Article
Genetics & Heredity
Biao Hu, Tianju Liu, Zhe Wu, Sem H. Phan, Karen Blyth
Summary: P53 can suppress the transcription and translation of C/EBP beta gene, and regulate the translation of LAP and LIP isoforms of C/EBP beta through the expression of eIF4E and eIF4E-BP1; furthermore, the 5'UTR region plays an important role in the differential control of LAP and LIP translation of C/EBP beta.
Article
Biochemistry & Molecular Biology
JanWillem Duitman, Leonie Hartl, Joris J. T. H. Roelofs, Maarten F. Bijlsma, C. Arnold Spek
Summary: C/EBP delta is downregulated in pancreatic ductal adenocarcinoma (PDAC) cells and associated with decreased patient survival and pro-tumorigenic properties. Systemic C/EBP delta facilitates pancreatic cancer metastasis, potentially through C/EBP delta-PAFR-dependent tumor cell extravasation.
Article
Biochemistry & Molecular Biology
Atsuko Kitano, Toshio Norikura, Isao Matsui-Yuasa, Hiromi Shimakawa, Makoto Kamezawa, Akiko Kojima-Yuasa
Summary: This study investigated the epigenetic regulation of black carrot extract (BCE) in protecting against ethanol-induced hepatic damage. The results showed that BCE-BuOH increased intracellular cAMP levels by suppressing the expression of PDE4b, and also inhibited the acetylation of H3K9 through an increase in Sirt1. Additionally, BCE-BuOH treatment increased the level of MAT2a mRNA and intracellular S-adenosylmethionine. Overall, these findings demonstrate the potential usefulness of BCE-BuOH in protecting against alcohol-induced hepatic injury.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
HaiXu Song, Jiahao Liu, Xiaoxiang Tian, Dan Liu, Jiayin Li, Xiaojie Zhao, Zhu Mei, Chenghui Yan, Yaling Han
Summary: The transcription factor CCAAT/enhancer-binding protein ? (C/EBP?) plays a crucial role in the differentiation and cell cycle control of megakaryocytes (MKs), potentially aiding in the treatment of thrombocytopenia and other platelet-related disorders.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Multidisciplinary Sciences
Romila Mascarenhas, Markus Ruetz, Harsha Gouda, Natalie Heitman, Madeline Yaw, Ruma Banerjee
Summary: G-proteins and their cofactors MMAA and MMAB orchestrate cofactor translocation and repair in B-12-dependent human MMUT, and their crystal structure reveals a dramatic conformational change and the basis of GTPase activation. Disease-causing mutations at the MMAA-MMUT interfaces are explained by the structure.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Harsha Gouda, Zhu Li, Markus Ruetz, Ruma Banerjee
Summary: This study determined the redox potential of cob(II)alamin and revealed its thermodynamic gain by enforcing a specific geometric configuration. It also identified mitochondrial adrenodoxin as an electron donor to the enzyme MMAB. The study demonstrated the importance of coordination control in synthesizing 5'-deoxyadenosylcobalamin and uncovered the pathogenic mechanism in MMAB patient variants. These findings provide insights into the redox chemistry and functional mechanisms of cobalamin-dependent enzymes.
INORGANIC CHEMISTRY
(2023)
Article
Cell & Tissue Engineering
Neena Lala-Tabbert, Hamood AlSudais, Francois Marchildon, Dechen Fu, Nadine Wiper-Bergeron
Summary: The study shows that the transcription factor C/EBP beta is necessary to maintain quiescence of satellite cells in uninjured muscle. Loss of C/EBP beta in satellite cells promotes premature exit from quiescence, while forced expression of C/EBP beta inhibits proliferation by upregulating quiescence-associated genes. Additionally, caveolin-1 is identified as a direct transcriptional target of C/EBP beta required for cell cycle exit in muscle satellite cells expressing C/EBP beta.
Article
Biochemistry & Molecular Biology
Andre M. Ribeiro-dos-Santos, Megan S. Hogan, Raven D. Luther, Ran Brosh, Matthew T. Maurano
Summary: This study investigates the impact of genomic context on enhancer blocker activity, using integrated reporter constructs and analyzing reporter activity based on genomic position. The study reveals that genomic context influences the expression patterns of enhancer blocker reporter integrations, with some regions showing robust expression. Moreover, the study shows that CTCF insulator elements do not completely abolish the effects of integrated reporters on endogenous gene expression.
