期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 52, 期 -, 页码 184-191出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2012.09.011
关键词
Pueraria lobata; Dendritic cell maturation; MAPKs; NK-kappa B; TLR4
资金
- MRC program [2010-0029480]
- Happy Tech. program through the National Research Foundation of Korea (NRF) [2011-0020971]
Maturation of dendritic cells (DCs) is a critical factor for initiating the immune response. However, DC maturation is usually attenuated in the tumor microenvironment, which is an important immunological problem in DC-based immunotherapy against cancer. Here, we report the effect of a polysaccharide (PLP) isolated from Pueraria lobate on phenotypic and functional maturation of DCs. Phenotypic maturation was demonstrated by increased expression of CD40, CD86, and major histocompatibility complex I/II. PLP induced functional maturation of DCs, as shown by increased production of interleukin (IL)-12, IL-1 beta, and tumor necrosis factor-alpha, decreased antigen capture capacity, and enhanced allogenic T cell stimulation. In addition, PLP activated DCs generated from C3H/HeJ mice with normal TLR4, but not DCs from C3H/HeJ mice with mutated TLR4, suggesting that the TLR4 is a membrane receptor of PLP. We showed that PLP increased ERK, JNK, and p38 mitogen-activated protein kinase phosphorylation, and nuclear translocation of the nuclear factor-kappaB p65 subunit, which are signaling molecules downstream of TLR4. These results indicate that PLP induced DC maturation through TLR4 signaling. (c) 2012 Elsevier B.V. All rights reserved.
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