Review
Biochemistry & Molecular Biology
Mounia Tahri-Joutey, Pierre Andreoletti, Sailesh Surapureddi, Boubker Nasser, Mustapha Cherkaoui-Malki, Norbert Latruffe
Summary: This article discusses the shared ability of mitochondria and peroxisomes in degrading fatty acid chains, the hepatomegaly effect caused by clofibrate, the identification of peroxisome proliferators and their impact on the peroxisomal fatty acid beta-oxidation pathway, and the modulation of PPAR alpha function by dietary micronutrients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Maral Hajipour, Khatereh Mokhtari, Mohammad Mahdevar, Maryam Esmaeili, Maryam Peymani, Mohammad Hossein Nasr-Esfahani, Sepideh Mirzaei, Mehrdad Hasehmi, Kiavash Hushmandi, Kamran Ghaedi
Summary: This study identified lncRNAs affected by PPARγ activation through bioinformatics analysis and found that treatment with PIO significantly increased the expression levels of related genes and lncRNAs, providing a new strategy for understanding the function and activity of PPARγ in colon cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Endocrinology & Metabolism
Yijun Lin, Lingling Chen, Xue You, Zixuan Li, Chenchen Li, Yan Chen
Summary: The study demonstrated the regulatory role of PAQR9 in hepatic ketogenesis and showed that deletion of PAQR9 attenuated ketogenesis and fatty acid oxidation during fasting by decreasing PPARa protein stability and competing with HUWE1 for interaction with PPAR alpha.
MOLECULAR METABOLISM
(2021)
Article
Medicine, Research & Experimental
Pouneh K. Fazeli, Miriam A. Bredella, Gisela Pachon-Pena, Wenxiu Zhao, Xun Zhang, Alexander T. Faje, Megi Resulaj, Sai P. Polineni, Tara M. Holmes, Hang Lee, Elizabeth K. O'Donnell, Ormond A. MacDougald, Mark C. Horowitz, Clifford J. Rosen, Anne Klibanski
Summary: The study found that vertebral BMAT significantly increased during high-calorie feeding and fasting, while ghrelin levels decreased and were inversely associated with changes in BMAT. High-calorie feeding induces an immune response in bone marrow similar to peripheral adipose tissue.
Article
Chemistry, Multidisciplinary
Matthew C. Fleming, Matthew M. Bowler, Rodney Park, Konstantin I. Popov, Albert A. Bowers
Summary: mRNA display is a powerful technique for discovering high-affinity ligands, but limited cyclization chemistries are compatible with it. Here, we demonstrate that peptides containing tyrosine and cysteine can be rapidly cyclized using the copper-dependent oxidase tyrosinase. This tyrosinase-mediated cyclization combined with mRNA display enables the discovery of potent macrocyclic ligands for MAGE-A4 with nanomolar IC50 values, showing a significant advantage over noncyclized analogues.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Xiao Zhang, Ting Gao, Senwen Deng, Lin Shang, Xiaocui Chen, Kai Chen, Ping Li, Xiaojuan Cui, Jia Zeng
Summary: Fasting induces lipid accumulation in the liver, with endogenous dicarboxylic acids playing a role in controlling mitochondrial beta-oxidation. Excessive beta-oxidation of dicarboxylic acids leads to succinate production, which suppresses mitochondrial beta-oxidation, promotes hepatic lipid deposition, and identifies cross talk between peroxisomal and mitochondrial fatty acid oxidation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Golnaz Kamalinia, Brian J. Grindel, Terry T. Takahashi, Steven W. Millward, Richard W. Roberts
Summary: mRNA display is a powerful platform for directed evolution of proteins and peptides, linking phenotype and genotype through puromycin. Iterative selection cycles lead to peptides with desired functional levels. This review discusses the development and broad application of mRNA display technology in selecting novel peptides and proteins.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Biology
Abdulaziz S. Saeedan, Gamal A. Soliman, Rehab F. Abdel-Rahman, Reham M. Abd-Elsalam, Hanan A. Ogaly, Khalid M. Alharthy, Maged S. Abdel-Kader
Summary: This study found that the combination of glyburide and Artemisia judaica extract significantly improved glycemic control in male Wistar rats, outperforming glyburide monotherapy.
