期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 67, 期 -, 页码 303-311出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2014.03.019
关键词
Ecklonia cava; Sulfate-fucans; Immune-enhancing; NF-kappa B; MAPK
资金
- Marine Technology Application Program of Ministry of Oceans and Fisheries, Republic of Korea
Water-soluble sulfated fucans isolated from Ecklonia cava were fractionated using an anion-exchange chromatography to investigate their molecular characteristics and immunomodulating activities. The crude fucoidan extract and purified fractions (EF1, EF2, and EF3) consisted mostly of different ratios of neutral sugars, proteins, sulfates, uronic acids, and their monosaccharide compositions were also significantly different. The backbone of the most immunoenhancing fraction, EF2, was mainly linked by (1 -> 3)-linked fucopyranosyl and (1 -> 4)-linked mannopyranosyl residues with sulfates at C-4 of fucopyranosyl units. The molecular weights of the crude fucoidan extract and purified fractions ranged from 8.3 x 10(3) to 442.6 x 10(3) g/mol. The crude extract, EF1 and EF2 stimulated RAW264.7 cells to produce considerable amounts of nitric oxide and cytokines. The treatment of cells with the sulfated fucans induced the degradation of 1 kappa-B and the phosphorylation of MAPK in RAW264.7 cells, implying that they might stimulate RAW264.7 cells through the activation of NF-kappa B and MAPK pathways. (C) 2014 Elsevier B.V. All rights reserved.
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