期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 9, 期 10, 页码 1070-1078出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.6662
关键词
Mesenchymal stem cells (MSCs); cyst; periapical inflammatory tissues; mesenchymal stem cells isolated from human periapical cyst (hPCy-MSCs); osteogenic differentiation; adipogenic differentiation; odontogenic cyst
资金
- PROMETEO Project - Progettazione e Sviluppo di piattaforme tecnologiche innovative ed ottimizzazione di PROcessi per applicazioni in MEdicina rigenerativa in ambito oromaxillofaciale, emaTologico, nEurologico e cardiOlogico [PON01_02834]
We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73(+), CD90(+), CD105(+), CD13(+), CD29(+), CD44(+), CD45(-), STRO-1(+), CD146(+)) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast-and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.
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