期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 49, 期 3, 页码 362-368出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2011.05.015
关键词
Astragalus polysaccharide; Peripheral blood lymphocytes; Pig; FMDV vaccine
资金
- National Twelve-Five Technological Supported Plan of China [2011BAD34B01]
- PHR (IHLB)
- Research Fund for the Doctoral Program of Higher Education of China
- public service sectors agriculture research projects [201003060-9/10]
The effects of Astragalus polysaccharides (APS) on the immune response in pigs immunized with foot- and-mouth disease virus (FMDV) vaccine were investigated. Fifteen pigs were randomly divided into five groups. Four groups were vaccinated with a FMDV inactivated vaccine. Pigs in three experimental groups were administered varying doses of APS (APS1, 5 mg/kg; APS2, 10 mg/kg; APS3, 20 mg/kg). The influence of APS on the number of CD3(+)CD4(-)CD8(+) cytotoxic T cells, CD3(+)CD4(+)CD8(+) T helper memory cells, and CD3(-)CD4(-)CD8(+) natural killer cells among peripheral blood lymphocytes (PBL) in the three APS groups were significant compared to the vaccine group. In vitro stimulation of PBL by Con A and LPS in APS groups induced a stronger proliferative response at 2 and 6 weeks post-inoculation (PI). APS markedly increased the titer of FMDV-specific antibody in a dose-dependent manner, and up-regulated mRNA expression of IFN-gamma and IL-6. APS could potentially be used as an immunomodulator for a FMDV vaccine and provide better protection against FMDV. (C) 2011 Elsevier B.V. All rights reserved.
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