Article
Biochemistry & Molecular Biology
Tereza Koranova, Lukas Dvoracek, Dana Grebenova, Pavla Roselova, Adam Obr, Katerina Kuzelova
Summary: Inhibiting or deleting members of the P21-activated kinases (PAKs) family can impact cell metabolism, influencing mitochondrial respiration and aerobic glycolysis rates. Specifically, downregulation of PAK2 is linked to oxidative phosphorylation, while knockout of PAK1 results in increased glycolysis. Despite these changes, the overall metabolic capacity is not substantially reduced, possibly due to redundancy or compensatory mechanisms within the PAK1/PAK2 regulatory roles.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Preety Bajwa, Kasjusz Kordylewicz, Agnes Bilecz, Ricardo R. Lastra, Kristen Wroblewski, Yuval Rinkevich, Ernst Lengyel, Hilary A. Kenny
Summary: Ovarian cancer preferentially metastasizes in association with mesothelial cell-lined surfaces. The presence of mesothelial cells is required for ovarian cancer metastasis, and the interaction between mesothelial cells and ovarian cancer cells leads to alterations in gene expression and cytokine secretion. The secretion of ANGPTL4 by mesothelial cells promotes ovarian cancer metastasis through various mechanisms.
Article
Cell Biology
Rong Jiang, Hongyu Zhang, Jinhua Zhou, Juan Wang, Yuejuan Xu, He Zhang, Yanzheng Gu, Fengqing Fu, Yu Shen, Guangbo Zhang, Lanlan Feng, Xueguang Zhang, Youguo Chen, Fangrong Shen
Summary: XIST and FOXP3 were upregulated, while miR-149-3p was downregulated in OC tissues and cells. High XIST expression indicated a poor prognosis for OC. Inhibition of XIST or elevation of miR-149-3p suppressed malignant behaviors of OC cells, such as proliferation, invasion, migration, and colony formation, and promoted apoptosis and cell cycle arrest. This study suggests that knockdown of XIST increases miR-149-3p expression to inhibit OC development.
CELL DEATH & DISEASE
(2021)
Article
Biochemical Research Methods
Aydanur Senturk, Ayse T. Sahin, Ayse Armutlu, Murat Can Kiremit, Omer Acar, Selcuk Erdem, Sidar Bagbudar, Tarik Esen, Nurhan Ozlu
Summary: In this study, quantitative phosphoproteomics characterization of ccRCC tumor and normal adjacent tissues revealed significant differential regulation of phosphoproteins between tumor and normal tissues. These differentially regulated phosphoproteins are associated with proliferative and migratory behavior of renal tumors. Furthermore, master kinases responsible for phosphorylation of substrates associated with cell proliferation, inflammation, and migration were identified.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biology
Joseph O. Magliozzi, James B. Moseley
Summary: This study reveals a new role for Pak1 in regulating cell shape through its association with the RNA-binding protein Sts5 and P bodies. Pak1 plays a key role in preventing Sts5 from associating with P bodies and promotes rapid dissolution of Sts5 during glucose addition by localizing to stress granules.
Article
Oncology
Leilei Liang, Jian Li, Jing Yu, Jing Liu, Lin Xiu, Jia Zeng, Tiantian Wang, Ning Li, Lingying Wu
Summary: This study established an invasion-related multigene signature to predict the prognostic risk of ovarian cancer. A 6-gene prognostic risk model was constructed and shown to be independent of clinical features. This signature has the potential to evaluate the prognostic risk of ovarian cancer patients.
CANCER CELL INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Linfeng Mao, Weijie Yuan, Kaimei Cai, Chen Lai, Changhao Huang, Yi Xu, Shangwei Zhong, Chen Yang, Ran Wang, Pengwei Zeng, Heyuan Huang, Zhikang Chen, Zihua Chen
Summary: EphA2, YES1, and ANXA2 form a signaling axis that promotes gastric cancer invasion and metastasis. Overexpression of YES1 increases invasion and migration of gastric cancer cells, while knockdown of ANXA2 decreases these processes.
Article
Mathematical & Computational Biology
Yan Song, Juan Zhang, Lei Zhang, Suxia Zhang, Chengcheng Shen
Summary: PDP1 is overexpressed in ovarian cancer tissues and is associated with poor prognosis and worse clinical parameters. In vitro experiments show that PDP1 promotes cell proliferation, invasion, and migration in ovarian cancer cells. GSEA analysis reveals that the IL6/JAK/STAT3 signaling pathway, interferon-alpha response, apoptosis, adipogenesis, KRAS signaling, and IL2/STAT5 signaling are activated in ovarian cancer patients with high PDP1 expression. Drug sensitivity analysis demonstrates a negative correlation between PDP1 expression and the IC50 of bleomycin and gemcitabine, indicating that high PDP1 expression may lead to increased sensitivity to these drugs and decreased sensitivity to cisplatin. PDP1 plays a role in ovarian cancer progression and is a potential biomarker for patient prognosis and chemosensitivity.
COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yi-Ping Tao, Heng-Yan Zhu, Qian-Yuan Shi, Cai-Xia Wang, Yu-Xin Hua, Han-Yin Hu, Qi-Yin Zhou, Zi-Lu Zhou, Ying Sun, Xiao-Min Wang, Yu Wang, Ya-Ling Zhang, Yan-Jun Guo, Zi-Ying Wang, Xuan Che, Chun-Wei Xu, Xian-Chao Zhang, Michal Heger, Su-Ping Tao, Xin Zheng, Ying Xu, Lei Ao, Ai-Jun Liu, Sheng-Bing Liu, Shu-Qun Cheng, Wei-Wei Pan
Summary: S1PR1 was found to be highly expressed in human ovarian cancer tissues and cells, and its deletion inhibited cancer cell proliferation and migration while promoting cell senescence and sensitivity to chemotherapy. S1P was shown to regulate ovarian cancer cell senescence by modulating the expression of PDK1, LATS1/2, and YAP. The study demonstrates the importance of the S1PR1-PDK1-LATS1/2-YAP signaling axis in the regulation of ovarian cancer cell senescence.
Article
Multidisciplinary Sciences
Anette M. Skjervold, Marit Valla, Borgny Ytterhus, Anna Bofin
Summary: This study aimed to assess the association between PAK1 gene copy number and proliferation status, molecular subtype, and prognosis in breast cancer. The results showed that PAK1 copy number increase is associated with high proliferation and high histological grade, but not with prognosis. PAK1 copy number increase is most frequent in the HER2 type and Luminal B (HER2(-)) subtype and is associated with CCND1 copy number increase.
Article
Oncology
Xiu Shi, Xuejiao Yu, Juan Wang, Shimin Bian, Qiutong Li, Fengqing Fu, Xinwei Zou, Lin Zhang, Robert C. Bast, Zhen Lu, Lingchuan Guo, Youguo Chen, Jinhua Zhou
Summary: Salt-inducible kinase 2 (SIK2) is overexpressed in ovarian cancer and promotes cancer cell motility and metastasis through the regulation of myosin light chain kinase. Adipocytes induce the phosphorylation of SIK2 and myosin light chain kinase, enhancing the migration of ovarian cancer cells. The expression of SIK2 is positively correlated with MYLK-pS343 and is associated with reduced overall survival in ovarian cancer patients. SIK2 could be a potential candidate for ovarian cancer treatment.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Lili Zhang, Huixiao Chen, Fengxi He, Shiqian Zhang, Aihua Li, Aifeng Zhang, Anqi Zhang
Summary: miR-320a plays an important role in ovarian cancer progression and infiltration, promoting the proliferation, migration, and invasion of EOC cells by targeting RASSF8.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Liu Yang, Xing Du, Siqi Wang, Chenggang Lin, Qiqi Li, Qifa Li
Summary: miR-187 maintains TGFBR2 mRNA stability in sow GCs by activating the TGF-beta signaling pathway and inhibiting GC apoptosis. NORHA inhibits the activation of TGF-beta signaling pathway by sponging miR-187. NORFA enhances miR-187 and TGFBR2 expression by inhibiting NORHA and activating NFIX.
CELL DEATH DISCOVERY
(2023)
Article
Oncology
Lucile Yart, Daniel Bastida-Ruiz, Mathilde Allard, Pierre-Yves Dietrich, Patrick Petignat, Marie Cohen
Summary: This study found that cell fusion may be involved in the formation of ovarian PGCCs, and this process is promoted by paclitaxel and UPR activation. Modulating the UPR could be an interesting therapeutic strategy to prevent the formation of PGCCs and therefore limit cancer relapse and drug resistance.