Article
Gastroenterology & Hepatology
Hui Peng, Huiting Xu, Jie Wu, Jiangyuan Li, Yi Zhou, Zehuan Ding, Stefan K. Siwko, Xianglin Yuan, Kevin L. Schalinske, Gianfranco Alpini, Ke K. Zhang, Linglin Xie
Summary: This study revealed that maternal high-fat diet disrupts the methionine cycle and one-carbon metabolism in offspring livers, leading to changes in lipid homeostasis. Specific genes involved in one-carbon metabolism modified by different maternal diets were identified.
LIVER INTERNATIONAL
(2021)
Article
Physiology
Pengyuan Chen, Wanzi Hong, Ziying Chen, Flora Gordillo-Martinez, Siying Wang, Hualin Fan, Yuanhui Liu, Yining Dai, Bo Wang, Lei Jiang, Hongjiao Yu, PengCheng He
Summary: The transcription factor C/EBP alpha plays a crucial role in the osteochondrogenic transdifferentiation of vascular smooth muscle cells (VSMCs) and vascular calcification. C/EBP alpha regulates the expression of the osteochondrogenic gene SOX9, and its overexpression or knockdown affects VSMC calcification. Silencing SOX9 expression significantly inhibits phosphate- and calcium-induced VSMC calcification.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaolong Wei, Megan. A. A. Murphy, Nihal. A. A. Reddy, Yi Hao, Taylor. G. G. Eggertsen, Jeffrey. J. J. Saucerman, Irina. M. M. Bochkis
Summary: This study suggests that NAFLD, which is associated with aging and age-dependent metabolic changes, is related to regulation of heterochromatin at the nuclear lamina. Changes at the nuclear lamina lead to redistribution of LADs, altered binding of pioneer factor FOXA2, and up-regulation of lipid synthesis and storage, resulting in the development of NAFLD.
Article
Biochemistry & Molecular Biology
Qiushuang Li, Qiongxian Yan, Chuanshe Zhou, Shaoxun Tang, Xuefeng Han, Zhiliang Tan
Summary: The study found that feeding pregnant goats with methionine-chelated zinc increased the offspring liver organ index without affecting genomic DNA methylation. Blood zinc content decreased in offspring, and genes related to methylation were downregulated in the liver and spleen, leading to enhanced development and function of the immune system in offspring.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Endocrinology & Metabolism
Daisuke Aibara, Kohei Matsuo, Kimihiko Matsusue
Summary: This study investigates the mechanism by which C/EBP alpha regulates the Lipn gene in the mouse liver. The results suggest that C/EBP alpha and type 2 diabetic environment may be required for hepatic Lipn expression.
Article
Endocrinology & Metabolism
Daisuke Aibara, Kohei Matsuo, Kimihiko Matsusue
Summary: CCAAT/enhancer-binding protein alpha (C/EBP alpha) is a transcription factor abundantly expressed in the liver and white adipose tissue (WAT). This study investigates the mechanism by which C/EBP alpha regulates the lipase family member N (Lipn) gene in the mouse liver. The results suggest that C/EBP alpha and type 2 diabetic environment may be required for hepatic Lipn expression. Two functional CEBPREs in the Lipn promoter region are essential for enhancing Lipn transcriptional activity by C/EBP alpha.
