Article
Biochemistry & Molecular Biology
Francesca Camera, Eleonora Colantoni, Tomas Garcia-Sanchez, Barbara Benassi, Claudia Consales, Adeline Muscat, Leslie Vallet, Luis M. Mir, Franck Andre, Caterina Merla
Summary: In recent years, the application of nanosecond pulsed electric fields has been investigated for novel medical purposes. This study focused on examining molecular calcium fluxes induced by different fractionated protocols of short electric pulses. The researchers found that fractionated doses of nanosecond pulsed electric fields did not increase cell electrosensitization, but targeted activation of calcium-dependent genes was observed based on the duration of calcium flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Dagmar Ammelt, Alica Lammerskitten, Artur Wiktor, Francablisco J. Barba, Stefan Toepfl, Oleksii Parniakov
Summary: The study investigated the impact of pulsed electric fields (PEF) on the cell disintegration index (Zp), freeze-drying process, and final product quality of red beet and pineapple tissue. While it did not reduce drying time, PEF pre-treatment significantly enhanced the quality of the freeze-dried product. The effectiveness of PEF was found to be dependent on the type of plant material used.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Daniele Carullo, Biresaw Demelash Abera, Mariarosa Scognamiglio, Francesco Donsi, Giovanna Ferrari, Gianpiero Pataro
Summary: In this study, a cascaded cell disintegration process using pulsed electric fields and high-pressure homogenization was developed to efficiently and selectively release intracellular compounds. The results showed that the cascaded treatment improved the selectivity of extraction and increased the yields of carbohydrates and lipids, while maintaining a high yield of water-soluble proteins.
Article
Chemistry, Multidisciplinary
Mafalda Aguiar-Macedo, Luis M. M. Redondo, Marcos Teotonio Pereira, Carlos Silva
Summary: The increase in awareness of sustainable food production and consumption motivates the development of innovative technologies to improve efficiency and reduce production factors. Nonthermal pulsed electric fields (PEFs) show promise in optimizing processes without compromising product quality. In this study, the impact of commercial-grade enzymes and PEF treatment on white grape must and wine yield and quality was compared. PEFs resulted in the highest increase in wine yield and a significant reduction in wine lees production. The effects of PEFs on various oenological parameters were analyzed and compared with control and enzymatic treatments. The direct costs of PEF and enzyme usage were also determined, with PEFs being more cost-effective.
APPLIED SCIENCES-BASEL
(2023)
Article
Thermodynamics
Yong Tang, Qiang Yao, Marien Simeni Simeni, Igor V. Adamovich
Summary: This study investigates the response of counterflow diffusion flames to sub-breakdown DC and AC electric fields, as well as their superposition with ns pulse discharge waveforms in the plane-to-plane electrode geometry. The results show that sub-breakdown DC and low-frequency AC electric fields displace the flame towards the grounded electrode. However, as the AC frequency increases, the flame response becomes less pronounced. Combining a nanosecond pulse discharge with a sub-breakdown DC field generates a diffuse plasma across the entire gap. The rapid plasma self-shielding in the plane-to-plane geometry prevents the detectable flame displacement enhancement observed in other electrode geometries.
COMBUSTION AND FLAME
(2022)
Article
Biotechnology & Applied Microbiology
D. Carullo, F. Donsi, G. Ferrari, G. Pataro
Summary: The combination of high-shear homogenization and pulsed electric fields treatments efficiently recover water-soluble proteins, carbohydrates, and C-phycocyanin from A. platensis, with synergistic effects observed compared to individual treatments. High-shear homogenization promoted the breakage of microalgal trichomes, while pulsed electric fields only affected cell membrane permeabilization, and the cascade approach in combined treatments reduced the specific energy requirement for protein recovery.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Monika Sujka, Urszula Pankiewicz, Karel Flisar, Damijan Miklavcic, Sasa Haberl-Meglic
Summary: The biomass of Lactobacillus rhamnosus B 442 was subjected to continuous electroporation to improve accumulation of calcium, magnesium, and zinc. Electroporation increased accumulation of magnesium, zinc, and calcium, with a positive correlation between ion accumulation and membrane permeability for zinc and magnesium. However, an increase in calcium permeability at higher electric field strengths led to a decrease in cell viability.
