期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 50, 期 4, 页码 1158-1164出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2012.01.007
关键词
Chitin; 6-Carboxychitin; Heparin-like; Sulfation; Anticoagulant activity
资金
- Key Laboratory of Fermentation Engineering (Ministry of Education) Open Foundation [2010KFJJ04]
- Natural Science Foundation of Hubei [2006ABA247]
- Research Foundation of Changzhou University [ZMF11020018]
A series of heparin-like 6-carboxylchitin derivatives with different N-acetyl group and sulfate group contents were prepared. Their structures were characterized by element analysis, FT-IR, C-13 NMR, and gel permeation chromatography. Their anticoagulant activity in vitro was investigated for human plasma with respect to activated partial thromboplastin time (APTT). The results showed all 6-carboxylchitin derivatives prolonged APTT within the scope of studied degree of sulfation (0.28-1.03) and Mws (4.3-13.7 kDa). Their anticoagulant activity strongly depended on their structures. 3,6-O-sulfated group promoted the anticoagulant activity. Only incorporation of N-sulfated group into deacetylated 6-carboxylchitin could not improve the anticoagulant activity. But, N-sulfated group and O-sulfated group had the synergistic action, and N-sulfated group could promote the anticoagulant activity for the N,O-sulfated chitin derivatives. In addition, acetyl group took a role in the anticoagulant activity, too. (C) 2012 Elsevier B.V. All rights reserved.
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