Article
Multidisciplinary Sciences
Ryo Kawakami, Hiroaki Sunaga, Tatsuya Iso, Ryosuke Kaneko, Norimichi Koitabashi, Masaru Obokata, Tomonari Harada, Hiroki Matsui, Tomoyuki Yokoyama, Masahiko Kurabayashi
Summary: The study found that fasting-induced β-OHB and FGF21 coordinately regulate oxidative stress response gene expression in the heart by modulating the expression of PPARα and oxidative stress response genes. Additionally, the absence of FGF21 exacerbates the expression levels of β-OHB and PPG1α in the heart.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Sabrina E. Iskandar, Lilly F. Chiou, Tina M. Leisner, Devan J. Shell, Jacqueline L. Norris-Drouin, Cyrus Vaziri, Kenneth H. Pearce, Albert A. Bowers
Summary: This study reports a method to identify covalent cyclic peptide inhibitors in mRNA display. By combining co- and post-translational library diversification strategies, cyclic libraries with reactive dehydroalanines (Dhas) were created and used for selections against two model targets. The most potent hits showed low nanomolar inhibitory activities and disrupted known protein-protein interactions with their selected targets. Overall, this study establishes Dhas as electrophiles for covalent inhibition and demonstrates the synergy of separate library diversification methods in mRNA display for novel applications like covalent inhibitor discovery.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Medicine, Research & Experimental
Huan Wang, Tracey W. Chan, Ajay A. Vashisht, Brian G. Drew, Anna C. Calkin, Thurl E. Harris, James A. Wohlschlegel, Xinshu Xiao, Karen Reue
Summary: Lipin 1 regulates cellular lipid homeostasis through roles in glycerolipid synthesis and transcriptional coactivation, but its deficiency can lead to episodic disease symptoms triggered by metabolic stress. Fasting exacerbates the deleterious effects of lipin 1 deficiency on metabolic homeostasis, mainly through its influence on mRNA splicing in the liver.
Article
Neurosciences
Carl G. Gottschalk, Malabendu Jana, Avik Roy, Dhruv R. Patel, Kalipada Pahan
Summary: Research suggests that gemfibrozil can protect dopaminergic neurons in an animal model of PD, increase neurotransmitters in the striatum, and improve locomotor activities.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Donna D. Yu, Gregg Van Citters, Hongzhi Li, Brian M. Stoltz, Barry M. Forman
Summary: The study demonstrates that the novel selective PPAR alpha modulators DY series activate PPAR alpha by up-regulating PPAR alpha target genes directly involved in NAFLD and NASH. The design and evaluation of these modulators provide a new approach for the treatment of NAFLD and NASH.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Xinlei Liu, Yan Zhang, Chunya Ma, Juntang Lin, Jiang Du
Summary: Alternate day fasting (ADF) attenuated high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) in mice by reducing hepatic inflammation and lipid accumulation. Fasting increased expression of serum and hepatic fibroblast growth Factor 21 (Fgf21) and prevented FFA-induced lipid storage in hepatocytes. Refeeding inhibited the increase in Fgf21 expression induced by fasting, suggesting a potential role of PPAR alpha/Fgf21 signaling in protecting against NAFLD.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Tomomi Yamazaki, Megumi Ihato
Summary: PPAR-alpha prevents fatty liver caused by excessive sucrose intake through regulating SREBP-1c target gene expression and liver TG accumulation. Gender differences may be related to the expression level of PPAR-gamma.
Review
Cell & Tissue Engineering
Deepak Narayanan Iyer, Wai-Yan Sin, Lui Ng
WORLD JOURNAL OF STEM CELLS
(2019)
Article
Oncology
Deepak Narayanan Iyer, Timothy Ming-Hun Wan, Johnny Hon-Wai Man, Ryan Wai-Yan Sin, Xue Li, Oswens Siu-Hung Lo, Dominic Chi-Chung Foo, Roberta Wen-Chi Pang, Wai-Lun Law, Lui Ng
Article
Biochemical Research Methods
Kai Wang, Martin Yan-Chun Chan, Jian Xu, Pik-Shan Li, Xing Liu, Angus Yiu-Fai Lee, Susanna Sau-Tuen Lee, Wing-Tai Cheung
TRANSGENIC RESEARCH
(2020)
Review
Endocrinology & Metabolism
Kristy T. K. Lau, Lui Ng, Jason W. H. Wong, Herbert H. F. Loong, Wendy W. L. Chan, Chi Ho Lee, Carlos K. H. Wong
Summary: Sodium-glucose co-transporter 2 inhibitors have shown potential anticancer activities in selected cancer types, possibly through mechanisms like reducing glucose uptake into cancer cells and modulating signaling pathways. Clinical findings suggest that canagliflozin and dapagliflozin may have anticancer properties in patients with liver and colon cancers.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2021)
Article
Cell & Tissue Engineering
Ryan Wai-Yan Sin, Dominic Chi-Chung Foo, Deepak Narayanan Iyer, May Sau-Yee Fan, Xue Li, Oswens Siu-Hung Lo, Wai-Lun Law, Lui Ng
Summary: <br>Gene expression changes are inevitable in cancer development, and this study aimed to investigate the potential of rectal swab specimens as a tool for detecting dysregulation of cancer stem cells and evaluating it as a screening method. The study found that a panel of Oct4 and CD26 could distinguish CRC from normal and polyp patients, while a panel of c-MYC and CXCR4 may identify polyp and CRC patients from normal individuals.