BIOELECTROCHEMISTRY
(2021)
Article
Food Science & Technology
Erick Jara-Quijada, Mario Perez-Won, Gipsy Tabilo-Munizaga, Luis Gonzalez-Cavieres, Roberto Lemus-Mondaca
Summary: This review explores the use of liposomes as protection and delivery systems for drugs and functional ingredients. It discusses the effects of electric fields on lipid membranes, methods to determine the extent of electroporation, and mathematical models to analyze the mechanism. The study aims to provide insights for designing stable food liposomes under electric fields, drawing from research done in medicine, pharmacology, and the food industry.
FOOD ENGINEERING REVIEWS
(2022)
Article
Food Science & Technology
Caiyun Liu, Minming Lv, Huihui Du, Haoyu Deng, Lu Zhou, Piaoran Li, Xuxian Li, Baoguo Li
Summary: The effects of pulsed electric fields (PEF) and blanching pretreatments on frying kinetics, oil content, color, texture, acrylamide (AA) content, and microstructure were investigated. The results showed that pretreatment significantly reduced moisture ratio and oil content by 25% and 40.33% respectively. The total color change DE value of the pretreated samples was lower compared to the untreated samples. Moreover, pretreatment increased the hardness of the samples after frying, and the AA content in the fried samples pretreated with PEF + blanching was reduced by approximately 46.10% (638 mu g/kg). Finally, fried sweet potato chips obtained by the combined pretreatment exhibited a smoother and flatter cross-sectional microstructure.
Article
Food Science & Technology
N. Lopez-Giral, R. Lopez, P. Santamaria, L. Gonzalez-Arenzana, T. Garde-Cerdan
Summary: The effects of pulsed electric fields on the physico-chemical and phenolic composition of must and wine were studied. The results showed that there were no differences in color characteristics, total polyphenol index, and tannin content between the pulsed electric fields treated sample with 2 days of maceration and the control-NM sample, except for Tempranillo wines. Graciano wines from PEF samples had higher concentrations of total stilbenes, trans-resveratrol, and trans-piceid compared to control wines. The study enhances the understanding of using pulsed electric fields to produce red wines with reduced maceration time.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Review
Environmental Sciences
Akshita Singh, Nachiket Kelkar, Kannan Natarajan, Subbalaxmi Selvaraj
Summary: This report explores the potential of using organic waste in India as a calcium reservoir to address calcium deficiency issues. Food waste in India contains significant amounts of bioavailable calcium, while the population has lower calcium intake levels compared to the global average, increasing the risk of calcium deficiency-related diseases.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Applied
Min Wang, Jianjun Zhou, Juan Manuel Castagnini, Houda Berrada, Francisco J. Barba
Summary: This study investigated the effects of pulsed electric field, solvent, and time on the extraction of Chlorella antioxidant bio-molecules and minerals. The results showed that PEF treatment increased the biomolecules recovery, and using water as the extraction solvent for 120 minutes obtained more proteins and polyphenols, while using 50% DMSO obtained more chlorophyll a and b, and carotenoids.
Article
Food Science & Technology
Se-Ho Jeong, Young-Min Jung, Siyeon Kim, Jong-Hun Kim, Hyunho Yeo, Dong-Un Lee
Summary: This study investigated the effects of pulse electric field (PEF) treatment on the tenderization of beef semitendinosus muscle. An adjustable PEF chamber was designed to directly contact the beef sample without water as the transmitting medium. PEF treatment induced structural changes in beef muscle, and the degree of changes depended on the electric field strength. At 2.0 kV/cm, cutting force, hardness, and chewiness significantly decreased, and troponin-T was extensively degraded. Under a certain intensity (1.0 kV/cm), PEF treatment retained the fresh quality factors such as color and lipid oxidation.
Article
Food Science & Technology
Si-Yeon Kim, Byung-Min Lee, Seok-Young Hong, Hyun-Ho Yeo, Se-Ho Jeong, Dong-Un Lee
Summary: This study investigated the effects of pulsed electric field (PEF) treatment on the drying and rehydration kinetics, microstructure, and carotenoid content of carrot. The results showed that PEF treatment reduced drying time, improved rehydration ability, and preserved carotenoid content.
Article
Engineering, Chemical
Barbara Surano, Graciela Leiva, Guillermo Marshall, Felipe Maglietti, Carolina Schebor
Summary: By utilizing PEF technology, natural pigments and bioactive compounds can be effectively extracted from prickly pear fruit, resulting in increased juice yield and nutritional properties, suitable for industrial applications.
JOURNAL OF FOOD ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)