Article
Biochemistry & Molecular Biology
Koichi Fujisawa, Maina Wakazaki, Aya Matsuzaki, Toshihiko Matsumoto, Naoki Yamamoto, Takafumi Noma, Taro Takami
Summary: The balance between oxidative phosphorylation and glycolysis is crucial for cancer cell growth and survival. In this study, the researchers focused on adenylate kinase 3 (AK3), an enzyme in the mitochondrial matrix that regulates adenine nucleotide metabolism. The knockout of AK3 in HeLa cells resulted in reduced proliferation and increased G1 phase cells. Metabolomic analysis showed decreased ATP levels and changes in glycolytic and TCA cycle metabolites. Additionally, AK3KO cells exhibited increased mitochondrial DNA and oxidative stress levels. The study concluded that increased expression of PCK2 may compensate for the deficiency of GDP caused by AK3 knockout.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Max-Hinderk Schuler, Alyssa M. English, Tianyao Xiao, Thane J. Campbell, Janet M. Shaw, Adam L. Hughes
Summary: Amino acids are crucial for cellular function, but elevated levels can lead to cellular toxicity. The mitochondrial-derived compartment (MDC) has been identified as a protective mechanism against amino acid stress, promoting amino acid catabolism and maintaining homeostasis through nutrient transporter remodeling.
Review
Biochemistry & Molecular Biology
Koichi Fujisawa
Summary: Adenylate kinase (AK) plays a crucial role in regulating adenine nucleotide metabolism and has been found to be associated with various diseases. This review article provides a summary of the physiological roles of AK isozymes, focusing on the symptoms caused by mutated AK isozymes in humans and phenotypic changes in animal models. The authors emphasize the importance of further research on intracellular, extracellular, and intercellular energy metabolism with a focus on AK for advancing new therapeutic approaches for diseases like cancer, lifestyle-related diseases, and aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Song Zhang, Satsuki Yamada, Sungjo Park, Aleksandr Klepinin, Tuuli Kaambre, Andre Terzic, Petras Dzeja
Summary: The study revealed that AK2 plays a crucial role in early embryonic development and its loss in adults can lead to sudden heart failure, which is eventually recovered with an adaptive response. The compensatory upregulation of related enzymes and genes highlights the innate plasticity of the AK2-integrated bioenergetics system in maintaining homeostasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Nils Grotehans, Lynn McGarry, Hendrik Nolte, Vanessa Xavier, Moritz Kroker, alvaro Jesus Narbona-Perez, Soni Deshwal, Patrick Giavalisco, Thomas Langer, Thomas MacVicar
Summary: Replication and expression of mitochondrial genes rely on a sufficient supply of nucleotides. Dysregulated nucleotide metabolism affects mitochondrial genome stability and transcription. This study reveals the role of a mitochondrial nucleoside diphosphate kinase, NME6, in providing pyrimidine ribonucleotides for mitochondrial gene transcription. Loss of NME6 function leads to depletion of mitochondrial transcripts and impaired oxidative phosphorylation, which can be rescued by exogenous pyrimidine ribonucleoside supply. NME6 is also crucial for mitochondrial DNA maintenance when cytosolic pyrimidine deoxyribonucleotide access is limited.
Article
Oncology
Bin Li, Junyi Wang, Jing Liao, Minghui Wu, Xiangshu Yuan, Hezhi Fang, Lijun Shen, Minghua Jiang
Summary: This study investigates the role of YY1 gene in the development of pancreatic ductal adenocarcinoma (PDAC). The elevated expression of YY1 is found in human PDAC tissues, and it is positively associated with poor disease progression. YY1 deletion inhibits PDAC cell proliferation and tumorigenicity by impairing mitochondrial function and altering cellular nucleotide homeostasis. Targeting nucleotide biosynthesis may be a promising strategy for PDAC treatment.
CANCER CELL INTERNATIONAL
(2022)
Article
Cell Biology
Chuan-Yang Dai, Chai Chee Ng, Grace Ching Ching Hung, Ina Kirmes, Laetitia A. A. Hughes, Yunguang Du, Christopher A. A. Brosnan, Arnaud Ahier, Anne Hahn, Cole M. M. Haynes, Oliver Rackham, Aleksandra Filipovska, Steven Zuryn
Summary: The ability to balance conflicting functional demands is critical for organismal survival. In Caenorhabditis elegans, the bZIP transcription factor ATFS-1 promotes mtDNA repair by interfering with mitochondrial pre-initiation transcription complex assembly, leading to enhanced functional longevity of cells and protection against decline in animal behavior caused by mtDNA damage.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Meixiao Zhan, Yufeng Ding, Shanzhou Huang, Yuhang Liu, Jing Xiao, Hua Yu, Ligong Lu, Xiongjun Wang
Summary: By phosphorylating and stabilizing DHODH, sensitivity of liver cancer cells to Oxaliplatin is enhanced.
NATURE COMMUNICATIONS
(2023)
Article
Physiology
Sevasti Zervou, Debra J. McAndrew, Hannah J. Whittington, Hannah A. Lake, Kyung Chan Park, Kuan Minn Cha, Philip J. Ostrowski, Thomas R. Eykyn, Jurgen E. Schneider, Stefan Neubauer, Craig A. Lygate
Summary: The study suggests that modest elevation of AK1 activity may improve functional recovery following ischemia/reperfusion injury, but has unexpected impacts on left ventricular weight, function, and metabolite levels under basal resting conditions.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jacob M. Winter, Tarun Yadav, Jared Rutter
Summary: Mitochondrial energetics and respiration play crucial roles in the apoptotic response of cancer cells. Exploring the functional connection between these processes can provide insights into enhancing apoptotic stress in cancer therapy. Targeting respiration or downstream signaling pathways and understanding the co-localization of respiration and apoptotic regulation in the same organelle are important research directions.
