found an association between Opisthorchis infection and CCA. This has potential value for the development of a diagnostic biomarker to predict risk for Opisthorchis infection-associated CCA.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Sattrachai Prasopdee, Yodying Yingchutrakul, Sucheewin Krobthong, Montinee Pholhelm, Patompon Wongtrakoongate, Kritiya Butthongkomvong, Jutharat Kulsantiwong, Teva Phanaksri, Anthicha Kunjantarachot, Thanakrit Sathavornmanee, Smarn Tesana, Veerachai Thitapakorn
Summary: This study aims to identify biomarkers for Opisthorchis viverrini infection and cholangiocarcinoma (CHCA), and successfully identifies and validates PIGR as a potential biomarker for CHCA.
Article
Oncology
Natcha Khuntikeo, Sureerat Padthaisong, Watcharin Loilome, Poramate Klanrit, Soontaree Ratchatapusit, Anchalee Techasen, Apiwat Jareanrat, Vasin Thanasukarn, Tharatip Srisuk, Vor Luvira, Jarin Chindaprasirt, Prakasit Sa-ngiamwibool, Chaiwat Aphivatanasiri, Piyapharom Intarawichian, Supinda Koonmee, Piya Prajumwongs, Attapol Titapun
Summary: This study found a high prevalence of dMMR in cholangiocarcinoma, and dMMR was identified as an independent prognostic factor for good survival, especially in early-stage CCA and for patients who received adjuvant chemotherapy.
Article
Parasitology
Jenwit Sornpom, Apiporn T. Suwannatrai, Kulwadee Suwannatrai, Matthew Kelly, Kavin Thinkhamrop
Summary: This study examines the relationship between misconceptions, unhealthy eating habits, and Opisthorchis viverrini infection in Northeast Thailand. The results suggest that individuals who believe in using lime or red ants in raw fish salad, or consuming raw fish with white whiskey, and those who think early-stage cholangiocarcinoma can be cured, are at a higher risk of O. viverrini infection.
PARASITOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Supot Kamsa-ard, Chalongpon Santong, Siriporn Kamsa-ard, Vor Luvira, Varisara Luvira, Krittika Suwanrungruang, Vajarabhongsa Bhudhisawasdi
Summary: This study used multiple statistical methods to analyze the incidence and survival rate trends of cholangiocarcinoma in northeastern Thailand. The results showed a decrease in the incidence rate of cholangiocarcinoma, with a projected continued decline in the future, though the survival rate of patients remains poor.
Article
Oncology
Nopparat Songserm, Sureerat Butprom, Comsun Thongchai, Monthicha Ruksilp, Pattaraporn Charoenbut, Somkiattiyos Woradet, Timith Souvanaa, Vanida Buonhoseng, Akhtar Ali
Summary: By training village health volunteers to educate locals on the prevention of cholangiocarcinoma, the program effectively reduced risk factors in the risk areas, showing promise for application in other regions.
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
(2022)
Article
Gastroenterology & Hepatology
Rungtiwa Dangtakot, Kitti Intuyod, Yaovalux Chamgramol, Chawalit Pairojkul, Somchai Pinlaor, Chanakan Jantawong, Thatsanapong Pongking, Ornuma Haonon, Ning Ma, Porntip Pinlaor
Summary: The study found that H. pylori infection and exposure to carcinogens can induce the development of cholangiocarcinoma (CCA) with slow progression.
Article
Immunology
Wuttipong Phumrattanaprapin, Mark Pearson, Darren Pickering, Bemnet Tedla, Michael Smout, Sujittra Chaiyadet, Paul J. Brindley, Alex Loukas, Thewarach Laha
Summary: The study showed that monoclonal antibodies against Ov-TSP-2 can protect against opisthorchiasis in hamsters, indicating the potential of antibody-directed therapies for liver fluke infections. This supports the use of TSPs as components of a subunit vaccine for opisthorchiasis and similar infections.
Article
Oncology
Teva Phanaksri, Yodying Yingchutrakul, Sittiruk Roytrakul, Sattrachai Prasopdee, Anthicha Kunjantarachot, Kritiya Butthongkomvong, Smarn Tesana, Thanakrit Sathavornmanee, Veerachai Thitapakorn
Summary: This study identified the upregulation of plasma checkpoint protein 1 (Chk1) in plasma samples from opisthorchiasis viverrini-infected and cholangiocarcinoma-derived individuals, suggesting its potential as a diagnostic biomarker for these conditions.