Article
Biochemistry & Molecular Biology
Bing Huang, Weihao Zhao, Xue Cai, Yumin Zhu, Yingxian Lu, Junli Zhao, Nan Xiang, Xiaofei Wang, Hu Deng, Xiaping Tang, Lingyu Liu, Yanyu Zhao, Yigong Shi
Summary: The harmful effects of leaked electromagnetic radiation (EMR) on human health, particularly on oligodendrocytes, have raised concerns due to the rapid growth of wireless electronic devices. Through experiments, it was found that pulse-modulated EMR significantly increased the mRNA level of C/EBP beta in oligodendrocytes and altered the expression and functions of its related genes and proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Yuchang Li, Ingrid Lua, Samuel W. French, Kinji Asahina
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2016)
Review
Gastroenterology & Hepatology
Ingrid Lua, Kinji Asahina
Article
Gastroenterology & Hepatology
Ingrid Lua, Yuchang Li, Jessica A. Zagory, Kasper S. Wang, Samuel W. French, Jean Sevigny, Kinji Asahina
JOURNAL OF HEPATOLOGY
(2016)
Article
Surgery
Marie V. Nguyen, Jessica A. Zagory, William H. Dietz, Alex Park, Michael Fenlon, Menghan Zhao, Jiabo Xu, Ingrid Lua, Nirmala Mavila, Kinji Asahina, Kasper S. Wang
Article
Anatomy & Morphology
Tomohiro Ogawa, Yuchang Li, Ingrid Lua, Andrea Hartner, Kinji Asahina
DEVELOPMENTAL DYNAMICS
(2018)
Meeting Abstract
Surgery
Michael Fenlon, Jiabo Xu, Kinji Asahina, Kasper S. Wang
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2018)
Article
Gastroenterology & Hepatology
Steven Balog, Yuchang Li, Tomohiro Ogawa, Toshio Miki, Takeshi Saito, Samuel W. French, Kinji Asahina
Article
Gastroenterology & Hepatology
Kinji Asahina, Steven Balog, Edward Hwang, Eugene Moon, Emily Wan, Kaitlin Skrypek, Yibu Chen, Jay Fernandez, Janet Romo, Qihong Yang, Keane Lai, Samuel W. French, Hidekazu Tsukamoto
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kinji Asahina
Summary: This study found that zinc is essential for the growth and survival of pancreatic cancer cells, and a TSQ-zinc complex may induce cell death. The effects of TPEN and TSQ on pancreatic cancer cells differ.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Gastroenterology & Hepatology
Steven Balog, Reika Fujiwara, Stephanie Q. Pan, Khairat B. El-Baradie, Hye Yeon Choi, Sonal Sinha, Qihong Yang, Kinji Asahina, Yibu Chen, Meng Li, Matthew Salomon, Stanley W-K Ng, Hidekazu Tsukamoto
Summary: This study identified subpopulations of collagen type 1 alpha 1 (Col1a1)-expressing cells in alcoholic hepatitis (AH) and found that a highly profibrotic, inflammatory, and immunoregulatory Lrat+FbIn2+ subpopulation may contribute to the development of AH.
Article
Gastroenterology & Hepatology
Soo-Mi Kweon, Jose Irimia-Dominguez, Gayeoun Kim, Patrick T. Fueger, Kinji Asahina, Keith K. K. Lai, Daniela S. Allende, Quincy R. R. Lai, Chih-Hong Lou, Walter M. Tsark, Ju Dong Yang, Dominic S. Ng, Ju-Seog Lee, Patrick Tso, Wendong Huang, Keane K. Y. Lai
Summary: Despite being studied for over 20 years, the biological roles of midnolin in vivo are largely unknown due to the lack of a functional animal model. However, recent research has shown that knocking down midnolin suppresses liver cancer cell tumorigenicity and affects lipid metabolism. This led to the hypothesis that knocking out midnolin in vivo could potentially protect against nonalcoholic fatty liver disease (NAFLD). The study developed the first global midnolin knockout mouse model and found that heterozygous knockout mice had a reduced severity of NAFLD, suggesting midnolin as a novel therapeutic target.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Allen Zhong, Celia Short, Jiabo Xu, G. Esteban Fernandez, Nicolas Malkoff, Nicolas Noriega, Theresa Yeo, Larry Wang, Nirmala Mavila, Kinji Asahina, Kasper S. Wang
Summary: This study found that Prom1-expressing cells play a role in the restitution of biliary epithelium following injury. Null mutation of Prom1 leads to dilation of peribiliary glands and impaired cell polarity and motility.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Ingrid Lua, Steven Balog, Ami Yanagi, Chise Tateno, Kinji Asahina
Summary: This study revealed that deletion or inhibition of LPAR1 gene in hepatocytes can reduce steatosis in methionine-choline-deficient diet-induced steatohepatitis, possibly through down-regulation of fatty acid transporter CD36.
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2021)
Article
Physiology
Michael Fenlon, Celia Short, Jiabo Xu, Nicolas Malkoff, Elaa Mahdi, Michelle Hough, Alison Glazier, Calvin Lee, Kinji Asahina, Kasper S. Wang
PHYSIOLOGICAL REPORTS
(2020)
Article
Cell Biology
Lina He, James Gubbins, Zhechu Peng, Vivian Medina, Fan Fei, Kinji Asahina, Jiaohong Wang, Michael Kahn, Carl B. Rountree, Bangyan L. Stiles
FIBROGENESIS & TISSUE REPAIR
(2016)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)