Article
Medicine, Research & Experimental
Tianshui Sun, Fangfang Bi, Zhuonan Liu, Qing Yang
Summary: In ovarian cancer patients, TMEM119 is overexpressed and associated with poor survival. Experimental results indicate TMEM119 promotes proliferation, invasion, and migration of ovarian cancer cells. Additionally, TMEM119 may exert oncogenic effects by regulating PDGFRB expression and activating the PI3K/AKT signaling pathway.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Pathology
Ying Yu, Ka-Yu Tse, Horace H. Y. Lee, Kin-Long Chow, Hing-Wai Tsang, Richard W. C. Wong, Elaine T. Y. Cheung, Wah Cheuk, Victor W. K. Lee, Wai-Kong Chan, Alice S. T. Wong, Herbert H. F. Loong, Karen K. L. Chan, Hextan Y. S. Ngan, Annie N. Y. Cheung, Philip P. C. Ip
Article
Pathology
Ava Kwong, Vivian Y. Shin, Jiawei Chen, Isabella W. Y. Cheuk, Cecilia Y. S. Ho, Chun H. Au, Karen K. L. Chan, Hextan Y. S. Ngan, Tsun L. Chan, James M. Ford, Edmond S. K. Ma
JOURNAL OF MOLECULAR DIAGNOSTICS
(2020)
Article
Radiology, Nuclear Medicine & Medical Imaging
Elaine Y. P. Lee, He An, Jose A. U. Perucho, Keith W. H. Chiu, Edward S. Hui, Mandy M. Y. Chu, Hextan Y. S. Ngan
EUROPEAN RADIOLOGY
(2020)
Article
Reproductive Biology
Tung Tung Tsoi, Keith W. H. Chiu, M. Y. Chu, Hextan Y. S. Ngan, Elaine Y. P. Lee
JOURNAL OF OVARIAN RESEARCH
(2020)
Article
Oncology
Karen Kar Loen Chan, Siew Fei Ngu, Mandy Man Yee Chu, Ka Yu Tse, Hextan Yuen Sheung Ngan
Summary: A retrospective review of tamoxifen use in patients with advanced or recurrent ovarian cancer showed a clinical benefit rate of 56% and a median progression-free survival of 5.3 months. Patients tolerated tamoxifen well, and hormone receptor status was not predictive of response. Tamoxifen may still have a role in the era of molecular target therapy for these patients.
ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Genetics & Heredity
Mullin Ho Chung Yu, Jeffrey Fong Ting Chau, Sandy Leung Kuen Au, Hei Man Lo, Kit San Yeung, Jasmine Lee Fong Fung, Christopher Chun Yu Mak, Claudia Ching Yan Chung, Kelvin Yuen Kwong Chan, Brian Hon Yin Chung, Anita Sik Yau Kan
Summary: Balanced chromosomal abnormalities (BCAs) are changes in chromosomal segments without genetic material gain or loss, occurring in 1 in 500 newborns and associated with increased risk of congenital anomalies and neurodevelopmental disorders. Using short read genome sequencing (GS) for diagnosing BCAs can provide more specific genetic diagnosis information, potentially improving genetic counseling and perinatal management.
FRONTIERS IN GENETICS
(2021)
Article
Genetics & Heredity
Florrie N. Y. Yu, Elizabeth Y. Y. Li, Meliza C. W. Kong, Teresa W. L. Ma, Kelvin Y. K. Chan, Elim Man, Brian H. Y. Chung, Anita S. Y. Kan
Summary: The incidence of discordant fetal sex is estimated to be 1 in 1500-2000, requiring comprehensive evaluation. Chimera is identified as one of the possible causes of genotype-phenotype sex discordance, detectable prenatally through a combination of noninvasive prenatal screening and ultrasound. Genetic counseling for prospective parents on chimera may be complex, necessitating early support and information from experienced professionals.
PRENATAL DIAGNOSIS
(2021)
Article
Genetics & Heredity
Pui-Tak Yu, Wendy Shu, Sau-Lan Mok, Pui-Wah Hui, Lin-Wai Chan, Ka-Yin Kwok, Kelvin Y. K. Chan, Tsz-Kin Lo, Brian H. Y. Chung, Ho-Ming Luk, Anita S. Y. Kan
Summary: Beckwith-Wiedemann Syndrome (BWS) is an imprinting disorder characterized by a range of prenatal features. Paternal uniparental disomy in chromosome 11p15 imprinting region is a common cause of BWS, while genome-wide paternal uniparental disomy (GWpUPD) is rare and typically results in prenatal lethality. We reported two prenatal cases initially presenting with BWS features, which were confirmed to have GWpUPD. SNP genotyping analysis revealed androgenetic biparental chimera as the underlying cause.
