期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 49, 期 5, 页码 1110-1116出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2011.09.009
关键词
Pacific cod (Gadus macrocephalus) skin gelatin; Bioactive peptides; Antioxidants RAW 264.7 cells; Angiotensin-I converting enzyme inhibition
资金
- Marine Bioprocess Research Center of the Marine Bio 21 Center
- Ministry of Land, Transport and Maritime, Republic of Korea
Potent antioxidative peptides were purified from Pacific cod (Gadus macrocephalus) skin gelatin using alcalase, neutrase, papain, trypsin, pepsin, and et-chymotrypsin. Among them, the papain hydrolysate exhibited the highest antioxidant activity. Therefore, it was further purified and obtained two peptides with amino acid sequences of Thr-Cys-Ser-Pro (388 Da) and Thr-Gly-Gly-Gly-Asn-Val (485.5 Da). The antioxidant activity of the purified peptides was performed by electron spin resonance technique. Moreover, their intracellular free radical scavenging activity using 2',7'-dichlorofluorescin diacetate and the protective effect against oxidation-induced DNA damage were evaluated in mouse macrophages (RAW 264.7 cells). Furthermore, both peptides have shown potential angiotensin-I converting enzyme inhibitory effect. The present study demonstrated that the peptides derived from Pacific cod (G. macrocephalus) skin gelatin could be used in the food industry as functional ingredients with potent antioxidative and antihypertensive benefits. (C) 2011 Elsevier B.V. All rights reserved.
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