Article
Multidisciplinary Sciences
Caroline E. Dewar, Silke Oeljeklaus, Jan Mani, Wignand W. D. Muehlhaeuser, Corinne von Kaenel, Johannes Zimmermann, Torsten Ochsenreiter, Bettina Warscheid, Andre Schneider
Summary: Mitochondrial quality control mechanism in Trypanosoma brucei is characterized by the ablation of ATOM69, which triggers a unique pathway resulting in the degradation of non-imported mitochondrial proteins. This process involves the recruitment of an unknown protein, an E3 ubiquitin ligase, and a ubiquitin-like protein (TbUbL1) and the release of TbUbL1 from the nucleus to the cytosol.
NATURE COMMUNICATIONS
(2022)
Article
Infectious Diseases
Guoqing Shao, Ruiqi Hua, Hongyu Song, Yanxin Chen, Xiaowei Zhu, Wei Hou, Shengqiong Li, Aiguo Yang, Guangyou Yang
Summary: The study demonstrates that the six recombinant proteins have significant protective efficacy in dogs against E. granulosus infection, reducing worm biomass and inhibiting growth. The vaccinated dogs also showed an increased level of IgG, indicating an enhanced immune response.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Naintara Jain, Ridhima Gomkale, Olaf Bernhard, Peter Rehling, Luis Daniel Cruz-Zaragoza
Summary: Mitochondria play a crucial role in cellular energy production and metabolism, and errors in mitochondrial protein import can lead to metabolic disorders. This study establishes a fluorescence-based import assay to analyze protein import into mitochondria, offering the advantage of quantifying import with high sensitivity. The assay can be adapted to a screening-compatible format and used to monitor the assembly of the F1F0 ATP synthase in purified mitochondria.
Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
F. X. Reymond Sutandy, Ines Goessner, Georg Tascher, Christian Muench
Summary: This study demonstrates that cytosol-mitochondrial reactive oxygen species (mtROS) and accumulation of mitochondrial protein precursors in the cytosol (c-mtProt) play a critical role in initiating the mitochondrial unfolded protein response (UPRmt). The findings reveal a link between mitochondrial and cytosolic proteostasis and provide molecular insight into UPRmt signaling in human cells.
Article
Oncology
Haiyang Jiang, Lei Zhao, Xiaoshen Dong, Anning He, Caiwei Zheng, Magnus Johansson, Anna Karlsson, Xinyu Zheng
Article
Multidisciplinary Sciences
Stefano Bartesaghi, Vincenzo Graziano, Sara Galavotti, Nick V. Henriquez, Joanne Betts, Jayeta Saxena, A. Deli, Anna Karlsson, L. Miguel Martins, Melania Capasso, Pierluigi Nicotera, Sebastian Brandner, Vincenzo De Laurenzi, Paolo Salomoni
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Oncology
Xiaoshan Zhou, Joao A. Paredes, Shuba Krishnan, Sophie Curbo, Anna Karlsson
Article
Oncology
Xiaoshan Zhou, Sophie Curbo, Fuqiang Li, Shuba Krishnan, Anna Karlsson
Article
Biochemistry & Molecular Biology
Sophie Curbo, Kathrin Reiser, Anna-Klara Rundlof, Anna Karlsson, Mathias Lundberg
ANTIOXIDANTS & REDOX SIGNALING
(2013)
Article
Oncology
Xiaoshen Dong, Wenzhi Qu, Shuai Ma, Zhi Zhu, Caiwei Zheng, Anning He, Anna Karlsson, Ke Xu, Xinyu Zheng
Article
Biochemistry & Molecular Biology
Kathrin Reiser, Katrien O. Francois, Dominique Schols, Tomas Bergman, Hans Jornvall, Jan Balzarini, Anna Karlsson, Mathias Lundberg
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Marjan Amiri, Francesca Conserva, Christakis Panayiotou, Anna Karlsson, Nicola Solaroli
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2013)
Review
Biochemistry & Molecular Biology
Christakis Panayiotou, Nicola Solaroli, Anna Karlsson
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Jan Balzarini, Federico Gago, Wim Kulik, Andre B. P. van Kuilenburg, Anna Karlsson, Matt A. Peterson, Morris J. Robins
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Shuba Krishnan, Xiaoshan Zhou, Joao A. Paredes, Raoul V. Kuiper, Sophie Curbo, Anna Karlsson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Shuba Krishnan, Joao A. Paredes, Xiaoshan Zhou, Raoul V. Kuiper, Kjell Hultenby, Sophie Curbo, Anna Karlsson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Editorial Material
Clinical Neurology
Charlotte L. Alston, Andrew M. Schaefer, Pravrutha Raman, Nicola Solaroli, Kim J. Krishnan, Emma L. Blakely, Langping He, Kate Craig, Mark Roberts, Aashish Vyas, John Nixon, Rita Horvath, Douglass M. Turnbull, Anna Karlsson, Grainne S. Gorman, Robert W. Taylor
Article
Multidisciplinary Sciences
Xiaoshan Zhou, Kristina Kannisto, Sophie Curbo, Ulrika von Dobeln, Kjell Hultenby, Sindra Isetun, Mats Gafvels, Anna Karlsson
Article
Multidisciplinary Sciences
Kathrin Reiser, Leen Mathys, Sophie Curbo, Christophe Pannecouque, Sam Noppen, Sandra Liekens, Lars Engman, Mathias Lundberg, Jan Balzarini, Anna Karlsson
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)