Article
Cell Biology
Piyanard Boonnate, Kulthida Vaeteewoottacharn, Ryusho Kariya, Sawako Fujikawa, Thidarut Boonmars, Somchai Pinlaor, Chawalit Pairojkul, Seiji Okada
Summary: A new hamster cholangiocarcinoma cell line, Ham-2, was established and characterized for its aggressive phenotypes in vitro and in vivo. This cell line could serve as a valuable tool for comparative drug target identification and validation in CCA research.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2021)
Article
Parasitology
Wannee Kaewrat, Chatchawan Sengthong, Manachai Yingklang, Kitti Intuyod, Ornuma Haonon, Sudarat Onsurathum, Rungtiwa Dangtakot, Phitsamai Saisud, Arunnee Sangka, Sirirat Anutrakulchai, Somchai Pinlaor, Ubon Cha'on, Porntip Pinlaor
Article
Biochemistry & Molecular Biology
Raynoo Thanan, Waleeporn Kaewlert, Chadamas Sakonsinsiri, Timpika Chaiprasert, Napat Armartmuntree, Duangkamon Muengsaen, Anchalee Techasen, Poramate Klanrit, Worachart Lert-itthiporn, Somchai Pinlaor, Chawalit Pairojkul
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Parasitology
Chatchawan Sengthong, Manachai Yingklang, Kitti Intuyod, Nuttanan Hongsrichan, Somchai Pinlaor
PARASITOLOGY RESEARCH
(2020)
Article
Multidisciplinary Sciences
Thatsanapong Pongking, Ornuma Haonon, Rungtiwa Dangtakot, Sudarat Onsurathum, Apinya Jusakul, Kitti Intuyod, Arunnee Sangka, Sirirat Anutrakulchai, Ubon Cha'on, Somchai Pinlaor, Porntip Pinlaor
Article
Parasitology
Yi-Chen Wang, Timothy Zherui Liew, Jutamas Namsanor, Paiboon Sithithaworn
PARASITOLOGY RESEARCH
(2020)
Review
Parasitology
Thomas Crellen, Paiboon Sithithaworn, Opal Pitaksakulrat, Narong Khuntikeo, Graham F. Medley, T. Deirdre Hollingsworth
Summary: This article discusses the ongoing transmission of the carcinogenic liver fluke Opisthorchis viverrini in Southeast Asia, the different stages of control in endemic countries within the region, and the lack of evidence on effective interventions for reducing parasite transmission and liver cancer. The use of quantitative modeling to evaluate control measures and assist in designing clinical trials is proposed as a way to develop evidence-based strategies for parasite control at a national or regional level.
TRENDS IN PARASITOLOGY
(2021)
Article
Parasitology
Naruemon Bunchom, Chairat Tantrawatpan, Takeshi Agatsuma, Narufumi Suganuma, Warayutt Pilap, Warong Suksavate, Paiboon Sithithaworn, Trevor N. Petney, Ross H. Andrews, Weerachai Saijuntha
Summary: This study investigated the geographical distribution and genetic structure of Bithynia species in Thailand, using COI and 16S rDNA sequences. The results indicated that B. funiculata is found only in the north of Thailand, while B. s. goniomphalos contains three distinct lineages and B. s. siamensis is classified into one lineage. Results suggest reclassification of B. s. goniomphalos and B. s. siamensis as a species complex Bithynia siamensis sensu lato.
Article
Parasitology
Sirowan Ruantip, Chatanun Eamudomkarn, Kulthida Y. Kopolrat, Jiraporn Sithithaworn, Thewarach Laha, Paiboon Sithithaworn
Summary: Recent research has shown that urine analysis is as sensitive as serology in diagnosing strongyloidiasis. The study found that urine ELISA for Strongyloides-specific IgG had higher prevalence rates compared to parasitological methods, with minimal daily variation over consecutive days. Urine ELISA is a promising method for clinical diagnosis and screening of strongyloidiasis in populations.