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2022)
Article
Genetics & Heredity
Jeffrey Fong Ting Chau, Mullin Ho Chung Yu, Martin Man Chun Chui, Cyrus Chun Wing Yeung, Aaron Wing Cheung Kwok, Xuehan Zhuang, Ryan Lee, Jasmine Lee Fong Fung, Mianne Lee, Christopher Chun Yu Mak, Nicole Ying Ting Ng, Claudia Ching Yan Chung, Marcus Chun Yin Chan, Mandy Ho Yin Tsang, Joshua Chun Ki Chan, Kelvin Yuen Kwong Chan, Anita Sik Yau Kan, Patrick Ho Yu Chung, Wanling Yang, So Lun Lee, Godfrey Chi Fung Chan, Paul Kwong Hang Tam, Yu Lung Lau, Kit San Yeung, Brian Hon Yin Chung, Clara Sze Man Tang
Summary: This study analyzed genetic sequencing data from the Southern Chinese population and found that approximately half of the population are carriers of one or more recessive genetic disorders, with a portion of carriers having treatable inherited conditions. The findings can inform carrier screening recommendations for the Southern Chinese population.
NPJ GENOMIC MEDICINE
(2022)
Article
Genetics & Heredity
Mianne Lee, Anna K. Y. Kwong, Martin M. C. Chui, Jeffrey F. T. Chau, Christopher C. Y. Mak, Sandy L. K. Au, Hei Man Lo, Kelvin Y. K. Chan, Vicente A. Yepez, Julien Gagneur, Anita S. Y. Kan, Brian H. Y. Chung
Summary: This study demonstrates the feasibility and benefits of using amniotic fluid cells for RNA sequencing in prenatal diagnosis, providing functional support for interpreting variants of uncertain significance and offering a non-invasive option for postnatal patients.
NPJ GENOMIC MEDICINE
(2022)
Article
Oncology
Shakeel Ahmad Khan, Kelvin Yuen Kwong Chan, Terence Kin Wah Lee
Summary: This scientometric study provides a comprehensive overview of the global research landscape of Metronomic Chemotherapy from 2000 to 2022. It identifies key trends, collaborations, and potential opportunities using a data-driven approach. The United States, Italy, and China are the major contributors to MC research, while there is a lack of collaborative efforts between countries and organizations. The study highlights emerging interdisciplinary research areas and influential authors, institutions, and journals.
Article
Health Care Sciences & Services
Theodora Hei Tung Lai, Leung Kuen Sandy Au, Yuen Ting Eunice Lau, Hei Man Lo, Kelvin Yuen Kwong Chan, Ka Wang Cheung, Teresa Wei Ling Ma, Wing Cheong Leung, Choi Wah Kong, Wendy Shu, Po Lam So, Anna Ka Yee Kwong, Christopher Chun Yu Mak, Mianne Lee, Martin Man Chun Chui, Brian Hon Yin Chung, Anita Sik Yau Kan
Summary: The study demonstrates the utility of whole-exome sequencing (WES) in prenatal diagnosis of fetal structural congenital abnormalities (SCAs) in Hong Kong. The highest diagnostic rate was found in fetuses with multiple SCAs, particularly involving the cardiac and musculoskeletal systems. The use of WES should be recommended in addition to conventional genetic workup.
Article
Radiology, Nuclear Medicine & Medical Imaging
He An, Jose A. U. Perucho, Keith W. H. Chiu, Edward S. Hui, Mandy M. Y. Chu, Siew Fei Ngu, Hextan Y. S. Ngan, Elaine Y. P. Lee
Summary: This study investigated the association between functional tumor burden of peritoneal carcinomatosis derived from DWI and overall survival in patients with advanced ovarian carcinoma. The results showed that a high DWI-derived functional tumor burden was associated with decreased overall survival.
KOREAN JOURNAL OF RADIOLOGY
(2022)
Article
Medicine, General & Internal
B. H. Y. Chung, A. S. Y. Kan, K. Y. K. Chan, W. Yang, M. H. Y. Tang, C. C. Y. Mak, G. K. C. Leung
HONG KONG MEDICAL JOURNAL
(2022)
Review
Genetics & Heredity
Mimi Tin-Yan Seto, Aida M. Bertoli-Avella, Ka Wang Cheung, Kelvin Yuen-Kwong Chan, Kit San Yeung, Jasmine Lee-Fong Fung, Christian Beetz, Peter Bauer, Ho Ming Luk, Ivan Fai-Man Lo, Chin Peng Lee, Brian Hon-Yin Chung, Anita Sik-Yau Kan
Summary: Schuurs-Hoeijmakers syndrome (SHS) is a rare syndrome caused by a de novo variant in the PACS1 gene. It is characterized by typical facial features, developmental delay, intellectual disability, and multiple malformations, with distinct diagnostic features before and after birth. Use of exome sequencing has aided in diagnosing SHS and expanding the understanding of the clinical phenotype associated with pathogenic PACS1 variants.
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2021)
