期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 65, 期 -, 页码 97-106出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2014.01.021
关键词
Antioxidant; Chitosan; Caffeic acid; Ferulic acid; In vitro; In vivo
资金
- National Natural Science Foundation of China [31101216]
- Natural Science Foundation of the Education Committee of Jiangsu province [11KJB550006]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
In this study, two water soluble chitosan derivatives were synthesized by grafting caffeic acid (CA) and ferulic acid (FA) onto chitosan via a free radical mediated method. The structural characterization, antioxidant activity in vitro and in vivo of chitosan derivatives were determined. Results showed that the UV-vis absorption peaks of chitosan derivatives shifted toward longer wavelengths. FT-IR spectroscopy exhibited the typical phenolic characteristics within 1450-1600 cm(-1). H-1 NMR spectroscopy showed new peaks of phenyl protons at 6.2-7.6 ppm. C-13 NMR spectroscopy showed additional peaks between 110 and 150 ppm assigned to the C=C of phenolic groups. These results all confirmed the successful grafting of CA and FA onto chitosan backbones. The chitosan derivatives had decreased thermal stability and crystallinity as compared to chitosan. In vitro assays showed that the antioxidant activity decreased in the order of CA-g-chitosan > FA-g-chitosan > chitosan. Moreover, administration of the chitosan derivatives could significantly increase antioxidant enzymes activities and decrease malondialdehyde levels in both serums and livers of D-galactose induced aging mice. Our results indicated the potential of CA-g-chitosan and FA-g-chitosan in the development of novel antioxidant agents. (C) 2014 Elsevier B.V. All rights reserved.
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