Article
Urology & Nephrology
Pantipa Tonsawan, Sompote Intarak, Banchob Sripa, Anucha Puapairoj, Manop Sripa, Paiboon Sithithaworn, Sirirat Anutrakulchai
Summary: This study is the first to demonstrate the presence of Opisthorchis viverrini (OV) antigen in human kidney tissue, suggesting a possible association between OV infection and biopsy-proven glomerular diseases.
AMERICAN JOURNAL OF NEPHROLOGY
(2022)
Article
Microbiology
Natchita Chungkanchana, Paiboon Sithithaworn, Chanika Worasith, Phattharaphon Wongphutorn, Sirowan Ruantip, Kulthida Y. Kopolrat, Apinya Jusakul, Raynoo Thanan, Kunyarat Duenngai, Jiraporn Sithithaworn, Anchalee Techasen
Summary: This study evaluated the effects of urine concentration on the detection of parasite-specific IgG by urine enzyme-linked immunosorbent assay (ELISA). The results showed that the concentration of urine samples prior to analysis improved the sensitivity for diagnosis of strongyloidiasis. The concentrated-urine ELISA increased the proportion of positive results in individuals with borderline results or false-negative diagnosis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Parasitology
Kulthida Y. Kopolrat, Paiboon Sithithaworn, Nadda Kiatsopit, Jutamas Namsanor, Opal Pitaksakulrat, Patiwat Yasaka, Prasert Saichua, Weerachai Saijuntha, Smarn Tesana, Ross H. Andrews, Trevor N. Petney
Summary: The study revealed that Bithynia siamensis goniomphalos serves as an intermediate host for various trematodes, with virgulate xiphidiocercariae and Opisthorchis viverrini being the most prevalent. The quantity of irrigation water for rice cultivation was found to be correlated with the transmission dynamics of trematodes in B. s. goniomphalos, impacting the peak prevalence of trematode infections in the snails. Presence of other trematodes in the snail was found to negatively impact the emergence of virgulate cercariae, highlighting the complex interactions between host and parasite in the transmission dynamics.
Article
Biology
Thomas Crellen, Melissa Haswell, Paiboon Sithithaworn, Somphou Sayasone, Peter Odermatt, Poppy H. L. Lamberton, Simon E. F. Spencer, T. Deirdre Hollingsworth
Summary: A study on opisthorchiasis found that parasite fecundity varies between populations and the relationship between routine diagnostics and true prevalence is influenced by parasite aggregation.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chatanun Eamudomkarn, Sirowan Ruantip, Jiraporn Sithithaworn, Anchalee Techasen, Kulthida Y. Kopoolrat, Chanika Worasith, Phattharaphon Wongphutorn, Jeffrey M. Bethony, Thewarach Laha, Paiboon Sithithaworn
Summary: Detection of anti-Strongyloides IgG in urine by ELISA showed comparable performance to conventional serum assays for diagnosing strongyloidiasis. Optimized urine ELISA protocols using different antigens yielded higher prevalence rates and showed potential as alternative diagnostic methods. The results supported the utility of urine specimens for the diagnosis of strongyloidiasis.
Article
Agriculture, Dairy & Animal Science
Chairat Tantrawatpan, Wanchai Maleewong, Tongjit Thanchomnang, Warayutt Pilap, Takeshi Agatsuma, Ross H. Andrews, Paiboon Sithithaworn, Weerachai Saijuntha
Summary: This study aimed to characterize the intron regions of taurocyamine kinase gene (TK) in Opisthorchis viverrini and Clonorchis sinensis, and investigate their genetic variation and population genetics. The researchers found high nucleotide and haplotype diversity in O. viverrini samples, and observed heterozygous patterns specifically in humans with C. sinensis. They suggested that TkD1Int5 could be a high polymorphic genetic marker for further population genetic investigations of these liver flukes and related species.
Article
Parasitology
O. Pitaksakulrat, P. Sithithaworn, K. Y. Kopolrat, N. Kiatsopit, W. Saijuntha, R. H. Andrews, T. N. Petney, D. Blair
Summary: Digenetic trematodes are important parasites of humans and animals, infecting a gastropod as the first intermediate host. The morphological details of cercariae provide limited identification, so molecular data was used to improve identification and investigate the diversity of trematode species infecting B. s. goniomphalos in Thailand.
JOURNAL OF HELMINTHOLOGY
(2022)