STEM CELLS INTERNATIONAL
(2021)
Article
Oncology
Lui Ng, Dominic Chi-Chung Foo, Carlos King-Ho Wong, Abraham Tak-Ka Man, Oswens Siu-Hung Lo, Wai-Lun Law
Summary: This study investigated the potential of DPP4-inhibitors in treating CRC patients, and found that DPP4-inhibitor treatment was associated with a significantly better 5-year prognosis following curative resection compared to metformin treatment. The study also showed an association between patient prognosis and immune cell population features, suggesting that immune cell profiles may serve as a biomarker for predicting patient prognosis in response to DPP4-inhibitor treatment.
Article
Oncology
Lui Ng, Xue Li, Timothy Ming-Hun Wan, Deepak Iyer, Ryan Wai-Yan Sin, Oswens Siu-Hung Lo, Dominic Chi-Chung Foo, Wai-Lun Law
Summary: This study identified dysregulated miRNAs during the stepwise malignant transformation of colorectal mucosal cells and their association with immune cell populations.
Article
Biochemistry & Molecular Biology
Lui Ng, Sunny Kit-Man Wong, Zheng Huang, Colin Siu-Chi Lam, Ariel Ka-Man Chow, Dominic Chi-Chung Foo, Oswens Siu-Hung Lo, Roberta Wen-Chi Pang, Wai-Lun Law
Summary: This study revealed the functional roles of CD26 in inducing colorectal cancer migration, invasion, angiogenesis and metastasis, and identified the potential involvement of MMP1 and CAV1 in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Deepak Narayanan Iyer, Dominic Chi-Chung Foo, Oswens Siu-Hung Lo, Timothy Ming-Hun Wan, Xue Li, Ryan Wai-Yan Sin, Roberta Wen-Chi Pang, Wai-Lun Law, Lui Ng
Summary: MiR-509-3p is overexpressed in colorectal cancer and associated with aggressive disease presentation. It inhibits the expression of tumor suppressor PHLPP2 by directly binding to its 3'UTR. The high expression of miR-509-3p in tumor-adjacent normal tissues is associated with poor prognosis in CRC.
Article
Cell Biology
Lui Ng, Timothy Ming-Hun Wan, Deepak Narayanan Iyer, Zheng Huang, Ryan Wai-Yan Sin, Abraham Tak-Ka Man, Xue Li, Dominic Chi-Chung Foo, Oswens Siu-Hung Lo, Wai-Lun Law
Summary: This study revealed that despite its role as a tumor suppressor in colorectal cancer (CRC), high levels of miR-187-3p in tumors were associated with poor prognosis and higher levels of peripheral inflammation-related blood markers.
Article
Cell Biology
Lui Ng, Hung-Sing Li, Abraham Tak-Ka Man, Ariel Ka-Man Chow, Dominic Chi-Chung Foo, Oswens Siu-Hung Lo, Roberta Wen-Chi Pang, Wai-Lun Law
Summary: High expression of CBX8 gene in adjacent normal tissue is associated with poor prognosis for tumor progression and survival in colorectal cancer patients.
Article
Biochemistry & Molecular Biology
Lui Ng, Ryan Wai-Yan Sin, David Him Cheung, Wai-Keung Leung, Abraham Tak-Ka Man, Oswens Siu-Hung Lo, Wai-Lun Law, Dominic Chi-Chung Foo
Summary: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease that is increasing worldwide, and it is associated with colorectal polyps. This study aimed to investigate the potential of serum microRNAs (miRNAs) in identifying NAFLD in colorectal polyp patients. The results showed that a serum miRNA panel composed of four miRNA pairs significantly identified NAFLD in colorectal polyp patients, and its performance improved when patients with other concurrent metabolic disorders were removed from the analysis. The serum miRNA panel could be a potential diagnostic biomarker for screening NAFLD in colorectal polyp patients for early diagnosis and prevention of disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Oncology
Lui Ng, Alfons Navarro, Wai-Lun Law
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kai Wang, Yan-Chun Chan, Pui-Kin So, Xing Liu, Lu Feng, Wing-Tai Cheung, Susanna Sau-Tuen Lee, Shannon Wing-Ngor Au
Summary: This study elucidated the sulfonation of bile acids by SULT2A8 and its structural basis, revealing the functional diversity of SULTs in humans and mice.
JOURNAL OF LIPID RESEARCH
(2021)
Review
Cell Biology
Timothy Ming-Hun Wan, Deepak Narayanan Iyer, Lui Ng
HISTOLOGY AND HISTOPATHOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
